Investigation of the compatibility of the skin PAMPA model with topical formulation and acceptor media additives using different assay setups
Abstract
The Skin Parallel Artificial Membrane Permeability Assay (PAMPA) is a 96-well plate-based skin model with an artificial membrane containing free fatty acid, cholesterol, and synthetic ceramide analogs to mimic the stratum corneum (SC) barrier. The current study evaluates the compatibility of lipophilic solvents/penetration enhancer, topical emulsions containing different emulsifier systems, and organic acceptor media additives with the artificial membrane of the assay. Additionally, different assay setups (standard setup: donor in bottom plate versus modified setup: donor in top plate) were compared. Methylparaben (MP), ethylparaben (EP), and propylparaben (PP) were used as model permeants and internal standards for proper assay execution. The permeation order of the parabens (MP > EP > PP) remained the same with different lipophilic solvents, and the ranking of lipophilic solvents was comparable under standard and modified conditions (isopropyl myristate, IPM > dimethyl isosorbide, DMI ≥ propylene glycol, PG > diisopropyl adipate, DIPA). Pre-incubation of the Skin PAMPA plates with IPM, DIPA, and DMI, as well as with formulations that contain non-ionic emulsifiers, and acceptor solutions containing DMSO or EtOH (≤ 50%) for 4 h did not increase the percentage of permeated parabens in the main experiment, suggesting that those compounds do not make the artificial membrane more permeable. High-resolution mass spectrometry confirmed that acceptor solutions with ≤ 50% DMSO or EtO…
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Skin PAMPAs are 96-well plate-based systems with an artificial membrane between donor and acceptor compartments, used as high-throughput assays for preliminary screening and permeation- and stability-related insights into topical and transdermal formulations.
“the skin PAMPAs are 96-well plate-based systems where an artificial membrane is interposed between a donor and an acceptor compartment, in a sandwich-like structure. These systems attracted the attention of researchers as high-throughput assays for the preliminary screening and for obtaining permeation- and stability-related insights into topical and transdermal formulations [14,15].”
From Recreating Human Skin In Vitro: Should the Microbiota Be Taken into Account? (2024) · cites this paper as [14]
Skin PAMPAs contain free fatty acid, cholesterol, and synthetic ceramide analogs mimicking the stratum corneum.
“In order to overcome this limitation, lipid-based skin models have been developed, such as the skin PAMPAs (Parallel Artificial Membrane Permeability Assays), which contain free fatty acid, cholesterol, and synthetic ceramide analogs mimicking the stratum corneum [12,14]”
From Recreating Human Skin In Vitro: Should the Microbiota Be Taken into Account? (2024) · cites this paper as [14]
Cites
LC/MS analysis of stratum corneum lipids: ceramide profiling and discovery
van Smeden, J., Hoppel, L., van der Heijden, R., Hankemeier, T., Vreeken, R. J., & Bouwstra, J. A. (2011) · Journal of Lipid Research · doi:10.1194/jlr.m014456 · Cited by 229 · see citing works
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Human skin models: From healthy to disease-mimetic systems; characteristics and applications
Moniz, T., Costa Lima, S. A., & Reis, S. (2020) · British Journal of Pharmacology · doi:10.1111/bph.15184 · Cited by 80 · see citing works
Rahma, A., Lane, M. E., & Sinkó, B. (2023) · International Journal of Pharmaceutics · doi:10.1016/j.ijpharm.2023.122692 · Cited by 12 · see citing works
Recreating Human Skin In Vitro: Should the Microbiota Be Taken into Account?
Galvan, A., Pellicciari, C., & Calderan, L. (2024) · International Journal of Molecular Sciences · doi:10.3390/ijms25021165 · Cited by 11 · see citing works
- Authors
- Köllmer, M., Mossahebi, P., Sacharow, E., Gorissen, S., Gräfe, N., Evers, D.-H., & Herbig, M. E.
- Affiliations
- Almirall (Germany) (DE)
- Corresponding author
- Köllmer, Melanie
- ORCID
- Herbig, Michael E.
- Venue
- AAPS PharmSciTech
- Published
- 2019-01-24
- Volume
- 20
- Issue
- 2
- Pages
- 89-89
- ISSN
- 1530-9932 (ISSN-L)
- OpenAlex
- W2914233881
- Cited by
- 15
- References
- 1
- Citations by year
- 2024: 5 · 2023: 1 · 2021: 5 · 2020: 3 · 2019: 1
- FWCI
- 0.96
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- AAPS PharmSciTech (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Pharmacology, Toxicology and Pharmaceutics › Pharmaceutical Science › Advancements in Transdermal Drug Delivery
- Health Sciences › Medicine › Dermatology › Contact Dermatitis and Allergies
- Life Sciences › Pharmacology, Toxicology and Pharmaceutics › Pharmaceutical Science › Drug Solubulity and Delivery Systems
- Keywords
- skin permeation, topical formulation, penetration enhancers, emulsifiers, parabens, methylparaben, stratum corneum barrier, isopropyl myristate, dimethyl isosorbide, propylene glycol, dimethyl sulfoxide, ethanol, preincubation
- MeSH terms
- Administration, Topical, Drug Compounding, Emulsions, Humans, Membranes, Artificial, Myristates, Parabens, Permeability, Skin, Propylene Glycol
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