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Cited by 23Plant tissue culture and regenerationPlant Genetic and Mutation StudiesEpigenetics and DNA Methylation

Barley somatic embryogenesis-an attempt to modify variation induced in tissue culture

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Orłowska, Renata·2021·Journal of Biological Research-Thessaloniki
DOI 10.1186/s40709-021-00138-5PMC PMC7962293
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Abstract

Background: Somatic embryogenesis is a phenomenon carried out in an environment that generates abiotic stress. Thus, regenerants may differ from the source of explants at the morphological, genetic, and epigenetic levels. The DNA changes may be the outcome of induction media ingredients (i.e., copper and silver ions) and their concentrations and time of in vitro cultures. Results: This study optimised the level of copper and silver ion concentration in culture media parallel with the induction medium longevity step towards obtaining barley regenerants via somatic embryogenesis with a minimum or maximum level of tissue culture-induced differences between the donor plant and its regenerants. The optimisation process is based on tissue culture-induced variation evaluated via the metAFLP approach for regenerants derived under varying in vitro tissue culture conditions and exploited by the Taguchi method. In the optimisation and verification experiments, various copper and silver ion concentrations and the different number of days differentiated the tested trials concerning the tissue culture-induced variation level, DNA demethylation, and de novo methylation, including symmetric (CG, CHG) and asymmetric (CHH) DNA sequence contexts. Verification of optimised conditions towards obtaining regenerants with minimum and maximum variability compared to donor plants proved useful. The main changes that discriminate optimised conditions belonged to DNA demethylation events with particular stress on CHG context. Conclusions: The combination of tissue culture-induced variation evaluated for eight experimental trials and implementation of the Taguchi method allowed the optimisation of the in vitro tissue culture conditions towards the minimum and maximum differences between a source of tissue explants (donor plant) and its regenerants from somatic embryos. The tissue culture-induced variation characteristic is mostly affected by demethylation with preferences towards CHG sequence context.

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Authors
Orłowska, R.
Affiliations
Plant Breeding and Acclimatization Institute - National Research Institute (PL)
Corresponding author
Orłowska, Renata
ORCID
Orłowska, Renata
Venue
Journal of Biological Research-Thessaloniki
Publisher
Springer Science and Business Media LLC
Published
2021-03-16
Volume
28
Issue
1
Pages
9-9
ISSN
  • 1790-045X (ISSN-L)
  • 2241-5793
DOI
10.1186/s40709-021-00138-5
PMC
PMC7962293
OpenAlex
W3137158420
Cited by
23
References
3
Citations by year
2026: 4 · 2025: 5 · 2022: 10 · 2021: 4
FWCI
1.10
Type
journal article
Language
en
Open access
Licence
Locations
  • Journal of Biological Research - Thessaloniki (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
Funders
Ministry of Agriculture and Rural Development (HORhn-801-PB-22/15-18)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Plant tissue culture and regeneration
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Genetic and Mutation Studies
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Epigenetics and DNA Methylation
Keywords
barley, somatic embryogenesis, copper ions, silver ions, culture medium optimization, DNA methylation, DNA demethylation, de novo methylation, Taguchi method, epigenetic variation

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