The DOI matches its Crossref record; 10 of the 10 references it cites were found in a registry or an index.
Reactive astrocytes undergo M1 microglia/macrohpages-induced necroptosis in spinal cord injury
Open accessAbstract
BACKGROUND: A unique feature of the pathological change after spinal cord injury (SCI) is the progressive enlargement of lesion area, which usually results in cavity formation and is accompanied by reactive astrogliosis and chronic inflammation. Reactive astrocytes line the spinal cavity, walling off the lesion core from the normal spinal tissue, and are thought to play multiple important roles in SCI. The contribution of cell death, particularly the apoptosis of neurons and oligodendrocytes during the process of cavitation has been extensively studied. However, how reactive astrocytes are eliminated following SCI remains largely unclear. RESULTS: By immunohistochemistry, in vivo propidium iodide (PI)-labeling and electron microscopic examination, here we reported that in mice, reactive astrocytes died by receptor-interacting protein 3 and mixed lineage kinase domain-like protein (RIP3/MLKL) mediated necroptosis, rather than apoptosis or autophagy. Inhibiting receptor-interacting protein 1 (RIP1) or depleting RIP3 not only significantly attenuated astrocyte death but also rescued the neurotrophic function of astrocytes. The astrocytic expression of necroptotic markers followed the polarization of M1 microglia/macrophages after SCI. Depleting M1 microglia/macrophages or transplantation of M1 macrophages could significantly reduce or increase the necroptosis of astrocytes. Further, the inflammatory responsive genes Toll-like receptor 4 (TLR4) and myeloid differentiation primary response gene 88 (MyD88) are induced in necroptotic astrocytes. In vitro antagonizing MyD88 in astrocytes could significantly alleviate the M1 microglia/macrophages-induced cell death. Finally, our data showed that in human, necroptotic markers and TLR4/MyD88 were co-expressed in astrocytes of injured, but not normal spinal cord. CONCLUSION: Taken together, these results reveal that after SCI, reactive astrocytes undergo M1 microglia/macrophages-induced necroptosis, partially through TLR/MyD88 signaling, and suggest that inhibiting astrocytic necroptosis may be beneficial for preventing secondary SCI.
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Cites
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RIP3, an Energy Metabolism Regulator That Switches TNF-Induced Cell Death from Apoptosis to Necrosis
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Chapter 4 Cell death in models of spinal cord injury
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“Background One unique pathological change after primarily spinal cord injury (SCI) is the secondary injury, which is characterized by continuous tissue loss, reactive astrogliosis and chronic inflammation, and usually leads to gradual expansion of the lesion center and formation of a spinal cavity [1, 2].”
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Cited by
Spinal Cord Injury: Pathophysiology, Multimolecular Interactions, and Underlying Recovery Mechanisms
Anjum, A., Yazid, M. D., Fauzi Daud, M., Idris, J., Ng, A. M. H., Selvi Naicker, A., Ismail, O. H. R., Athi Kumar, R. K., & Lokanathan, Y. (2020) · International Journal of Molecular Sciences · doi:10.3390/ijms21207533 · Cited by 1,270
Necroptosis and RIPK1-mediated neuroinflammation in CNS diseases
Yuan, J., Amin, P., & Ofengeim, D. (2019) · Nature Reviews Neuroscience · doi:10.1038/s41583-018-0093-1 · Cited by 967
Necroptosis: a regulated inflammatory mode of cell death
Dhuriya, Y. K., & Sharma, D. (2018) · Journal of Neuroinflammation · doi:10.1186/s12974-018-1235-0 · Cited by 718
Targeting RIPK1 for the treatment of human diseases
Degterev, A., Ofengeim, D., & Yuan, J. (2019) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1901179116 · Cited by 408
Deferoxamine promotes recovery of traumatic spinal cord injury by inhibiting ferroptosis
Feng, S.-Q., Yao, X., Zhang, Y., Hao, J., Duan, H.-Q., Zhao, C.-X., Sun, C., Li, B., Fan, B.-Y., Wang, X., et al. (2019) · Neural Regeneration Research · doi:10.4103/1673-5374.245480 · Cited by 298
Metadata
- Authors
- Fan, H., Zhang, K., Shan, L., Kuang, F., Chen, K., Zhu, K., Ma, H., Ju, G., & Wang, Y.-Z.
- Venue
- Molecular Neurodegeneration
- Published
- 2016-02-03
- Volume
- 11
- Issue
- 1
- Pages
- 14-14
- ISSN
- 1750-1326 (ISSN-L)
- OpenAlex
- W2255872712
- Cited by
- 223
- References
- 10
- FWCI
- 12.51
- Type
- journal article
- Language
- en
- Open access
- gold
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- Fan, H., Zhang, K., Shan, L., Kuang, F., Chen, K., Zhu, K., Ma, H., Ju, G., & Wang, Y.-Z. (2016). Reactive astrocytes undergo M1 microglia/macrohpages-induced necroptosis in spinal cord injury. Molecular Neurodegeneration. https://doi.org/10.1186/s13024-016-0081-8
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