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Effects of acids pre-treatment on the microbial fermentation process for bioethanol production from microalgae
Open accessAbstract
Microalgae are one of the promising feedstock that consists of high carbohydrate content which can be converted into bioethanol. Pre-treatment is one of the critical steps required to release fermentable sugars to be used in the microbial fermentation process. In this study, the reducing sugar concentration of Chlorella species was investigated by pre-treating the biomass with dilute sulfuric acid and acetic acid at different concentrations 1%, 3%, 5%, 7%, and 9% (v/v). 3,5-Dinitrosalicylic acid (DNS) method, FTIR, and GC-FID were employed to evaluate the reducing sugar concentration, functional groups of alcohol bonds and concentration of bioethanol, respectively. The two-way ANOVA results ( p < 0.05) indicated that there was a significant difference in the concentration and type of acids towards bioethanol production. The highest bioethanol yield obtained was 0.28 g ethanol/g microalgae which was found in microalgae sample pre-treated with 5% (v/v) sulfuric acid while 0.23 g ethanol/g microalgal biomass was presented in microalgae sample pre-treated with 5% (v/v) acetic acid. The application of acid pre-treatment on microalgae for bioethanol production will contribute to higher effectiveness and lower energy consumption compared to other pre-treatment methods. The findings from this study are essential for the commercial production of bioethanol from microalgae.
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“It also degrades polysaccharides, lowers energy consumption, and increases sugar yields, making it a cost-efficient option compared to other pretreatment technologies [197].”
From A Review of Algae-Based Carbon Capture, Utilization, and Storage (Algae-Based CCUS) (2024) · cites this paper as [197]
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Cites
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Cited by
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Kumar, M., Sun, Y., Rathour, R., Pandey, A., Thakur, I. S., & Tsang, D. C. W. (2020) · Science of The Total Environment · doi:10.1016/j.scitotenv.2020.137116 · Cited by 472
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Self-citationBiofuel production from microalgae: challenges and chances
Hoang, A. T., Sirohi, R., Pandey, A., Nižetić, S., Lam, S. S., Chen, W., Luque, R., Thomas, S., Arıcı, M., & Pham, V. V. (2022) · Phytochemistry Reviews · doi:10.1007/s11101-022-09819-y · Cited by 296
“On the other hand, microalgae that have higher carbohydrate content are more suitable for the production of fermentative alcohol (Phwan et al. 2019).”
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Bhatt, P., Bhandari, G., Turco, R. F., Aminikhoei, Z., Bhatt, K., & Simsek, H. (2022) · Environmental Pollution · doi:10.1016/j.envpol.2022.119688 · Cited by 148
Metadata
- Authors
- Phwan, C. K., Chew, K. W., Sebayang, A. H., Ong, H. C., Ling, T. C., Malek, M. A., Ho, Y.-C., & Show, P. L.
- Affiliations
- University of Malaya (MY)
- University of Nottingham (GB)
- Universiti Teknologi Petronas (MY)
- Corresponding author
- Show, Pau Loke
- Venue
- Biotechnology for Biofuels
- Published
- 2019-07-31
- Volume
- 12
- Issue
- 1
- Pages
- 191-191
- ISSN
- 1754-6834 (ISSN-L)
- PMC
- PMC6668136
- OpenAlex
- W2966314663
- Cited by
- 143
- References
- 29
- Citations by year
- 2026: 14 · 2025: 21 · 2024: 18 · 2023: 22 · 2022: 27 · 2021: 22 · 2020: 18 · 2019: 1
- FWCI
- 5.99
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Locations
- Biotechnology for Biofuels (publishedVersion, cc-by)
- Europe PMC (PubMed Central) (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- The University of Malaya Research Repository (University of Malaya) (submittedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- UTS ePRESS (University of Technology Sydney) (submittedVersion, other-oa)
- Funders
- Fundamental Research Grant Scheme (FRGS/1/2015/SG05/UNIM/03/1)
- iRMC Bold 2025, Universiti Tenaga Nasional (RJO 1043 6494)
- Topics
- Physical Sciences › Engineering › Biomedical Engineering › Biofuel production and bioconversion
- Physical Sciences › Energy › Renewable Energy, Sustainability and the Environment › Algal biology and biofuel production
- Physical Sciences › Energy › Renewable Energy, Sustainability and the Environment › Anaerobic Digestion and Biogas Production
- Keywords
- microalgae, bioethanol production, acid pretreatment, sulfuric acid pretreatment, acetic acid pretreatment, Chlorella, reducing sugars, fermentable sugars, microbial fermentation, ethanol yield
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