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Cited by 67Sperm and Testicular FunctionHypothalamic control of reproductive hormonesReproductive Biology and Fertility

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Epididymal Fat Is Necessary for Spermatogenesis, but not Testosterone Production or Copulatory Behavior

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Chu, Ye, Huddleston, Gloria G., Clancy, Andrew N., Harris, Ruth B. S., Bartness, Timothy J.·2010·Endocrinology
DOI 10.1210/en.2010-0772PMC PMC2999502
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Abstract

Surgical removal of the epididymal white adipose tissue (EWAT) depot (lipectomy; EWATx) in laboratory rats or mice decreases spermatogenesis, but this phenomenal finding has not been investigated in depth. Specifically, detailed histology, neuroendocrine profiles, copulatory behavior, lipectomy of other WAT depots, rescue by autologous EWAT transplants, or tests whether this EWATx effect is due to disruption of testes innervation occurring during EWATx have not been performed. Therefore, in the first study, we performed EWATx in male Syrian hamsters and attempted to rescue spermatogenesis by transplanting the removed EWAT to the animal’s subcutaneous dorsum, removed comparable or larger amounts of non-gonadal WAT [inguinal WAT (IWAT)] and conducted mating behavior tests. In a second study we conducted detailed testicular histology and assayed serum LH, FSH, and testosterone (T). In a third study, we surgically denervated the testes without removing EWAT and compared testicular histology with that of EWATx or sham surgery. We found that EWATx, but not IWATx, virtually eliminated spermatogenesis producing a marked decrease in size of the seminiferous tubule cellular lining including the Sertoli cells and spermatogonia that could not be rescued by autologous EWAT transplant to the subcutaneous dorsum. EWATx did not change serum LH or T concentrations but approximately doubled serum FSH concentrations. EWATx did not alter copulatory behavior but resulted in aspermatic ejaculate. Selective surgical testicular denervation did not affect spermatogenesis. Collectively, these results suggest the presence of a local, but currently unidentified, growth and/or nutritive factor from EWAT that promotes spermatogenesis.

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Metadata

Authors
Chu, Y., Huddleston, G. G., Clancy, A. N., Harris, R. B. S., & Bartness, T. J.
Affiliations
  • University of Georgia (US)
  • Georgia State University (US)
Corresponding author
Bartness, Timothy J.
ORCID
Venue
Endocrinology
Publisher
The Endocrine Society
Published
2010-09-29
Volume
151
Issue
12
Pages
5669-5679
ISSN
  • 0013-7227 (ISSN-L)
  • 1945-7170
DOI
10.1210/en.2010-0772
PMC
PMC2999502
OpenAlex
W2130411267
Cited by
67
References
2
Citations by year
2026: 1 · 2025: 2 · 2024: 1 · 2023: 3 · 2022: 6 · 2021: 5 · 2020: 10 · 2019: 3 · 2018: 5 · 2017: 8 · 2016: 4 · 2015: 2 · 2014: 4 · 2013: 3 · 2012: 4
FWCI
4.93
Type
journal article
Language
en
Open access
Locations
  • Endocrinology (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • U.S. Department of Agriculture
  • National Institute of Diabetes and Digestive and Kidney Diseases (R01DK053903, R37 DK-36254)
Topics
  • Health Sciences › Medicine › Reproductive Medicine › Sperm and Testicular Function
  • Health Sciences › Medicine › Reproductive Medicine › Hypothalamic control of reproductive hormones
  • Health Sciences › Medicine › Reproductive Medicine › Reproductive Biology and Fertility
Keywords
epididymal adipose tissue, spermatogenesis, lipectomy, inguinal adipose tissue, testosterone, luteinizing hormone, follicle-stimulating hormone, mating behavior, seminiferous tubules, Sertoli cells, spermatogonia, aspermia
MeSH terms
Animals, Female, Cricetinae, Male, Mesocricetus, Sexual Behavior, Animal, Spermatogenesis, Testis, Testosterone, Adipose Tissue, White

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