Effects of forced uncoupling protein 1 expression in 3T3–L1 cells on mitochondrial function and lipid metabolism.
Open accessAbstract
Obesity-related increase in body fat mass is a risk factor for many diseases, including type 2 diabetes. Controlling adiposity by targeted modulation of adipocyte enzymes could offer an attractive alternative to current dietary approaches. Brown adipose tissue, which is present in rodents but not in adult humans, expresses the mitochondrial uncoupling protein 1 (UCP1) that promotes cellular energy dissipation as heat. Here, we report on the direct metabolic effects of forced UCP1 expression in white adipocytes derived from a murine (3T3-L1) preadipocyte cell line. After stable integration, the ucp1 gene product was continuously expressed during differentiation and reduced the total lipid accumulation by approximately 30% without affecting other adipocyte markers, such as cytosolic glycerol-3-phosphate dehydrogenase activity and leptin production. The expression of UCP1 also decreased glycerol output and increased glucose uptake, lactate output, and the sensitivity of cellular ATP content to nutrient removal. However, oxygen consumption and beta-oxidation were minimally affected. Together, our results suggest that the reduction in intracellular lipid by constitutive expression of UCP1 reflects a downregulation of fat synthesis rather than an upregulation of fatty acid oxidation.
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In cultured adipocytes, ectopic expression of UCP1 impairs fatty acid synthesis.
“Moreover, in cultured adipocytes, ectopic expression of UCP1 impairs fatty acid synthesis (186, 187).”
From The role of mitochondria in the pathogenesis of type 2 diabetes (2010) · cites this paper as [187]
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- Authors
- Si, Y., Palani, S., Jayaraman, A., & Lee, K.
- Affiliations
- Tufts University (US)
- Texas A&M University (US)
- Corresponding author
- Lee, Kyongbum
- ORCID
- Lee, Kyongbum
- Venue
- Journal of Lipid Research
- Publisher
- Elsevier BV
- Published
- 2007-04
- Volume
- 48
- Issue
- 4
- Pages
- 826-836
- ISSN
- 0022-2275 (ISSN-L)
- OpenAlex
- W2008984486
- Cited by
- 50
- References
- 56
- Citations by year
- 2025: 3 · 2024: 1 · 2023: 1 · 2022: 1 · 2021: 1 · 2019: 6 · 2018: 1 · 2017: 6 · 2016: 1 · 2015: 4 · 2014: 4 · 2013: 2 · 2012: 5
- FWCI
- 2.45
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- Locations
- Journal of Lipid Research (publishedVersion, cc-by)
- PubMed (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Funders
- National Institute of Diabetes and Digestive and Kidney Diseases (1 R21 DK-67228-01, R21DK067228)
- Topics
- Health Sciences › Medicine › Physiology › Adipose Tissue and Metabolism
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Lipid metabolism and biosynthesis
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Peroxisome Proliferator-Activated Receptors
- Keywords
- UCP1, 3T3-L1 adipocytes, white adipocytes, lipid accumulation, adipogenesis, mitochondrial function, fatty acid oxidation, fat synthesis, glucose uptake, oxygen consumption, brown adipose tissue, obesity, type 2 diabetes, energy dissipation
- MeSH terms
- Uncoupling Protein 1, Animals, Cell Differentiation, Fatty Acids, Glycerides, Ion Channels, Lipids, Mitochondria, Biomarkers, Down-Regulation, Mitochondrial Proteins, 3T3-L1 Cells, Lipid Metabolism, Mice, Adipocytes, White
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