Cold Tolerance in Hypothyroid Rabbits: Role of Skeletal Muscle Mitochondria and Sarcoplasmic Reticulum Ca2+ ATPase Isoform 1 Heat Production
Abstract
Brown adipose tissue (BAT) is involved in rat and mice thermoregulation, and heat produced by BAT depends on the concerted action of thyroid hormones and catecholamines. Little is known about cold-induced thermogenesis in mammals that have little or no BAT, such as rabbits. In these animals, thermogenesis primarily occurs in skeletal muscle. In this work, we have studied the effect of cold acclimation (4 C for 10 d) in normal and hypothyroid rabbits. It is known that hypothyroid rats die after a few hours of cold exposure. We now show that, different from rats, hypothyroid rabbits sustain their body temperature and survive after 10 d cold exposure. When compared with rabbits kept at room temperature, the muscles of cold-exposed rabbits showed a dark red color characteristic of oxidative muscle fibers. According to this pattern, we observed that in both normal and hypothyroid rabbits, cold exposure promotes an increase in oxygen consumption by skeletal muscle mitochondria. Moreover, in red muscle, cold acclimation induces an increase in the expression and activity of sarcoplasmic reticulum Ca2+ ATPase isoform 1 (SERCA1), one of the muscle enzymes involved in heat production. We conclude that rabbit cold tolerance is probably related to increased muscle oxidative metabolism and heat production by SERCA1 and that these changes are not completely dependent on normal thyroid function.
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Cold exposure induces expression and increases activity of SERCA-1 in skeletal muscle to increase muscle oxidative metabolism and heat production.
“Cold exposure induces expression and increases activity of SERCA-1 in skeletal muscle to increase muscle oxidative metabolism and heat production 117.”
From Cellular bioenergetics as a target for obesity therapy (2010) · cites this paper as [117]
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- Authors
- Arruda, A. P., Ketzer, L. A., Nigro, M., Galina, A., Carvalho, D. P., & de Meis, L.
- Affiliations
- Instituto de Bioquímica Médica (A.P.A., L.A.K., M.N., A.G., L.d.M.), Cidade Universitária, Rio de Janeiro 21941-590, Brazil
- Universidade Federal do Rio de Janeiro (BR)
- Venue
- Endocrinology
- Publisher
- The Endocrine Society
- Published
- 2008-08-14
- Volume
- 149
- Issue
- 12
- Pages
- 6262-6271
- ISSN
- 0013-7227 (ISSN-L)
- 1945-7170
- OpenAlex
- W2054406852
- Cited by
- 55
- References
- 56
- Citations by year
- 2024: 2 · 2023: 2 · 2022: 3 · 2021: 2 · 2020: 4 · 2019: 1 · 2018: 3 · 2017: 5 · 2016: 4 · 2015: 6 · 2014: 6 · 2013: 3 · 2012: 3
- FWCI
- 2.48
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Endocrinology (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Physiology › Adipose Tissue and Metabolism
- Physical Sciences › Environmental Science › Ecology › Physiological and biochemical adaptations
- Health Sciences › Medicine › Critical Care and Intensive Care Medicine › Thermal Regulation in Medicine
- Keywords
- cold tolerance, hypothyroidism, cold acclimation, skeletal muscle thermogenesis, brown adipose tissue, skeletal muscle mitochondria, SERCA1, sarcoplasmic reticulum Ca2+-ATPase, oxidative muscle fibers, oxygen consumption, thyroid hormones, rabbits, thermoregulation
- MeSH terms
- Animals, Body Weight, Cold Temperature, Eating, Hypothyroidism, Male, Mitochondria, Muscle, Oxygen Consumption, Rabbits, Thyroxine, Time Factors, Triiodothyronine, Blotting, Western, Muscle, Skeletal, Thermogenesis, Sarcoplasmic Reticulum Calcium-Transporting ATPases
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