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Cited by 21Menopause: Health Impacts and TreatmentsEstrogen and related hormone effectsNeuroscience and Neuropharmacology Research

The effects of long-term treatment with estradiol and medroxyprogesterone acetate on tyrosine hydroxylase fibers and neuron number in the medial prefrontal cortex of aged female rats

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Chisholm, Nioka C., Packard, Alexandria R., Koss, Wendy A., Juraska, Janice M.·2012·Endocrinology
DOI 10.1210/en.2012-1412PMC PMC3512004
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Abstract

Menopausal women often initiate hormone treatment to alleviate the symptoms of menopause. Research suggests that these treatments also affect cognition, and studies in young animals indicate that hormone treatment can alter several neuroanatomical measures. However, very little is known about the effects of long-term hormone treatment on the aging female brain. This study investigated the effects of hormone treatment on neuron number and tyrosine hydroxylase (TH) in the rat medial prefrontal cortex (mPFC). Female Long Evans rats were ovariectomized at middle age (12-13 months) and placed in one of four groups: no replacement (NR) (n = 12), 17β-estradiol (E(2)) (n = 12), E(2) and progesterone (n = 7), or E(2) and medroxyprogesterone acetate (MPA) (n = 10). Animals were euthanized at 20 months, and the brains were Nissl stained; a subset was immunostained for TH [NR (n = 5); E(2) (n = 6); E(2) + MPA (n = 4); E(2) + progesterone (n = 6)]. E(2) was administered through the drinking water, and progestagens were administered via pellets inserted at the nape of the neck. Neuron number and TH fiber density were quantified in the mPFC. Hormone treatment did not alter neuron number. Treatment with E(2) and MPA resulted in greater TH densities than NR in layer 1 (P < 0.05). In layers 2/3, animals receiving E(2) had greater TH densities than NR animals (P < 0.01). These results indicate that long-term hormone treatments alter dopaminergic fibers and potentially the functioning of the aging mPFC.

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  • Manipulating ovarian hormone availability has no effect on cell number in the frontal cortex in 12 month old rats, suggesting that the frontal cortex is more sensitive to gonadal steroids earlier in life.

    “Manipulating ovarian hormone availability has no effect on cell number in the frontal cortex in 12 month old rats, suggesting that the frontal cortex is more sensitive to gonadal steroids earlier in life (Chisholm et al., 2012).”

    From Does puberty mark a transition in sensitive periods for plasticity in the associative neocortex? (2017) · cites this paper as Chisholm et al., 2012

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Authors
Chisholm, N. C., Packard, A. R., Koss, W. A., & Juraska, J. M.
Affiliations
University of Illinois Urbana-Champaign (US)
Corresponding author
Juraska, Janice M.
ORCID
Venue
Endocrinology
Publisher
The Endocrine Society
Published
2012-10-01
Volume
153
Issue
10
Pages
4874-4882
ISSN
  • 0013-7227 (ISSN-L)
  • 1945-7170
DOI
10.1210/en.2012-1412
PMC
PMC3512004
OpenAlex
W2098662651
Cited by
21
References
79
Citations by year
2024: 2 · 2020: 2 · 2018: 2 · 2017: 1 · 2016: 5 · 2015: 2 · 2014: 2 · 2013: 5
FWCI
2.31
Type
journal article
Language
en
Open access
Locations
  • Endocrinology (publishedVersion)
  • Europe PMC (PubMed Central) (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
Funders
National Institute on Aging (AG 022499, R01AG022499)
Topics
  • Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Menopause: Health Impacts and Treatments
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Estrogen and related hormone effects
  • Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
Keywords
estradiol, medroxyprogesterone acetate, menopause, hormone replacement therapy, tyrosine hydroxylase, medial prefrontal cortex, neuron number, brain aging, ovariectomy, female rats, progestogens
MeSH terms
Aging, Animals, Body Weight, Cell Count, Estradiol, Female, Medroxyprogesterone, Nerve Fibers, Neurons, Tyrosine 3-Monooxygenase, Uterus, Prefrontal Cortex, Rats, Long-Evans, Rats

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