Tissue Culture-Induced Genetic and Epigenetic Variation in Triticale (× Triticosecale Spp. Wittmack Ex A. Camus 1927) Regenerants
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Plant regeneration via in vitro culture can induce genetic and epigenetic variation; however, the extent of such changes in triticale is not yet understood. In the present study, metAFLP, a variation of methylation-sensitive amplified fragment length polymorphism analysis, was used to investigate tissue culture-induced variation in triticale regenerants derived from four distinct genotypes using androgenesis and somatic embryogenesis. The metAFLP technique enabled identification of both sequence and DNA methylation pattern changes in a single experiment. Moreover, it was possible to quantify subtle effects such as sequence variation, demethylation, and de novo methylation, which affected 19, 5.5, 4.5% of sites, respectively. Comparison of variation in different genotypes and with different in vitro regeneration approaches demonstrated that both the culture technique and genetic background of donor plants affected tissue culture-induced variation. The results showed that the metAFLP approach could be used for quantification of tissue culture-induced variation and provided direct evidence that in vitro plant regeneration could cause genetic and epigenetic variation.
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In X Triticosecale spp., 51% of tissue culture-induced variation was observed.
“51% of tissue culture-induced variation”
From Somaclonal Variation and Clonal Fidelity in Commercial Micropropagation: Challenges and Perspectives (2025) · cites this paper as [156]
Results > MetAFLP results, Table 2 (page not provided), reports mean total tissue culture-induced variation of 29.12% across triticale genotypes and regeneration approaches. Its highest listed value is 36.55%, not 51%. (LLM, conf=0.88)
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- Authors
- Machczyńska, J., Zimny, J., & Bednarek, P. T.
- Affiliations
- Plant Breeding and Acclimatization Institute - National Research Institute (PL)
- Corresponding author
- Bednarek, Piotr Tomasz
- Venue
- Plant Molecular Biology
- Published
- 2015-09-03
- Volume
- 89
- Issue
- 3
- Pages
- 279-292
- ISSN
- 0167-4412 (ISSN-L)
- 1573-5028
- PMC
- PMC4579263
- OpenAlex
- W1787947051
- Cited by
- 59
- References
- 79
- Citations by year
- 2025: 2 · 2024: 3 · 2023: 3 · 2022: 12 · 2021: 10 · 2020: 7 · 2019: 10 · 2018: 5 · 2017: 6 · 2016: 1
- FWCI
- 1.74
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Locations
- Plant Molecular Biology (publishedVersion, cc-by)
- Europe PMC (PubMed Central) (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Plant tissue culture and regeneration
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Epigenetics and DNA Methylation
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Genetic and Mutation Studies
- Keywords
- triticale, epigenetic variation, DNA methylation, androgenesis, somatic embryogenesis, plant regeneration, demethylation, de novo methylation, genotype-dependent variation
- MeSH terms
- Triticale, Genotype, Plant Proteins, Cluster Analysis, Gene Expression Regulation, Plant, Cloning, Organism, Nucleic Acid Amplification Techniques, Epigenesis, Genetic, Tissue Culture Techniques
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