Medium Composition Affects the Tissue Culture-Induced Variation in Triticale Regenerants
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These interrelated factors dictate the frequency and type of somaclonal variation, emphasizing the need to optimize genotype selection, explant sourcing, culture media, environmental controls, and subculture protocols to minimize variability.
“These interrelated factors dictate the frequency and type of somaclonal variation, emphasizing the need to carefully optimize genotype selection, explant sourcing, culture media, environmental controls, and subculture protocols to minimize variability in micropropagated plant populations [79,80].”
From Somaclonal Variation and Clonal Fidelity in Commercial Micropropagation: Challenges and Perspectives (2025) · cites this paper as [79]
In X Triticosecale spp., 51% tissue culture-induced variation was observed, and variation was influenced by culture medium composition.
“51% tissue culture-induced culture medium composition. variation was observed, and variation was influenced by”
From Somaclonal Variation and Clonal Fidelity in Commercial Micropropagation: Challenges and Perspectives (2025) · cites this paper as [79]
Modifications in culture medium affect both DNA sequence changes and methylation, offering a way to control the level of variation in tissue culture-derived regenerants.
“The findings suggest that modifications in the culture medium affect both DNA sequence changes and methylation, offering a way to control the level of variation in tissue culture-derived regenerants [79].”
From Somaclonal Variation and Clonal Fidelity in Commercial Micropropagation: Challenges and Perspectives (2025) · cites this paper as [79]
Adjusting nutrient composition, fine-tuning PGR ratios, and limiting the number of subcultures reduce somaclonal variation, as excessive subculturing increases the chance of genetic and epigenetic alterations, particularly in callus and cell suspension cultures.
“This involves adjusting the nutrient composition of culture media, fine-tuning the ratios of plant growth regulators (PGRs), and limiting the number of subcultures, as excessive subculturing increases the chance of genetic and epigenetic alterations, particularly in callus and cell suspension cultures [79,106].”
From Somaclonal Variation and Clonal Fidelity in Commercial Micropropagation: Challenges and Perspectives (2025) · cites this paper as [79]
Optimization of culture conditions plays a vital role in minimizing somaclonal variation.
“In addition, optimization of culture conditions plays a vital role in minimizing somaclonal variation.”
From Somaclonal Variation and Clonal Fidelity in Commercial Micropropagation: Challenges and Perspectives (2025) · cites this paper as [79]
In winter triticale, Cu2+ and Ag+ ions and incubation time affected tissue culture-induced variation and genetic homogeneity, with 51% variation including 43% sequence variation, 5% demethylation, and 3% de novo methylation using metAFLP.
“These results showed a 51% variation, including 43% sequence variation, 5% demethylation, and 3% de novo methylation using metAFLP.”
From Somaclonal Variation and Clonal Fidelity in Commercial Micropropagation: Challenges and Perspectives (2025) · cites this paper as [79]
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Optimization of anther culture of awnless triticale
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Dyduch‐Siemińska, M., & Gawroński, J. (2025) · Genes · doi:10.3390/genes16101203 · Cited by 1 · see citing works
Akinina, V. N., Khomyakova, O. V., Djatchouk, T. I., Zhilin, S. V., Barnashova, E. K., Kalashnikova, E. V., & Kulikova, V. P. (2023) · Grain Economy of Russia · doi:10.31367/2079-8725-2023-85-2-50-56
Orłowska, R., Zimny, J., Żebrowski, J., Androsiuk, P., & Bednarek, P. T. (2024) · Figshare · doi:10.6084/m9.figshare.24979536
Self-citationOrłowska, R., Zimny, J., & Bednarek, P. T. (2026) · Plant Cell Tissue and Organ Culture (PCTOC) · doi:10.1007/s11240-026-03544-6
Self-citation
- Authors
- Pachota, K. A., Orłowska, R., & Bednarek, P. T.
- Affiliations
- Plant Breeding and Acclimatization Institute - National Research Institute (PL)
- Corresponding author
- Orłowska, Renata
- Venue
- Plant Cell, Tissue and Organ Culture (PCTOC)
- Published
- 2022-06-03
- Volume
- 151
- Issue
- 1
- Pages
- 35-46
- ISSN
- 0167-6857 (ISSN-L)
- 1573-5044
- OpenAlex
- W4281749450
- Cited by
- 15
- References
- 85
- Citations by year
- 2026: 1 · 2025: 3 · 2024: 4 · 2023: 2 · 2022: 5
- FWCI
- 1.14
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Plant Cell Tissue and Organ Culture (PCTOC) (publishedVersion)
- Funders
- Ministry of Agriculture and Rural Development, Poland (HORhn-801-PB-22/15-18)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Plant tissue culture and regeneration
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Genetic and Mutation Studies
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Epigenetics and DNA Methylation
- Keywords
- triticale, doubled haploids, plant regeneration, copper ions, silver ions, DNA methylation, demethylation, de novo methylation, genetic homogeneity, haploid lines
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