Bone Morphogenetic Protein 2 Stimulates Osteoclast Differentiation and Survival Supported by Receptor Activator of Nuclear Factor-κB Ligand
Abstract
Bone is a major storage site for TGFbeta superfamily members, including TGFbeta and bone morphogenetic proteins. It is believed that these cytokines are released from bone during bone resorption. Recent studies have shown that both RANKL and macrophage colony-stimulating factor are two essential factors produced by osteoblasts for inducing osteoclast differentiation. In the present study we examined the effects of bone morphogenetic protein-2 on osteoclast differentiation and survival supported by RANKL and/or macrophage colony-stimulating factor. Mouse bone marrow-derived macrophages differentiated into osteoclasts in the presence of RANKL and macrophage colony-stimulating factor. TGFbeta superfamily members such as bone morphogenetic protein-2, TGFbeta, and activin A markedly enhanced osteoclast differentiation induced by RANKL and macrophage colony-stimulating factor, although each cytokine alone failed to induce osteoclast differentiation in the absence of RANKL. Addition of a soluble form of bone morphogenetic protein receptor type IA to the culture markedly inhibited not only osteoclast formation induced by RANKL and bone morphogenetic protein-2, but also the basal osteoclast formation supported by RANKL alone. Either RANKL or macrophage colony-stimulating factor stimulated the survival of purified osteoclasts. Bone morphogenetic protein-2 enhanced the survival of purified osteoclasts supported by RANKL, but not by macrophage colony-stimulating factor. Both bone marrow…
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Bone morphogenetic protein-1 in conjunction with RANKL increases the differentiation and survival of osteoclasts.
“Bone morphogenetic protein-1 in conjunction with RANKL has been shown to increase the differentiation and survival of osteoclasts [36].”
From Osteoclasts and Microgravity (2020) · cites this paper as [36]
Cites
Yoshida, H., Hayashi, S.-I., Kunisada, T., Ogawa, M., Nishikawa, S., Okamura, H., Sudo, T., Shultz, L. D., & Nishikawa, S.-I. (1990) · Nature · doi:10.1038/345442a0 · Cited by 1,789 · see citing works
Yasuda, H., Shima, N., Nakagawa, N., Mochizuki, S.-I., Yano, K., Fujise, N., Sato, Y., Goto, M., Yamaguchi, K., Kuriyama, M., et al. (1998) · Endocrinology · doi:10.1210/endo.139.3.5837 · Cited by 1,201 · see citing works
Self-citationUdagawa, N., Horwood, N. J., Elliott, J., Mackay, A., Owens, J., Okamura, H., Kurimoto, M., Chambers, T. J., Martin, T. J., & Gillespie, M. T. (1997) · The Journal of Experimental Medicine · doi:10.1084/jem.185.6.1005 · Cited by 378 · see citing works
Self-citationItoh, K., Udagawa, N., Matsuzaki, K., Takami, M., Amano, H., Shinki, T., Ueno, Y., Takahashi, N., & Suda, T. (2000) · Journal of Bone and Mineral Research · doi:10.1359/jbmr.2000.15.9.1766 · Cited by 90 · see citing works
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A Review of the Clinical Side Effects of Bone Morphogenetic Protein-2
James, A. W., LaChaud, G., Shen, J., Asatrian, G., Nguyen, V., Zhang, X., Ting, K., & Soo, C. (2016) · Tissue Engineering Part B: Reviews · doi:10.1089/ten.teb.2015.0357 · Cited by 1,131 · see citing works
Transforming Growth Factor-β1 to the Bone
Janssens, K., ten Dijke, P., Janssens, S., & Van Hul, W. (2005) · Endocrine Reviews · doi:10.1210/er.2004-0001 · Cited by 710 · see citing works
Pederson, L., Ruan, M., Westendorf, J. J., Khosla, S., & Oursler, M. J. (2008) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0805133106 · Cited by 556 · see citing works
Zara, J. N., Siu, R. K., Zhang, X., Shen, J., Ngo, R., Lee, M., Li, W., Chiang, M., Chung, J., Kwak, J., et al. (2011) · Tissue Engineering Part A · doi:10.1089/ten.tea.2010.0555 · Cited by 560 · see citing works
Safety Profile for the Clinical Use of Bone Morphogenetic Proteins in the Spine
Poynton, A. R., & Lane, J. M. (2002) · Spine · doi:10.1097/00007632-200208151-00010 · Cited by 403 · see citing works
Calcitonin Receptor Plays a Physiological Role to Protect Against Hypercalcemia in Mice
Davey, R. A., Turner, A. G., McManus, J. F., Chiu, W. M., Tjahyono, F., Moore, A. J., Atkins, G. J., Anderson, P. H., Ma, C., Glatt, V., et al. (2008) · Journal of Bone and Mineral Research · doi:10.1359/jbmr.080310 · Cited by 87 · see citing works
Smith, J. K. (2020) · Life · doi:10.3390/life10090207 · Cited by 52 · see citing works
- Authors
- Itoh, K., Udagawa, N., Katagiri, T., Iemura, S., Ueno, N., Yasuda, H., Higashio, K., Quinn, J. M. W., Gillespie, M. T., Martin, T. J., Suda, T., & Takahashi, N.
- Affiliations
- SHOWA Medical University (JP)
- National Institute for Basic Biology (JP)
- Institute of Medical Science, The University of Tokyo (JP)
- Megmilk Snow Brand (Japan) (JP)
- St Vincents Institute of Medical Research (AU)
- ORCID
- Venue
- Endocrinology
- Publisher
- The Endocrine Society
- Published
- 2001-08
- Volume
- 142
- Issue
- 8
- Pages
- 3656-3662
- ISSN
- 0013-7227 (ISSN-L)
- 1945-7170
- OpenAlex
- W2120642348
- Cited by
- 250
- References
- 4
- Citations by year
- 2026: 4 · 2025: 1 · 2024: 4 · 2023: 3 · 2022: 7 · 2021: 12 · 2020: 13 · 2019: 7 · 2018: 9 · 2017: 6 · 2016: 14 · 2015: 13 · 2014: 17 · 2013: 14 · 2012: 13
- FWCI
- 3.61
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Endocrinology (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Bone Metabolism and Diseases
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › TGF-β signaling in diseases
- Health Sciences › Medicine › Rheumatology › Rheumatoid Arthritis Research and Therapies
- Keywords
- BMP-2, osteoclastogenesis, osteoclast survival, RANKL, macrophage colony-stimulating factor, TGF-beta superfamily, TGF-beta, activin A, NF-κB activation, bone resorption, bone marrow-derived macrophages
- MeSH terms
- Animals, Bone Marrow Cells, Carrier Proteins, Cell Differentiation, Cell Survival, Cells, Cultured, Humans, Macrophages, Male, Membrane Glycoproteins, Osteoclasts, Macrophage Colony-Stimulating Factor, Transforming Growth Factor beta, Receptors, Tumor Necrosis Factor, Bone Morphogenetic Proteins, Mice, RANK Ligand, Receptor Activator of Nuclear Factor-kappa B, Bone Morphogenetic Protein 2
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