Comparison of Two Methods for the Bioassay of Oxytocin
Abstract
Two preparations for the bioassay of oxytocin have been compared—the intact mammary gland of the lactating rat and an isolated strip of the gland. The usual threshold doses were of the same order (25 μU/ml in the test solution), and the accuracies of the assays were not significantly different (p >0.05 for the precision indices of 0.079 and 0.087, respectively). As a result of this investigation it was decided that the in vitro preparation is the one of preference, both for its practical advantages and because arginine vasopressin (AVP) is about 50% less potent on this preparation. Two considerations must be borne in mind when estimating theintrinsic oxytocin-like activity of AVP. 1) A constant ratio of mammotonic or milk-ejecting activity to antidiuretic activity cannot be assumed since these ratios vary from preparation to preparation. 2) In one third of the preparations the dose-response curve of AVP is steeper than that of oxytocin so that a definite ratio cannot be obtained; under these circumstances either a midpoint is taken or a new preparation is set up. A procedure has been developed for estimating the individual quantities of oxytocin and AVP present in a mixture. (Endocrinology85: 600, 1969)
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Practical bioassays for oxytocin were less sensitive than antidiuretic assays.
“Antidiuretic assays benefitted from the extreme sensitivity of the urine concentrating mechanisms of the kidney, but practical bioassays for oxytocin were less sensitive.”
From Measuring Oxytocin and Vasopressin: Bioassays, Immunoassays and Random Numbers (2016) · cites this paper as [42]
Bioassays using the nonpregnant rat uterus and mammary gland could generally detect only oxytocin concentrations above 25 μU/ml (42 pg/ml).
“Bioassays using the nonpregnant rat uterus 49 and mammary gland bioassays 42 could generally detect only concentrations above 25 μU/ml (42 pg/ml).”
From Measuring Oxytocin and Vasopressin: Bioassays, Immunoassays and Random Numbers (2016) · cites this paper as [42]
Cites
The Kinetics of the Inhibition of Thyroidal Iodide Accumulation by Thiocyanate: A Reexamination
Scranton, J. R., NlSSEN, W. M., & Halmi, N. S. (1969) · Endocrinology · doi:10.1210/endo-85-3-603 · Cited by 23 · see citing works
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Measuring Oxytocin and Vasopressin: Bioassays, Immunoassays and Random Numbers
Leng, G., & Sabatier, N. (2016) · Journal of Neuroendocrinology · doi:10.1111/jne.12413 · Cited by 237 · see citing works
“Although oxytocin infusions advance labour, and although there is a modest increase in plasma oxytocin concentrations before delivery in most animal studies, many have doubted whether there is any increase in oxytocin secretion before delivery in women (42,53–55).”
BackgroundMethodBisset, G. W. (1976) · doi:10.1007/978-1-349-02718-7_9 · Cited by 27 · see citing works
Beke, E., Gondran, C., Chovelon, B., Peyrin, É., Holzinger, M., & Ravelet, C. (2025) · Biosensors and Bioelectronics · doi:10.1016/j.bios.2025.117306 · Cited by 16 · see citing works
BIOASSAY OF OXYTOCIN ON THE SUPERFUSED MAMMARY GLAND OF THE MOUSE, USING AN AUTOMATIC APPARATUS
ROBINSON, I. C. A. F., & WALKER, J. M. (1974) · British Journal of Pharmacology · doi:10.1111/j.1476-5381.1974.tb09617.x · Cited by 11 · see citing works
A SENSITIVE METHOD FOR THE ASSAY OF OXYTOCIN IN THE CIRCULATING BLOOD
Vane, J. R., & Williams, K. I. H. (1974) · British Journal of Pharmacology · doi:10.1111/j.1476-5381.1974.tb09713.x · Cited by 1 · see citing works
- Authors
- FABIAN, M., FORSLING, M. L., JONES, J. J., & LEE, J.
- Affiliations
- Charing Cross Hospital (GB)
- Venue
- Endocrinology
- Publisher
- The Endocrine Society
- Published
- 1969-09
- Volume
- 85
- Issue
- 3
- Pages
- 600-603
- ISSN
- 0013-7227 (ISSN-L)
- 1945-7170
- OpenAlex
- W1999005658
- Cited by
- 5
- References
- 1
- Citations by year
- 2025: 1 · 2016: 1
- FWCI
- 0.00
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Endocrinology (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Social Sciences › Psychology › Social Psychology › Neuroendocrine regulation and behavior
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuropeptides and Animal Physiology
- Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Growth Hormone and Insulin-like Growth Factors
- Keywords
- vasopressin, mammary gland, lactating rats, antidiuretic activity, dose-response curves, assay precision
- MeSH terms
- Acetylcholine, Analysis of Variance, Animals, Biological Assay, Female, Histamine H1 Antagonists, Lactation, Mammary Glands, Animal, Methods, Milk, Oxytocin, Phenoxybenzamine, Pregnancy, Serotonin Antagonists, Stimulation, Chemical, Sympatholytics, Vasopressins, Rats, In Vitro Techniques
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