Oxidative Status Predicts Quality in Human Mesenchymal Stem Cells
Open accessAbstract
BACKGROUND: Human bone marrow-derived mesenchymal stem cells (MSC) are adult progenitor cells with great potential for application in cell-based therapies. From a cell-based therapy perspective, there are two limitations to MSC use: (1) these therapies require large numbers of cells, and long-term expansion of MSC in vitro promotes replicative senescence; and (2) patient variability is a challenge for defining MSC quality standards for transplantation. This study aimed to determine whether low or high oxidative status of MSC correlate with changes in cell expansion and differentiation potentials. METHODS: We investigated functional aspects of mitochondria, such as cell metabolic activity indicators and expression of antioxidant enzymes. Furthermore, we tested if senescence-induced changes in oxidative status of MSC could be counteracted by methylene blue (MB), an alternative mitochondrial electron transfer known to enhance cell bioenergetics. RESULTS: MSC isolated from donors of the same age showed distinctive behavior in culture and were grouped as weak (low colony-forming units (CFU) and a short life in vitro) and vigorous MSC (high CFU and a long life in vitro). In comparison to weak MSC, vigorous MSC had oxidative status characterized by lower mitochondrial membrane potential, lower mitochondrial activity, and fewer reactive oxygen species production, as well as reduced mitochondrial biogenesis. Vigorous MSC had a significantly higher expansion potential compared to weak MSC, while no differences were observed during differentiation. MB treatment significantly improved expansion and differentiation potential, however only in vigorous MSC. CONCLUSIONS: Together, these results demonstrate the importance of mitochondrial function in MSC in vitro, and that cells with low oxidative status levels are better candidates for cell-based therapies.
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Methylene blue may also prevent senescence and OS in primary retinal ganglion cells and mesenchymal stem cells, but its efficacy on stem cells remains limited to the cellular fraction characterized by a lower baseline level of OS.
“Besides skin aging, MB may also prevent senescence and OS in other cell types, such as primary retinal ganglion cells and mesenchymal stem cells (MSCs), but its efficacy on stem cells remains limited to the cellular fraction characterized by a lower baseline level of OS [81,82].”
From The Role of Antioxidants in the Interplay between Oxidative Stress and Senescence (2022) · cites this paper as [81]
Antioxidants efficacy might depend upon oxidative stress levels.
“Besides the dosage, a careful evaluation of the optimal administration window can be also crucial to achieve a clinical benefit as antioxidants efficacy might depend upon OS levels [81].”
From The Role of Antioxidants in the Interplay between Oxidative Stress and Senescence (2022) · cites this paper as [81]
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López-Otín, C., Blasco, M. A., Partridge, L., Serrano, M., & Kroemer, G. (2013) · Cell · doi:10.1016/j.cell.2013.05.039 · Cited by 14,982 · see citing works
Bone marrow cells regenerate infarcted myocardium
Orlic, D., Kajstura, J., Chimenti, S., Jakoniuk, I., Anderson, S. M., Li, B., Pickel, J. M., McKay, R. D. G., Nadal‐Ginard, B., Bodine, D. M., et al. (2001) · Nature · doi:10.1038/35070587 · Cited by 5,149 · see citing works
Marrow Stromal Cells as Stem Cells for Nonhematopoietic Tissues
Prockop, D. J. (1997) · Science · doi:10.1126/science.276.5309.71 · Cited by 4,629 · see citing works
Mitochondrial diseases in man and mouse
Wallace, D. C. (1999) · Science · doi:10.1126/science.283.5407.1482 · Cited by 3,060 · see citing works
Mitochondrial free radical generation, oxidative stress, and aging
Cadenas, E., & Davies, K. J. A. (2000) · Free Radical Biology and Medicine · doi:10.1016/s0891-5849(00)00317-8 · Cited by 3,010 · see citing works
Oxidative damage and mitochondrial decay in aging.
Shigenaga, M. K., Hagen, T. M., & Ames, B. N. (1994) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.91.23.10771 · Cited by 2,055 · see citing works
Debacq-Chainiaux, F., Erusalimsky, J. D., Campisi, J., & Toussaint, O. (2009) · Nature Protocols · doi:10.1038/nprot.2009.191 · Cited by 1,668 · see citing works
Zhang, H., Ryu, D., Wu, Y., Gariani, K., Wang, X., Luan, P., D’Amico, D., Ropelle, E. R., Lutolf, M. P., Aebersold, R., et al. (2016) · Science · doi:10.1126/science.aaf2693 · Cited by 1,255 · see citing works
Mouchiroud, L., Houtkooper, R. H., Moullan, N., Katsyuba, E., Ryu, D., Cantó, C., Mottis, A., Jo, Y.-S., Viswanathan, M., Schoonjans, K., et al. (2013) · Cell · doi:10.1016/j.cell.2013.06.016 · Cited by 1,210 · see citing works
Calorie restriction extends Saccharomyces cerevisiae lifespan by increasing respiration
Lin, S.-J., Kaeberlein, M., Andalis, A. A., Sturtz, L. A., Defossez, P.-A., Culotta, V. C., Fink, G. R., & Guarente, L. (2002) · Nature · doi:10.1038/nature00829 · Cited by 1,057 · see citing works
Mitonuclear protein imbalance as a conserved longevity mechanism
Houtkooper, R. H., Mouchiroud, L., Ryu, D., Moullan, N., Katsyuba, E., Knott, G., Williams, R. W., & Auwerx, J. (2013) · Nature · doi:10.1038/nature12188 · Cited by 1,035 · see citing works
Rapid expansion of recycling stem cells in cultures of plastic-adherent cells from human bone marrow
Colter, D. C., Class, R., DiGirolamo, C. M., & Prockop, D. J. (2000) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.97.7.3213 · Cited by 1,019 · see citing works
Uncoupling to survive? The role of mitochondrial inefficiency in ageing
Brand, M. D. (2000) · Experimental Gerontology · doi:10.1016/s0531-5565(00)00135-2 · Cited by 789 · see citing works
The mitochondrial UPR – protecting organelle protein homeostasis
Haynes, C. M., & Ron, D. (2010) · Journal of Cell Science · doi:10.1242/jcs.075119 · Cited by 490 · see citing works
Terman, A., Kurz, T., Navratil, M., Arriaga, E. A., & Brunk, U. T. (2010) · Antioxidants & Redox Signaling · doi:10.1089/ars.2009.2598 · Cited by 490 · see citing works
A quantitative PCR method for measuring absolute telomere length
O’Callaghan, N. J., & Fenech, M. F. (2011) · Biological Procedures Online · doi:10.1186/1480-9222-13-3 · Cited by 490 · see citing works
Muscle Mitohormesis Promotes Longevity via Systemic Repression of Insulin Signaling
Owusu-Ansah, E., Song, W., & Perrimon, N. (2013) · Cell · doi:10.1016/j.cell.2013.09.021 · Cited by 395 · see citing works
Mitochondrial Membrane Potential Identifies Cells with Enhanced Stemness for Cellular Therapy
Sukumar, M., Liu, J., Mehta, G. U., Patel, S. J., Roychoudhuri, R., Crompton, J. G., Klebanoff, C. A., Ji, Y., Li, P., Yu, Z., et al. (2015) · Cell Metabolism · doi:10.1016/j.cmet.2015.11.002 · Cited by 391 · see citing works
Number of osteoprogenitor cells in human bone marrow markedly decreases after skeletal maturation
Nishida, S., Endo, N., Yamagiwa, H., Tanizawa, T., & Takahashi, H. E. (1999) · Journal of Bone and Mineral Metabolism · doi:10.1007/s007740050081 · Cited by 303 · see citing works
Methylene Blue Delays Cellular Senescence and Enhances Key Mitochondrial Biochemical Pathways
Atamna, H., Nguyen, A., Schultz, C., Boyle, K., Newberry, J., Kato, H., & Ames, B. N. (2008) · The FASEB Journal · doi:10.1096/fj.07-9610com · Cited by 272 · see citing works
Regulation of mitochondrial fusion and division
Cerveny, K. L., Tamura, Y., Zhang, Z., Jensen, R. E., & Sesaki, H. (2007) · Trends in Cell Biology · doi:10.1016/j.tcb.2007.08.006 · Cited by 261 · see citing works
Bonyadi, M. J., Waldman, S. D., Liu, D., Aubin, J. E., Grynpas, M. D., & Stanford, W. L. (2003) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1036475100 · Cited by 247 · see citing works
Simultaneous Preparation and Quantitation of Proteoglycans by Precipitation with Alcian Blue
Bjornsson, S. (1993) · Analytical Biochemistry · doi:10.1006/abio.1993.1197 · Cited by 239 · see citing works
Stromal Mechanisms of Bone Marrow: Cloning in Vitro and Retransplantation in Vivo
Friedenstein, A. J. (1980) · Hämatologie und Bluttransfusion · doi:10.1007/978-3-642-67319-1_3 · Cited by 226 · see citing works
NAD <sup>+</sup> metabolism: A therapeutic target for age-related metabolic disease
Mouchiroud, L., Houtkooper, R. H., & Auwerx, J. (2013) · Critical Reviews in Biochemistry and Molecular Biology · doi:10.3109/10409238.2013.789479 · Cited by 193 · see citing works
Prophylaxis and reversal of ifosfamide encephalopathy with methylene-blue
Kupfer, A., Aeschlimann, C., Wermuth, B., & Cerny, T. (1994) · The Lancet · doi:10.1016/s0140-6736(94)91839-2 · Cited by 178 · see citing works
Mitochondrial Proteostasis in the Control of Aging and Longevity
Jensen, M. B., & Jasper, H. (2014) · Cell Metabolism · doi:10.1016/j.cmet.2014.05.006 · Cited by 164 · see citing works
Skeletal Progenitor Cells and Ageing Human Populations
Oreffo, R. O. C., Bord, S., & Triffitt, J. T. (1998) · Clinical Science · doi:10.1042/cs0940549 · Cited by 164 · see citing works
Valcarcel‐Ares, M. N., Gautam, T., Warrington, J. P., Bailey‐Downs, L. C., Sosnowska, D., de Cabo, R., Losonczy, G., Sonntag, W. E., Ungvari, Z. I., & Csiszár, A. (2012) · The Journals of Gerontology Series A · doi:10.1093/gerona/glr229 · Cited by 139 · see citing works
Protective Role of Methylene Blue in Alzheimer's Disease via Mitochondria and Cytochrome c Oxidase
Atamna, H., & Kumar, R. (2010) · Journal of Alzheimer s Disease · doi:10.3233/jad-2010-100414 · Cited by 137 · see citing works
THE CONTROL OF METHEMOGLOBINEMIA WITH METHYLENE BLUE 12
Wendel, W. B. (1939) · Journal of Clinical Investigation · doi:10.1172/jci101033 · Cited by 120 · see citing works
Methylene blue alleviates nuclear and mitochondrial abnormalities in progeria
Xiong, Z., Choi, J. Y., Wang, K., Zhang, H., Tariq, Z., Wu, D., Ko, E., LaDana, C., Sesaki, H., & Cao, K. (2015) · Aging Cell · doi:10.1111/acel.12434 · Cited by 118 · see citing works
Telomere length varies by DNA extraction method: implications for epidemiologic research
Cunningham, J. M., Johnson, R. A., Litzelman, K., Skinner, H. G., Seo, S., Engelman, C. D., Vanderboom, R. J., Kimmel, G. W., Gangnon, R. E., Riegert-Johnson, D. L., et al. (2013) · Cancer Epidemiology, Biomarkers & Prevention · doi:10.1158/1055-9965.epi-13-0409 · Cited by 111 · see citing works
Reversing the Warburg Effect as a Treatment for Glioblastoma
Poteet, E. C., Choudhury, G. R., Winters, A., Li, W., Ryou, M., Liu, R., Tang, L., Ghorpade, A., Wen, Y., Yuan, F., et al. (2013) · Journal of Biological Chemistry · doi:10.1074/jbc.m112.440354 · Cited by 111 · see citing works
Kuznetsov, S. A., Mankani, M. H., Bianco, P., & Robey, P. G. (2008) · Stem Cell Research · doi:10.1016/j.scr.2008.07.007 · Cited by 96 · see citing works
Wang, Z., Wang, Y., Ye, J., Lu, X., Cheng, Y. H., Xiang, L., Chen, L., Feng, W., Shi, H., Yu, X., et al. (2014) · Journal of Cellular and Molecular Medicine · doi:10.1111/jcmm.12346 · Cited by 94 · see citing works
Sgarbi, G., Matarrese, P., Pinti, M., Lanzarini, C., Ascione, B., Gibellini, L., Dika, E., Patrizi, A., Tommasino, C., Capri, M., et al. (2014) · Aging · doi:10.18632/aging.100654 · Cited by 90 · see citing works
Kelner, M. J., Bagnell, R. D., Hale, B., & Alexander, N. M. (1988) · Archives of Biochemistry and Biophysics · doi:10.1016/0003-9861(88)90393-1 · Cited by 77 · see citing works
Bertolo, A., Mehr, M., Janner-Jametti, T., Graumann, U., Aebli, N., Baur, M., Ferguson, S. J., & Stoyanov, J. V. (2013) · Journal of Tissue Engineering and Regenerative Medicine · doi:10.1002/term.1734 · Cited by 68 · see citing works
Self-citationBertolo, A., Mehr, M., Aebli, N., Baur, M., Ferguson, S. J., & Stoyanov, J. V. (2011) · European Spine Journal · doi:10.1007/s00586-011-1975-3 · Cited by 66 · see citing works
Self-citationFactors affecting mesenchymal stromal cells yield from bone marrow aspiration
Li, J., Wong, W. H. S., Chan, S., Chim, C. S., Cheung, K., Lee, T.-L., Au, W., Ha, S., Lie, A. K.-W., Lau, Y., et al. (2011) · Chinese Journal of Cancer Research · doi:10.1007/s11670-011-0043-1 · Cited by 48 · see citing works
Sevini, F., Giuliani, C., Vianello, D., Giampieri, E., Santoro, A., Biondi, F., Garagnani, P., Passarino, G., Luiselli, D., Capri, M., et al. (2014) · Experimental Gerontology · doi:10.1016/j.exger.2014.03.022 · Cited by 46 · see citing works
Reproducible Quantitative PCR of Mitochondrial and Nuclear DNA Copy Number Using the LightCycler™
Wong, A., & Cortopassi, G. A. (2003) · Mitochondrial DNA · doi:10.1385/1-59259-284-8:129 · Cited by 43 · see citing works
A Sensitive Method to Quantify Senescent Cancer Cells
Cahu, J., & Sola, B. (2013) · Journal of Visualized Experiments · doi:10.3791/50494 · Cited by 41 · see citing works
Hofer, E. L., Labovsky, V., Russa, V. L., Vallone, V. B. F., Honegger, A. E., Belloc, C. G., Wen, H. C., Bordenave, R. H., Bullorsky, E. O., Feldman, L. J., et al. (2009) · Stem Cells and Development · doi:10.1089/scd.2008.0375 · Cited by 24 · see citing works
Alston, T. A. (2014) · Journal of Clinical Anesthesia · doi:10.1016/j.jclinane.2014.09.001 · Cited by 5 · see citing works
Flagged first, then by citations
Cited by
Sorted by citations
Zhang, H., Xu, R., Li, B., Xin, Z., Ling, Z., Zhu, W., Li, X., Zhang, P., Fu, Y., Chen, J., et al. (2021) · Cell Death and Differentiation · doi:10.1038/s41418-021-00858-0 · Cited by 154 · see citing works
The Role of Antioxidants in the Interplay between Oxidative Stress and Senescence
Varesi, A., Chirumbolo, S., Campagnoli, L. I. M., Pierella, E., Piccini, G. B., Carrara, A., Ricevuti, G., Scassellati, C., Bonvicini, C., & Pascale, A. (2022) · Antioxidants · doi:10.3390/antiox11071224 · Cited by 145 · see citing works
Molecular Mechanisms Contributing to Mesenchymal Stromal Cell Aging
Neri, S., & Borzı̀, R. M. (2020) · Biomolecules · doi:10.3390/biom10020340 · Cited by 122 · see citing works
Bertolo, A., Baur, M., Guerrero, J., Pötzel, T., & Stoyanov, J. V. (2019) · Scientific Reports · doi:10.1038/s41598-019-38546-2 · Cited by 115 · see citing works
Self-citationMetabolic reprograming of anti-tumor immunity
Sukumar, M., Kishton, R. J., & Restifo, N. P. (2017) · Current Opinion in Immunology · doi:10.1016/j.coi.2017.03.011 · Cited by 100 · see citing works
Lineage Commitment, Signaling Pathways, and the Cytoskeleton Systems in Mesenchymal Stem Cells
Saidova, A. A., & Vorobjev, I. A. (2019) · Tissue Engineering Part B Reviews · doi:10.1089/ten.teb.2019.0250 · Cited by 99 · see citing works
Mesenchymal stem cell-mediated transfer of mitochondria: mechanisms and functional impact
Velarde, F., Ezquerra, S., Delbruyere, X., Caicedo, A., Hidalgo, Y., & Khoury, M. (2022) · Cellular and Molecular Life Sciences · doi:10.1007/s00018-022-04207-3 · Cited by 99 · see citing works
Antioxidant Structure–Activity Relationship Analysis of Five Dihydrochalcones
Li, X., Chen, B., Xie, H., He, Y., Zhong, D., & Chen, D. (2018) · Molecules · doi:10.3390/molecules23051162 · Cited by 98 · see citing works
Mesenchymal Stem Cells on Horizon: A New Arsenal of Therapeutic Agents
Abbasi‐Malati, Z., Roushandeh, A. M., Kuwahara, Y., & Roudkenar, M. H. (2018) · Stem Cell Reviews and Reports · doi:10.1007/s12015-018-9817-x · Cited by 85 · see citing works
Mancini, Ö. K., Lora, M., Cuillerier, A., Tim, D. S., Hamdy, R. C., Burelle, Y., Servant, M. J., Stochaj, U., & Colmegna, I. (2017) · Circulation Research · doi:10.1161/circresaha.117.311400 · Cited by 54 · see citing works
Characterization of Senescence of Human Adipose-Derived Stem Cells After Long-Term Expansion
Truong, N. C., Bui, K. H.-T., & Pham, P. V. (2018) · Advances in experimental medicine and biology · doi:10.1007/5584_2018_235 · Cited by 49 · see citing works
Li, X., Xie, Y., Li, K., Wu, A., Xie, H., Guo, Q., Xue, P., Maleshibek, Y., Zhao, W., Guo, J., et al. (2018) · Molecules · doi:10.3390/molecules23020498 · Cited by 38 · see citing works
Kok, Z. Y., Alaidaroos, N. Y. A., Alraies, A., Colombo, J. S., Davies, L. C., Waddington, R. J., Sloan, A. J., & Moseley, R. (2022) · Stem Cells International · doi:10.1155/2022/9127074 · Cited by 33 · see citing works
Nuclear Nox4 interaction with prelamin A is associated with nuclear redox control of stem cell aging
Casciaro, F., Beretti, F., Zavatti, M., McCubrey, J. A., Ratti, S., Marmiroli, S., Follo, M. Y., & Maraldi, T. (2018) · Aging · doi:10.18632/aging.101599 · Cited by 30 · see citing works
Sahibdad, I., Khalid, S., Chaudhry, G. R., Salim, A., Begum, S., & Khan, I. (2023) · World Journal of Stem Cells · doi:10.4252/wjsc.v15.i7.751 · Cited by 22 · see citing works
Mitochondrial adaptation in human mesenchymal stem cells following ionizing radiation
Patten, D. A., Ouellet, M., Allan, D., Germain, M., Baird, S. D., Harper, M., & Richardson, R. B. (2019) · The FASEB Journal · doi:10.1096/fj.201801483rr · Cited by 19 · see citing works
Castro, B., Martinez-Redondo, D., Gartzia, I., Alonso‐Varona, A., Garrido, P., & Palomares, T. (2019) · Journal of Tissue Engineering and Regenerative Medicine · doi:10.1002/term.2797 · Cited by 19 · see citing works
Alaidaroos, N. Y. A., Alraies, A., Waddington, R. J., Sloan, A. J., & Moseley, R. (2021) · Stem Cell Research & Therapy · doi:10.1186/s13287-021-02209-9 · Cited by 19 · see citing works
Rapid Detection of Senescent Mesenchymal Stromal Cells by a Fluorescent Probe
Ang, J., Lee, Y., Raghothaman, D., Jayaraman, P., Teo, K. L., Khan, F. J., Reuveny, S., Chang, Y., Kang, N., & Oh, S. (2019) · Biotechnology Journal · doi:10.1002/biot.201800691 · Cited by 17 · see citing works
Marcoccia, R., Nesci, S., Merlo, B., Ballotta, G., Algieri, C., Pagliarani, A., & Iacono, E. (2021) · PLoS ONE · doi:10.1371/journal.pone.0247567 · Cited by 15 · see citing works
Hazra, S., Li, R., Vamesu, B. M., Jilling, T., Ballinger, S. W., Ambalavanan, N., & Kandasamy, J. (2022) · Scientific Reports · doi:10.1038/s41598-022-22478-5 · Cited by 13 · see citing works
Zhang, B., Ye, H., & Yang, A. (2018) · BMC Systems Biology · doi:10.1186/s12918-018-0560-3 · Cited by 10 · see citing works
Nguyen, H., Hsu, C.-C., Meeson, A., Oldershaw, R., Richardson, G., Czosseck, A., & Lundy, D. J. (2024) · Stem Cells and Development · doi:10.1089/scd.2024.0103 · Cited by 5 · see citing works
Kadhim, M. F., Arbi, M. E., Samawi, F. T., & Gargouri, A. F. (2025) · Genes · doi:10.3390/genes16121402 · Cited by 2 · see citing works
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Kitaeva, K. V., Kiyasov, A. P., Rizvanov, A. A., Rutland, C. S., & Solovyeva, V. V. (2022) · Elsevier eBooks · doi:10.1016/b978-0-12-823761-8.00007-0 · Cited by 1 · see citing works
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Bartosz, G., Pieńkowska, N., Kut, K., Cieniek, B., Stefaniuk, I., & Sadowska‐Bartosz, I. (2023) · International Journal of Molecular Sciences · doi:10.3390/ijms242316675 · Cited by 1 · see citing works
Madkour, M. I., Hassan, R. E., Sherif, N. M., Awadallah, S. M., Farahat, N. M., Abdelrahim, D. N., Alhasan, F., Taneera, J., & Faris, M. E. (2024) · Frontiers in Nutrition · doi:10.3389/fnut.2024.1409344 · Cited by 2 · see citing works
Kamaldinov, T., & Hahn, M. S. (2020) · Bioelectricity · doi:10.1089/bioe.2020.0006
Wang, D., Chen, W., Chang, Y., Yao, D., Liu, G., Huang, R., Fang, C., Zhou, C., Jia, L., Zhang, Z., et al. (2023) · Research Square · doi:10.21203/rs.3.rs-3497409/v1
Alaidaroos, N. Y. A., Alraies, A., Waddington, R. J., Sloan, A. J., & Moseley, R. (2021) · Figshare · doi:10.6084/m9.figshare.14053068
Alaidaroos, N. Y. A., Alraies, A., Waddington, R. J., Sloan, A. J., & Moseley, R. (2021) · Figshare · doi:10.6084/m9.figshare.14053074
Alaidaroos, N. Y. A., Alraies, A., Waddington, R. J., Sloan, A. J., & Moseley, R. (2021) · Figshare · doi:10.6084/m9.figshare.14053062
Alaidaroos, N. Y. A., Alraies, A., Waddington, R. J., Sloan, A. J., & Moseley, R. (2021) · Figshare · doi:10.6084/m9.figshare.14053077
Alaidaroos, N. Y. A., Alraies, A., Waddington, R. J., Sloan, A. J., & Moseley, R. (2021) · Figshare · doi:10.6084/m9.figshare.14053071
Capone, A., Merlo, B., Arshaghi, T. E., Barry, F., Bandioli, G., & Iacono, E. (2026) · SSRN Electronic Journal · doi:10.2139/ssrn.7513487
- Authors
- Bertolo, A., Capossela, S., Fränkl, G., Baur, M., Pötzel, T., & Stoyanov, J.
- Affiliations
- Swiss Paraplegic Research (CH)
- Swiss Paraplegic Center (CH)
- Luzerner Kantonsspital (CH)
- University of Bern (CH)
- Corresponding author
- Stoyanov, Jivko
- Venue
- Stem Cell Research & Therapy
- Published
- 2017-01-06
- Volume
- 8
- Issue
- 1
- Pages
- 3-3
- ISSN
- 1757-6512 (ISSN-L)
- PMC
- PMC5217567
- OpenAlex
- W2569783565
- Cited by
- 36
- References
- 46
- Citations by year
- 2026: 1 · 2025: 1 · 2024: 2 · 2023: 3 · 2022: 5 · 2021: 8 · 2020: 2 · 2019: 5 · 2018: 6 · 2017: 3
- FWCI
- 2.51
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- cc-by
- Locations
- Stem Cell Research & Therapy (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- Funders
- Schweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschung (CR32I3_140717)
- Topics
- Health Sciences › Medicine › Genetics › Mesenchymal stem cell research
- Health Sciences › Medicine › Physiology › Telomeres, Telomerase, and Senescence
- Health Sciences › Medicine › Hematology › Hematopoietic Stem Cell Transplantation
- Keywords
- mesenchymal stem cells, oxidative status, replicative senescence, mitochondrial function, methylene blue, mitochondrial biogenesis, reactive oxygen species, mitochondrial membrane potential, differentiation potential, cell therapy, donor variability, antioxidant enzymes, colony-forming units
- MeSH terms
- Aged, Cell Differentiation, Cell Division, Cells, Cultured, Flow Cytometry, Humans, Male, Methylene Blue, Mitochondria, Oxidation-Reduction, Superoxides, Biomarkers, Cell Proliferation, Mesenchymal Stem Cells
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Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
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