Detection of Leishmania infantum DNA and antibodies against Anaplasma spp.; Borrelia burgdorferi s.l. and Ehrlichia canis in a dog kennel in South-Central Romania
Open accessAbstract
Canine vector-borne diseases are caused by pathogens transmitted by arthropods including ticks, mosquitoes and sand flies. Many canine vector-borne diseases are of zoonotic importance. This study aimed to assess the prevalence of vector-borne infections caused by Dirofilaria immitis, Ehrlichia canis, Borrelia burgdorferi sensu lato, Anaplasma spp. and Leishmania infantum in a dog kennel in Argeș County, Romania. Dog kennels are shelters for stray dogs with no officially registered owners that are gathered to be neutered and/or boarded for national/international adoptions by various public or private organizations. The international dog adoptions might represent a risk in the transmission of pathogens into new regions. In this context, a total number of 149 blood samples and 149 conjunctival swabs from asymptomatic kennel dogs were assessed using serology and quantitative real-time polymerase chain reaction. Antibodies against B. burgdorferi s.l. were detected in one dog (0.6%), anti-Anaplasma antibodies were found in five dogs (3.3%), while ten dogs (6.7%) tested positive for D. immitis antigen. Overall, 20.1% (30/149) of dogs were positive for L. infantum DNA. All samples were seronegative for anti-Leishmania antibodies. When adopting dogs from this region of Romania, owners should be aware of possible infection with especially L. infantum. The travel of infected dogs may introduce the infection to areas where leishmaniasis is not present.
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The highest canine heartworm prevalence in Romania was in Galati, southeast, with 42.2% of studied dogs infected.
“The highest prevalence was shown in Galati, in the southeast, 42.2% of the studied dogs being infected [78].”
From What has happened to heartworm disease in Europe in the last 10 years? (2022) · cites this paper as [78]
More optimal conditions created by temperature increases allow the spread of mosquitoes to other areas where previously climatic conditions prevented their presence.
“This has led to the creation of more optimal conditions that allow the spread of mosquitoes to other areas where previously the climatic conditions prevented their presence [1,55,78,166,167].”
From What has happened to heartworm disease in Europe in the last 10 years? (2022) · cites this paper as [78]
In Romania, a GIS study demonstrated high risk of D. immitis infection in the southeast and moderate risk in the centre of the country due to favourable temperature and humidity for culicid mosquitoes; these regions were characterised by floodplains and had the highest canine prevalence, corroborating the validity of the prediction model.
“A study of similar characteristics was carried out in Romania [78], where a high risk of infection in the southeast and moderate in the centre of the country were demonstrated, due to favourable temperature and humidity for the presence of culicid mosquitoes. These regions were characterised by floodplains; moreover, in these regions, the highest canine prevalence was found, corroborating the”
From What has happened to heartworm disease in Europe in the last 10 years? (2022) · cites this paper as [78]
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“None of the dogs in the present study tested positive for L. infantum , although competent sandflies have been identified in South-Eastern Romania (Cazan et al., 2021), and positive DNA samples were detected in a dog kennel in Arges County (Cazan et al., 2020).”
- Authors
- Cazan, C. D., Ionică, A. M., Matei, I. A., D’Amico, G., Muñoz, C., Berriatua, E., & Dumitrache, M. O.
- Affiliations
- University of Agricultural Sciences and Veterinary Medicine of Cluj-Napoca (RO)
- Universidad de Murcia (ES)
- Corresponding author
- Cazan, Cristina Daniela
- ORCID
- Venue
- Acta veterinaria Scandinavica
- Published
- 2020-08-03
- Volume
- 62
- Issue
- 1
- Pages
- 42-42
- ISSN
- 0044-605X (ISSN-L)
- 1751-0147
- PMC
- PMC7398206
- OpenAlex
- W3047241578
- Cited by
- 20
- References
- 25
- Citations by year
- 2026: 1 · 2025: 4 · 2024: 3 · 2023: 3 · 2022: 5 · 2021: 3 · 2020: 1
- FWCI
- 2.25
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Locations
- Acta veterinaria Scandinavica (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- Hispana (publishedVersion, cc-by)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Funders
- Unitatea Executiva pentru Finantarea Invatamantului Superior, a Cercetarii, Dezvoltarii si Inovarii (TE299/2015, PD38/2018)
- Topics
- Health Sciences › Medicine › Public Health, Environmental and Occupational Health › Research on Leishmaniasis Studies
- Life Sciences › Immunology and Microbiology › Parasitology › Vector-borne infectious diseases
- Health Sciences › Medicine › Infectious Diseases › Viral Infections and Vectors
- Keywords
- canine vector-borne diseases, Leishmania infantum, canine leishmaniasis, Ehrlichia canis, Borrelia burgdorferi sensu lato, Anaplasma, Dirofilaria immitis, stray dogs, dog kennels, zoonotic transmission, seroprevalence, parasite DNA detection, conjunctival swabs, Argeș County
- MeSH terms
- Anaplasma, Anaplasmosis, Animals, Antibodies, Bacterial, Disease Vectors, Dog Diseases, Dogs, Female, Leishmaniasis, Visceral, Lyme Disease, Male, Romania, Prevalence, DNA, Protozoan, Ehrlichiosis, Leishmania infantum, Borrelia burgdorferi, Ehrlichia canis
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