The DOI matches its OpenAlex record; 12 of the 12 references it cites were found in a registry or an index.
Cloning and functional expression of a rat brain corticotropin releasing factor (CRF) receptor
Abstract
Corticotropin releasing factor (CRF), a key neuroregulator of the hypothalamic-pituitary-adrenal cortical axis, also displays a broad range of effects on the endocrine, central nervous and immune systems. Having recently characterized the human pituitary CRF receptor by expression cloning of cDNA from a human Cushing's corticotropic adenoma, we report here the structure of the cDNA for a rat brain CRF receptor (rCRF-R) which was cloned by hybridization from a rat brain cDNA library. The sequence of the rCRF-R encodes a 415 amino acid protein comprising seven membrane spanning domains. The rCRF-R is 97% identical at the amino acid level to the human pituitary tumor CRF receptor, differing by only 12 amino acids. When expressed in COSM6 cells, the rCRF-R binds CRF with high affinity (Kd = 1.7 (0.8-3.8)nM). The receptor transduces a CRF stimulated accumulation of intracellular cAMP which is inhibited by the CRF antagonist, alpha helCRF(9-41). These results suggest that the brain expresses a CRF receptor similar to that in the pituitary.
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Cites
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Self-citationExpression cloning of a human corticotropin-releasing-factor receptor.
Chen, R., Lewis, K. A., Perrin, M. H., & Vale, W. W. (1993) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.90.19.8967 · Cited by 927
Self-citationExpression cloning of an activin receptor, a predicted transmembrane serine kinase
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Self-citationCorticotropin-Releasing Factor Receptors in Rat Forebrain: Autoradiographic Identification
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Self-citationProperties and regulation of high-affinity pituitary receptors for corticotropin-releasing factor
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Cited by
Lewis, K., Li, C., Perrin, M. H., Blount, A., Kunitake, K., Donaldson, C., Vaughan, J., Reyes, T. M., Gulyas, J., Fischer, W., et al. (2001) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.121165198 · Cited by 925
Self-citationHolsboer, F. (1999) · Journal of Psychiatric Research · doi:10.1016/s0022-3956(98)90056-5 · Cited by 654
Corticotropin-releasing factor, norepinephrine, and stress
Koob, G. F. (1999) · Biological Psychiatry · doi:10.1016/s0006-3223(99)00164-x · Cited by 629
A role for corticotropin releasing factor and urocortin in behavioral responses to stressors
Koob, G. F., & Heinrichs, S. C. (1999) · Brain Research · doi:10.1016/s0006-8993(99)01991-5 · Cited by 531
Potter, E., Sutton, S., Donaldson, C., Chen, R., Perrin, M., Lewis, K., Sawchenko, P. E., & Vale, W. (1994) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.91.19.8777 · Cited by 514
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Metadata
- Authors
- Perrin, M. H., Donaldson, C. J., Chen, R., Lewis, K. A., & Vale, W.
- Venue
- Endocrinology
- Publisher
- Oxford University Press
- Published
- 1993-12-01
- Volume
- 133
- Issue
- 6
- Pages
- 3058-3061
- ISSN
- 0013-7227 (ISSN-L)
- OpenAlex
- W1991192480
- Cited by
- 437
- References
- 12
- FWCI
- 10.46
- Type
- journal article
- Language
- en
- Open access
- closed
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- Perrin, M. H., Donaldson, C. J., Chen, R., Lewis, K. A., & Vale, W. (1993). Cloning and functional expression of a rat brain corticotropin releasing factor (CRF) receptor. Endocrinology. https://doi.org/10.1210/en.133.6.3058
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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