Eosinophils develop in distinct stages and are recruited to peripheral sites by alternatively activated macrophages

Voehringer, David; van Rooijen, N; Locksley, Richard M

doi:10.1189/jlb.1106686

Cited by 173Asthma and respiratory diseasesIL-33, ST2, and ILC PathwaysEosinophilic Disorders and Syndromes

Eosinophils develop in distinct stages and are recruited to peripheral sites by alternatively activated macrophages

Voehringer, David, van Rooijen, N, Locksley, Richard M·2007·Journal of Leukocyte Biology

DOI 10.1189/jlb.1106686

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Abstract

Eosinophils are associated with allergic diseases and helminth infections. Development of these cells and recruitment to peripheral tissues are only partially understood. Distinct stages of eosinophil development in fetal liver, bone marrow, and blood could be identified using IL-4 reporter mice and mAb against FIRE, Siglec-F, and CCR3. Immature eosinophils were present in the fetal liver and could reconstitute the eosinophil compartment in irradiated recipient mice. In adult mice, eosinophil maturation proceeded from CCR3− to CCR3+ cells in the bone marrow and was accompanied with changes in the transcriptional profile. Eosinophils appeared as activated cells in lung, thymus, lymph nodes, and Peyer’s patches but remained in a resting state in bone marrow, blood, and spleen. Mixed bone marrow chimeras revealed that recruitment to lung and peritoneum was dependent on Stat6 expression in noneosinophils. Alternatively activated macrophages contributed substantially to tissue recruitment of eosinophils, providing a novel basis for development of therapeutic approaches to lower tissue eosinophilia.

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Authors: Voehringer, D., van Rooijen, N., & Locksley, R. M.
Affiliations: Howard Hughes Medical Institute (US)
Department of Molecular Cell Biology, Faculty of Medicine, Vrije Universiteit , Amsterdam , the Netherlands
Corresponding author: Locksley, Richard M
ORCID: Voehringer, David
Locksley, Richard M
Venue: Journal of Leukocyte Biology
Publisher: Oxford University Press (OUP)
Published: 2007-03-05
Volume: 81
Issue: 6
Pages: 1434-1444
ISSN: 0741-5400 (ISSN-L)
1938-3673
DOI: 10.1189/jlb.1106686
OpenAlex: W2149589945
Cited by: 173
References: 51
Citations by year: 2026: 12 · 2025: 6 · 2024: 3 · 2023: 2 · 2022: 2 · 2021: 10 · 2020: 9 · 2019: 4 · 2018: 8 · 2017: 8 · 2016: 9 · 2015: 13 · 2014: 10 · 2013: 14 · 2012: 13
FWCI: 7.98
Type: journal article
Language: en
Open access: closed
Licence: academic.oup.com/pages/standard-publication-reuse-rights (vor)
Locations: Journal of Leukocyte Biology (publishedVersion)
PubMed (publishedVersion)
Funders: National Institutes of Health (AI30663)
Howard Hughes Medical Institute
Sandler Asthma Basic Research Center
Emmy Noether Program of the Deutsche Forschungsgemeinschaft (Vo944/2)
Topics: Health Sciences › Medicine › Physiology › Asthma and respiratory diseases
Life Sciences › Immunology and Microbiology › Immunology › IL-33, ST2, and ILC Pathways
Health Sciences › Medicine › Rheumatology › Eosinophilic Disorders and Syndromes
Keywords: eosinophils, eosinophil development, eosinophil recruitment, M2 macrophages, CCR3, STAT6, IL-4, Siglec-F, fire, fetal liver, bone marrow, tissue eosinophilia, eosinophil maturation, mixed bone marrow chimeras, allergic diseases, helminthiasis
MeSH terms: Animals, Bone Marrow, Cell Differentiation, Cell Movement, Eosinophils, Fetus, Liver, Macrophage Activation, Macrophages, Mice, Inbred BALB C, Organ Specificity, Peritoneal Cavity, Interleukin-4, Receptors, Chemokine, STAT6 Transcription Factor, Mice, Receptors, CCR3

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Eosinophils develop in distinct stages and are recruited to peripheral sites by alternatively activated macrophages

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