Cited by 15Plant tissue culture and regenerationPlant Genetic and Mutation StudiesEpigenetics and DNA Methylation

Medium Composition Affects the Tissue Culture-Induced Variation in Triticale Regenerants

Pachota, Katarzyna A., Orłowska, Renata, Bednarek, Piotr T.·2022·Plant Cell, Tissue and Organ Culture (PCTOC)

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Crossref · 10.1007/s11240-022-02327-z

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85 of 85 references verified0 citation errors · 0 not checkable

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  • These interrelated factors dictate the frequency and type of somaclonal variation, emphasizing the need to optimize genotype selection, explant sourcing, culture media, environmental controls, and subculture protocols to minimize variability.

    “These interrelated factors dictate the frequency and type of somaclonal variation, emphasizing the need to carefully optimize genotype selection, explant sourcing, culture media, environmental controls, and subculture protocols to minimize variability in micropropagated plant populations [79,80].”
  • In X Triticosecale spp., 51% tissue culture-induced variation was observed, and variation was influenced by culture medium composition.

    “51% tissue culture-induced culture medium composition. variation was observed, and variation was influenced by”
  • Modifications in culture medium affect both DNA sequence changes and methylation, offering a way to control the level of variation in tissue culture-derived regenerants.

    “The findings suggest that modifications in the culture medium affect both DNA sequence changes and methylation, offering a way to control the level of variation in tissue culture-derived regenerants [79].”
  • Adjusting nutrient composition, fine-tuning PGR ratios, and limiting the number of subcultures reduce somaclonal variation, as excessive subculturing increases the chance of genetic and epigenetic alterations, particularly in callus and cell suspension cultures.

    “This involves adjusting the nutrient composition of culture media, fine-tuning the ratios of plant growth regulators (PGRs), and limiting the number of subcultures, as excessive subculturing increases the chance of genetic and epigenetic alterations, particularly in callus and cell suspension cultures [79,106].”
  • Optimization of culture conditions plays a vital role in minimizing somaclonal variation.

    “In addition, optimization of culture conditions plays a vital role in minimizing somaclonal variation.”

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Authors
Pachota, K. A., Orłowska, R., & Bednarek, P. T.
Affiliations
Plant Breeding and Acclimatization Institute - National Research Institute (PL)
Corresponding author
Orłowska, Renata
Venue
Plant Cell, Tissue and Organ Culture (PCTOC)
Published
2022-06-03
Volume
151
Issue
1
Pages
35-46
ISSN
  • 0167-6857 (ISSN-L)
  • 1573-5044
OpenAlex
W4281749450
Cited by
15
References
85
Citations by year
2026: 1 · 2025: 3 · 2024: 4 · 2023: 2 · 2022: 5
FWCI
1.14
Type
journal article
Language
en
Open access
closed
Locations
Plant Cell Tissue and Organ Culture (PCTOC) (publishedVersion)
Funders
Ministry of Agriculture and Rural Development, Poland (HORhn-801-PB-22/15-18)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Plant tissue culture and regeneration
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Genetic and Mutation Studies
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Epigenetics and DNA Methylation
Keywords
triticale, doubled haploids, plant regeneration, copper ions, silver ions, DNA methylation, demethylation, de novo methylation, genetic homogeneity, haploid lines

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