Cited by 59Plant tissue culture and regenerationEpigenetics and DNA MethylationPlant Genetic and Mutation Studies

Tissue Culture-Induced Genetic and Epigenetic Variation in Triticale (× Triticosecale Spp. Wittmack Ex A. Camus 1927) Regenerants

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Machczyńska, Joanna, Zimny, Janusz, Bednarek, Piotr Tomasz·2015·Plant Molecular Biology

Abstract

Plant regeneration via in vitro culture can induce genetic and epigenetic variation; however, the extent of such changes in triticale is not yet understood. In the present study, metAFLP, a variation of methylation-sensitive amplified fragment length polymorphism analysis, was used to investigate tissue culture-induced variation in triticale regenerants derived from four distinct genotypes using androgenesis and somatic embryogenesis. The metAFLP technique enabled identification of both sequence and DNA methylation pattern changes in a single experiment. Moreover, it was possible to quantify subtle effects such as sequence variation, demethylation, and de novo methylation, which affected 19, 5.5, 4.5% of sites, respectively. Comparison of variation in different genotypes and with different in vitro regeneration approaches demonstrated that both the culture technique and genetic background of donor plants affected tissue culture-induced variation. The results showed that the metAFLP approach could be used for quantification of tissue culture-induced variation and provided direct evidence that in vitro plant regeneration could cause genetic and epigenetic variation.

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  • In X Triticosecale spp., 51% of tissue culture-induced variation was observed.

    “51% of tissue culture-induced variation”

    From Somaclonal Variation and Clonal Fidelity in Commercial Micropropagation: Challenges and Perspectives (2025) · cites this paper as [156]

    Results > MetAFLP results, Table 2 (page not provided), reports mean total tissue culture-induced variation of 29.12% across triticale genotypes and regeneration approaches. Its highest listed value is 36.55%, not 51%. (LLM, conf=0.88)

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  1. Olhoft PM, Phillips RL (1994) DNA methylation and tissue culture-induced variation in mayze. Conference material (Plant Genome II Conference, San Diego, CA, January, 1994)

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Authors
Machczyńska, J., Zimny, J., & Bednarek, P. T.
Affiliations
Plant Breeding and Acclimatization Institute - National Research Institute (PL)
Corresponding author
Bednarek, Piotr Tomasz
Venue
Plant Molecular Biology
Published
2015-09-03
Volume
89
Issue
3
Pages
279-292
ISSN
  • 0167-4412 (ISSN-L)
  • 1573-5028
OpenAlex
W1787947051
Cited by
59
References
79
Citations by year
2025: 2 · 2024: 3 · 2023: 3 · 2022: 12 · 2021: 10 · 2020: 7 · 2019: 10 · 2018: 5 · 2017: 6 · 2016: 1
FWCI
1.74
Type
journal article
Language
en
Open access
Locations
  • Plant Molecular Biology (publishedVersion, cc-by)
  • Europe PMC (PubMed Central) (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Plant tissue culture and regeneration
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Epigenetics and DNA Methylation
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Genetic and Mutation Studies
Keywords
triticale, epigenetic variation, DNA methylation, androgenesis, somatic embryogenesis, plant regeneration, demethylation, de novo methylation, genotype-dependent variation
MeSH terms
Triticale, Genotype, Plant Proteins, Cluster Analysis, Gene Expression Regulation, Plant, Cloning, Organism, Nucleic Acid Amplification Techniques, Epigenesis, Genetic, Tissue Culture Techniques

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