Lipoprotein lipase activity is required for cardiac lipid droplet production
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The rodent heart accumulates TGs and lipid droplets during fasting. The sources of heart lipids could be either FFAs liberated from adipose tissue or FAs from lipoprotein-associated TGs via the action of lipoprotein lipase (LpL). Because circulating levels of FFAs increase during fasting, it has been assumed that albumin transported FFAs are the source of lipids within heart lipid droplets. We studied mice with three genetic mutations: peroxisomal proliferator-activated receptor α deficiency, cluster of differentiation 36 (CD36) deficiency, and heart-specific LpL deletion. All three genetically altered groups of mice had defective accumulation of lipid droplet TGs. Moreover, hearts from mice treated with poloxamer 407, an inhibitor of lipoprotein TG lipolysis, also failed to accumulate TGs, despite increased uptake of FFAs. TG storage did not impair maximal cardiac function as measured by stress echocardiography. Thus, LpL hydrolysis of circulating lipoproteins is required for the accumulation of lipids in the heart of fasting mice.
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In rodents with heart-specific LPL deficiency, triglyceride-containing lipid droplets are reduced in the heart.
“Indeed, studies using rodents with a heart-specific LPL deficiency have shown that TG-containing LDs are reduced in the heart [85].”
From Lipoprotein Lipase Regulates Microglial Lipid Droplet Accumulation (2021) · cites this paper as [85]
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- Authors
- Trent, C. M., Yu, S., Hu, Y., Skoller, N., Huggins, L. A., Homma, S., & Goldberg, I. J.
- Affiliations
- Columbia University Irving Medical Center (US)
- Corresponding author
- Goldberg, Ira J.
- ORCID
- Goldberg, Ira J.
- Venue
- Journal of Lipid Research
- Publisher
- Elsevier BV
- Published
- 2014-04
- Volume
- 55
- Issue
- 4
- Pages
- 645-658
- ISSN
- 0022-2275 (ISSN-L)
- PMC
- PMC3966699
- OpenAlex
- W2131620360
- Cited by
- 55
- References
- 69
- Citations by year
- 2026: 1 · 2025: 2 · 2024: 2 · 2023: 5 · 2022: 7 · 2021: 2 · 2020: 5 · 2019: 5 · 2018: 4 · 2017: 5 · 2016: 8 · 2015: 5 · 2014: 4
- FWCI
- 5.04
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- Locations
- Journal of Lipid Research (publishedVersion, cc-by)
- Europe PMC (PubMed Central) (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Funders
- National Heart, Lung, and Blood Institute (R01HL045095, R01HL073029, HL73029, HL45095, R37HL045095)
- Topics
- Health Sciences › Medicine › Cardiology and Cardiovascular Medicine › Lipid metabolism and disorders
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Lipid metabolism and biosynthesis
- Health Sciences › Medicine › Cardiology and Cardiovascular Medicine › Cardiovascular Function and Risk Factors
- Keywords
- lipoprotein lipase, triglyceride accumulation, fasting, free fatty acids, PPARα, CD36, Pluronic F127, cardiac lipid metabolism, cardiac function
- MeSH terms
- Perilipin-2, Perilipin-5, Animals, Fasting, Hydrolysis, Lipoprotein Lipase, Lipoproteins, Male, Membrane Proteins, Mice, Inbred C57BL, Myocardium, Proteins, Systole, Triglycerides, Mice, Knockout, PPAR alpha, Lipid Metabolism, Mice, Lipid Droplets
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