Cited by 1,201Bone Metabolism and DiseasesBone health and osteoporosis researchBone and Dental Protein Studies

Identity of Osteoclastogenesis Inhibitory Factor (OCIF) and Osteoprotegerin (OPG): A Mechanism by which OPG/OCIF Inhibits Osteoclastogenesis in Vitro1

Yasuda, Hisataka, Shima, Nobuyuki, Nakagawa, Nobuaki, Mochizuki, Shin-Ichi, Yano, Kazuki, Fujise, Nobuaki, Sato, Yasushi, Goto, Masaaki +7 more·1998·Endocrinology

Abstract

The morphogenesis and remodeling of bone depends on the integrated activity of osteoblasts that form bone and osteoclasts that resorb bone. We previously reported the isolation of a new cytokine termed osteoclastogenesis inhibitory factor, OCIF, which specifically inhibits osteoclast development. Here we report the cloning of a complementary DNA of human OCIF. OCIF is identical to osteoprotegerin (OPG), a soluble member of the tumor-necrosis factor receptor family that inhibits osteoclastogenesis. Recombinant human OPG/OCIF specifically acts on bone tissues and increases bone mineral density and bone volume associated with a decrease of active osteoclast number in normal rats. Osteoblasts or bone marrow-derived stromal cells support osteoclastogenesis through cell-to-cell interactions. A single class of high affinity binding sites for OPG/OCIF appears on a mouse stromal cell line, ST2, in response to 1,25-dihydroxyvitamin D3. An anti-OPG/OCIF antibody that blocks the binding abolishes the biological activity of OPG/OCIF. When the sites are blocked with OPG/OCIF, ST2 cells fail to support osteoclastogenesis. These results suggest that the sites are involved in cell-to-cell signaling between stromal cells and osteoclast progenitors and that OPG/OCIF inhibits osteoclastogenesis by interrupting the signaling through the sites.

Integrity signals

Registry

Crossref · 10.1210/endo.139.3.5837

Cites

35 of 35 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Claims the paper makes

This paper’s full text isn’t openly available, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Cites

Flagged first, then by citations

Cited by

Sorted by citations

Authors
Yasuda, H., Shima, N., Nakagawa, N., Mochizuki, S.-I., Yano, K., Fujise, N., Sato, Y., Goto, M., Yamaguchi, K., Kuriyama, M., Kanno, T., Murakami, A., Tsuda, E., Morinaga, T., & Higashio, K.
Affiliations
Megmilk Snow Brand (Japan) (JP)
Corresponding author
Higashio, Kanji
Venue
Endocrinology
Published
1998-03
Volume
139
Issue
3
Pages
1329-1337
ISSN
  • 0013-7227 (ISSN-L)
  • 1945-7170
OpenAlex
W2167886526
Cited by
1,201
References
33
Citations by year
2026: 7 · 2025: 13 · 2024: 21 · 2023: 15 · 2022: 21 · 2021: 21 · 2020: 29 · 2019: 36 · 2018: 22 · 2017: 38 · 2016: 22 · 2015: 28 · 2014: 36 · 2013: 54 · 2012: 35
FWCI
24.00
Type
journal article
Language
en
Open access
closed
Locations
  • Endocrinology (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Bone Metabolism and Diseases
  • Health Sciences › Medicine › Orthopedics and Sports Medicine › Bone health and osteoporosis research
  • Health Sciences › Medicine › Rheumatology › Bone and Dental Protein Studies
Keywords
osteoprotegerin, osteoclastogenesis inhibition, TNF receptor superfamily, bone remodeling, bone mineral density, stromal cells, osteoblasts, cell-to-cell signaling, 1,25-dihydroxyvitamin D3, bone resorption, gene cloning
MeSH terms
Amino Acid Sequence, Animals, Cells, Cultured, Cloning, Molecular, Glycoproteins, Humans, Male, Molecular Sequence Data, Osteoclasts, Recombinant Proteins, Bone Density, Bone Remodeling, Rats, Sprague-Dawley, Receptors, Tumor Necrosis Factor, Receptors, Cytoplasmic and Nuclear, Mice, Rats, Osteoprotegerin

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review