Cited by 101Adipose Tissue and MetabolismHormonal Regulation and HypertensionEstrogen and related hormone effects

Aldosterone stimulated differentiation of mouse 3T3-L1 cells into adipocytes

Rondinone, C M, Rodbard, D, Baker, M E·1993·Endocrinology

Abstract

We find that 1-10 nM aldosterone can induce differentiation of 3T3-L1 cells into adipose cells as evaluated by microscopic accumulation of fat droplets and quantitative measurement of triglycerides and of glycerol-3-phosphate dehydrogenase, an enzyme specific for adipocyte differentiation. Moreover, the aldosterone antagonist ZK91587 inhibits aldosterone-but not glucocorticoid-mediated differentiation of 3T3-L1 cells. Steroid binding assays with 3T3-L1 cells indicate the presence of specific binding sites for aldosterone. We conclude that there is an aldosterone receptor-mediated pathway for terminal differentiation of 3T3-L1 cells into adipose cells. Receptors for aldosterone have also been found in a variety of cells that do not function to regulate sodium and potassium transport. The aldosterone receptor may have a role in regulation expression of genes involved in differentiation of these cells.

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Crossref · 10.1210/endo.132.6.8504747

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  • Mineralocorticoid receptors on adipocytes promote inflammatory adipokine expression, leading to lower insulin receptor expression and impaired insulin-induced glucose uptake.

    “MRs on adipocytes promoted the expression of inflammatory adipokines (-), giving rise to lower insulin receptor expression and impaired insulin-induced glucose uptake ().”

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Authors
Rondinone, C. M., Rodbard, D., & Baker, M. E.
Affiliations
National Institutes of Health (US)
Venue
Endocrinology
Published
1993-06
Volume
132
Issue
6
Pages
2421-2426
ISSN
  • 0013-7227 (ISSN-L)
  • 1945-7170
OpenAlex
W2073760548
Cited by
101
References
3
Citations by year
2026: 3 · 2025: 1 · 2024: 4 · 2023: 1 · 2022: 3 · 2021: 3 · 2020: 2 · 2019: 4 · 2018: 5 · 2017: 2 · 2016: 5 · 2015: 5 · 2014: 9 · 2013: 5 · 2012: 6
FWCI
0.00
Type
journal article
Language
en
Open access
closed
Locations
  • Endocrinology (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Health Sciences › Medicine › Physiology › Adipose Tissue and Metabolism
  • Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Hormonal Regulation and Hypertension
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Estrogen and related hormone effects
Keywords
aldosterone, adipogenesis, 3T3-L1 cells, mineralocorticoid receptor, glucocorticoid receptor, steroid binding sites, triglycerides, glycerol-3-phosphate dehydrogenase, gene expression regulation
MeSH terms
Adipose Tissue, Aldosterone, Androstanols, Animals, Binding, Competitive, Cell Differentiation, Glycerolphosphate Dehydrogenase, Hydrocortisone, Receptors, Glucocorticoid, Spironolactone, Triglycerides, 3T3 Cells, Mice

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