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Cited by 11Neuroendocrine regulation and behaviorNeuropeptides and Animal PhysiologyCardiovascular, Neuropeptides, and Oxidative Stress Research

BIOASSAY OF OXYTOCIN ON THE SUPERFUSED MAMMARY GLAND OF THE MOUSE, USING AN AUTOMATIC APPARATUS

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ROBINSON, I.C.A.F., WALKER, J.M.·1974·British Journal of Pharmacology
DOI 10.1111/j.1476-5381.1974.tb09617.xPMC PMC1776646
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Abstract

A sensitive method of assaying oxytocin using the superfused mouse mammary gland is described. The method is shown to be reliable, accurate and precise. Assays are performed automatically and the preparation is stable for at least 18 hours. The preparation is relatively insensitive to bradykinin, histamine, 5‐hydroxytryptamine, angiotensin, prostaglandin F 2α, adrenaline and noradrenaline. The contractions produced by acetylcholine can be abolished by atropine without the sensitivity to oxytocin being affected. Vasopressin has a variable activity on the preparation; its potency can be as high as one‐fifth that of oxytocin. It is concluded that this method compares favourably with other published methods.

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Crossref · 10.1111/j.1476-5381.1974.tb09617.x

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  • Practical bioassays for oxytocin were less sensitive than antidiuretic assays.

    “Antidiuretic assays benefitted from the extreme sensitivity of the urine concentrating mechanisms of the kidney, but practical bioassays for oxytocin were less sensitive.”

    From Measuring Oxytocin and Vasopressin: Bioassays, Immunoassays and Random Numbers (2016) · cites this paper as [50]

  • The most sensitive oxytocin bioassays used mouse mammary strips and could detect 4 μU/ml (7 pg/ml) in 0.5 ml of sample, but even this was not sufficiently sensitive to measure levels in raw plasma.

    “The most sensitive bioassays used mouse mammary strips and could detect 4 μU/ml (7 pg/ml) in 0.5 ml of sample 50, although even this was still not sufficiently sensitive to measure levels in raw plasma.”

    From Measuring Oxytocin and Vasopressin: Bioassays, Immunoassays and Random Numbers (2016) · cites this paper as [50]

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Authors
ROBINSON, I. C. A. F., & WALKER, J. M.
Affiliations
University of Oxford (GB)
ORCID
ROBINSON, I.C.A.F.
Venue
British Journal of Pharmacology
Publisher
Wiley
Published
1974-03
Volume
50
Issue
3
Pages
409-417
ISSN
  • 0007-1188 (ISSN-L)
  • 1476-5381
DOI
10.1111/j.1476-5381.1974.tb09617.x
PMC
PMC1776646
OpenAlex
W1988040847
Cited by
11
References
30
Citations by year
2025: 1 · 2022: 1 · 2016: 1
FWCI
4.47
Type
journal article
Language
en
Open access
Licence
onlinelibrary.wiley.com/termsAndConditions#vor (vor, 14020 d)
Locations
  • British Journal of Pharmacology (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Social Sciences › Psychology › Social Psychology › Neuroendocrine regulation and behavior
  • Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuropeptides and Animal Physiology
  • Health Sciences › Medicine › Surgery › Cardiovascular, Neuropeptides, and Oxidative Stress Research
Keywords
oxytocin, bioassay, mouse mammary gland, superfusion, uterine contraction, vasopressin, bradykinin, acetylcholine, atropine, prostaglandin F2alpha, histamine, epinephrine, norepinephrine
MeSH terms
Acetylcholine, Angiotensin II, Animals, Atropine, Autoanalysis, Biological Assay, Bradykinin, Epinephrine, Female, Histamine, Mammary Glands, Animal, Methods, Norepinephrine, Oxytocin, Perfusion, Prostaglandins, Serotonin, Time Factors, Vasopressins, Mice, In Vitro Techniques

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