Photoautotrophic production of polyhydroxyalkanoates in a synthetic mixed culture of Synechococcus elongatus cscB and Pseudomonas putida cscAB
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One of the major challenges for the present and future generations is to find suitable substitutes for the fossil resources we rely on today. Cyanobacterial carbohydrates have been discussed as an emerging renewable feedstock in industrial biotechnology for the production of fuels and chemicals, showing promising production rates when compared to crop-based feedstock. However, intrinsic capacities of cyanobacteria to produce biotechnological compounds are limited and yields are low. Here, we present an approach to circumvent these problems by employing a synthetic bacterial co-culture for the carbon-neutral production of polyhydroxyalkanoates (PHAs) from CO 2 . The co-culture consists of two bio - modules : Bio - module I , in which the cyanobacterial strain Synechococcus elongatus cscB fixes CO 2 , converts it to sucrose, and exports it into the culture supernatant; and bio - module II , where this sugar serves as C-source for Pseudomonas putida cscAB and is converted to PHAs that are accumulated in the cytoplasm. By applying a nitrogen-limited process, we achieved a maximal PHA production rate of 23.8 mg/(L day) and a maximal titer of 156 mg/L. We will discuss the present shortcomings of the process and show the potential for future improvement. These results demonstrate the feasibility of mixed cultures of S. elongatus cscB and P. putida cscAB for PHA production, making room for the cornucopia of possible products that are described for P. putida . The construction of more efficient sucrose-utilizing P. putida phenotypes and the optimization of process conditions will increase yields and productivities and eventually close the gap in the contemporary process. In the long term, the co-culture may serve as a platform process, in which P. putida is used as a chassis for the implementation of synthetic metabolic pathways for biotechnological production of value-added products.
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Löwe et al. demonstrated a two-module design pairing S. elongatus CscB with Pseudomonas putida for photoautotrophic PHA production from CO2.
“Löwe et al. demonstrated a similar two-module design pairing S. elongatus CscB with Pseudomonas putida for photoautotrophic PHA production from CO2 [194].”
From Cyanobacteria as a Photosynthetic Chassis for Metabolic Pathway Engineering with Heterologous Gene Expression (2026) · cites this paper as [194]
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- Authors
- Löwe, H., Hobmeier, K., Moos, M., Kremling, A., & Pflüger-Grau, K.
- Affiliations
- Technical University of Munich (DE)
- Venue
- Biotechnology for Biofuels
- Published
- 2017-07-19
- Volume
- 10
- Issue
- 1
- Pages
- 190-190
- ISSN
- 1754-6834 (ISSN-L)
- PMC
- PMC5517840
- OpenAlex
- W2736712864
- Cited by
- 114
- References
- 35
- Citations by year
- 2026: 6 · 2025: 10 · 2024: 13 · 2023: 15 · 2022: 20 · 2021: 18 · 2020: 21 · 2019: 3 · 2018: 7 · 2017: 1
- FWCI
- 3.15
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- cc-by
- Locations
- Biotechnology for Biofuels (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- mediaTUM – the media and publications repository of the Technical University Munich (Technical University Munich) (submittedVersion, other-oa)
- Funders
- U.S. Department of Defense
- Technische Universität München
- Centro Nacional de Biotecnología
- Topics
- Physical Sciences › Materials Science › Biomaterials › biodegradable polymer synthesis and properties
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Microbial Metabolic Engineering and Bioproduction
- Physical Sciences › Energy › Renewable Energy, Sustainability and the Environment › Algal biology and biofuel production
- Keywords
- polyhydroxyalkanoates, sucrose export, renewable raw materials, biopolymer production, chassis strain, synthetic metabolic pathways
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