The DOI matches its Crossref record.
Assay Variation Confounds the Diagnosis of Hypovitaminosis D: A Call for Standardization
Abstract
Endemic hypovitaminosis D contributes to osteoporosis development. However, variation in 25-hydroxyvitamin D (25OHD) measurement is reported and confounds the diagnosis of vitamin D insufficiency/deficiency. This report emphasizes the marked variability observed in serum 25OHD measurements between laboratories.Initially, postmenopausal women had serum 25OHD determinations: 42 in laboratory A, 20 in laboratory B. Their mean (sem) serum 25OHD concentrations were 46 (2.1) and 21 (2.3) ng/ml in laboratories A and B, respectively. Furthermore, there was little overlap in serum 25OHD among these clinically similar individuals. Specifically, 17% of those measured in laboratory A but 90% in laboratory B were below an arbitrary threshold value of 32 ng/ml.Subsequently, serum was obtained from 10 healthy adults. Two aliquots from each individual, one of which was spiked with 20 ng/ml 25OHD, were sent to six laboratories. Substantial variability was noted between these six laboratories. The mean serum 25OHD concentration ranged from 17.1-35.6 ng/ml. Similarly, the mean increase produced by spiking with 20 ng/ml ranged from 7.7-18.0 ng/ml.In conclusion, 25OHD assays yield markedly differing results; whether an individual is found to have low or normal vitamin D status is a function of the laboratory used. If the medical community is to make progress in correcting widespread hypovitaminosis D, 25OHD measurement must be standardized.
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Cited by
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Holick, M. F., & Chen, T. C. (2008) · The American Journal of Clinical Nutrition · doi:10.1093/ajcn/87.4.1080s · Cited by 2,689
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Hossein-nezhad, A., & Holick, M. F. (2013) · Mayo Clinic Proceedings · doi:10.1016/j.mayocp.2013.05.011 · Cited by 1,298
Holick, M. F., Siris, E. S., Binkley, N., Beard, M. K., Khan, A., Katzer, J. T., Petruschke, R. A., Chen, E., & de Papp, A. E. (2005) · The Journal of Clinical Endocrinology & Metabolism · doi:10.1210/jc.2004-2364 · Cited by 886
Self-citationPludowski, P., Holick, M. F., Pilz, S., Wagner, C. L., Hollis, B. W., Grant, W. B., Shoenfeld, Y., Lerchbaum, E., Llewellyn, D. J., Kienreich, K., et al. (2013) · Autoimmunity Reviews · doi:10.1016/j.autrev.2013.02.004 · Cited by 836
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Reid, I. R., Bolland, M. J., & Grey, A. (2014) · The Lancet · doi:10.1016/s0140-6736(13)61647-5 · Cited by 678
Standardizing vitamin D assays: The way forward
Binkley, N., Sempos, C. T., & (VDSP), F. T. V. D. S. P. (2014) · Journal of Bone and Mineral Research · doi:10.1002/jbmr.2252 · Cited by 204
Self-citationfor the CORTICUS Study Group, Briegel, J., Sprung, C. L., Annane, D., Singer, M., Keh, D., Moreno, R., Möhnle, P., Weiss, Y., Avidan, A., et al. (2009) · Intensive Care Medicine · doi:10.1007/s00134-009-1627-9 · Cited by 93
Vitamin D Deficiency: Implications in the Rehabilitation Setting
Heath, K. M., & Elovic, E. P. (2006) · American Journal of Physical Medicine & Rehabilitation · doi:10.1097/01.phm.0000242622.23195.61 · Cited by 85
Assessment of ACTH assay variability: a multicenter study
Pecori Giraldi, F., Saccani, A., Cavagnini, F., & _, _. (2011) · European Journal of Endocrinology · doi:10.1530/eje-10-0962 · Cited by 80
Vitamin D deficiency in pregnancy – still a public health issue
McAree, T., Jacobs, B., Manickavasagar, T., Velagala, S., Brennan, L., Bassett, P., Rainbow, S., & Blair, M. (2013) · Maternal & Child Nutrition · doi:10.1111/mcn.12014 · Cited by 79
Diehl, J. J. E., Baines, F. M., Heijboer, A. C., van Leeuwen, J. P., Kik, M., Hendriks, W. H., & Oonincx, D. G. A. B. (2018) · Journal of Animal Physiology and Animal Nutrition · doi:10.1111/jpn.12728 · Cited by 15
Metadata
- Authors
- Binkley, N., Krueger, D., Cowgill, C. S., Plum, L., Lake, E., Hansen, K. E., DeLuca, H. F., & Drezner, M. K.
- Venue
- The Journal of Clinical Endocrinology & Metabolism
- Publisher
- The Endocrine Society
- Published
- 2004-07
- Volume
- 89
- Issue
- 7
- Pages
- 3152-3157
- ISSN
- 0021-972X (ISSN-L)
- 1945-7197
- OpenAlex
- W2042517103
- Cited by
- 577
- References
- 0
- FWCI
- 26.42
- Type
- journal article
- Language
- en
- Open access
- closed
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