Estrogen Receptor β, But Not Estrogen Receptor α, Is Present in the Vascular Endothelium of the Human and Nonhuman Primate Endometrium1
Abstract
Estrogen action is dependent upon the presence of specific ligand-activated receptors in target tissues. The aim of the present experiments was to compare the spatial and temporal pattern of expression of estrogen receptor beta (ERbeta) with that of ERalpha in full thickness endometrial samples (from the superficial to the basal zone) obtained from both women and rhesus macaques. Immunohistochemical localization with specific antibodies revealed that ERalpha and ERbeta were both expressed in nuclei of the glands and stroma. Consistent with previous studies, expression of ERalpha declined in the glands and stroma of the functionalis during the secretory phase. The luminal epithelium also displayed positive immunoreactivity for ERbeta. Expression of ERbeta declined in glandular cell nuclei, but not stroma, within the functionalis during the late secretory phase. Levels of expression of ERalpha and ERbeta in all cellular compartments remained unchanged in the basalis. Both receptor subtypes were detected on Western blots using proteins extracted from uterine samples obtained throughout the menstrual cycle. There was a striking contrast between the pattern of expression of ERalpha and ERbeta in the vascular endothelium and the perivascular cells surrounding endometrial blood vessels; only ERbeta was present in the endothelial cell population, although both forms of ER were expressed in perivascular cells. We conclude that estrogen action(s) within the vascular endothelium in the…
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In the human endometrium, ERalpha and ERbeta exhibit cell-specific patterns of expression during the menstrual cycle.
“In the human endometrium, ERalpha and ERbeta exhibit cell-specific patterns of expression during the menstrual cycle [29].”
From Endometrial Intracrinology: Oestrogens, Androgens and Endometrial Disorders (2018) · cites this paper as [29]
Detailed immunohistochemical studies using fixed full-thickness endometrial tissues with validated antibodies have identified nuclear staining for AR, ERalpha and ERbeta in the human endometrium during the normal menstrual cycle.
“Detailed immunohistochemical studies using fixed full-thickness endometrial tissues in combination with validated antibodies [27] have identified nuclear staining for AR, ERalpha and ERbeta in the human endometrium during the normal menstrual cycle [16,18,28,29].”
From Endometrial Intracrinology: Oestrogens, Androgens and Endometrial Disorders (2018) · cites this paper as [29]
ERbeta protein is present in endothelial cells and multiple populations of immune cells that are ERalpha-negative.
“Immunolocalisation of ERbeta protein suggests it does not mirror the dynamic change in expression in stromal or epithelial cells seen with ERalpha and that the protein is present in endothelial cells and multiple populations of immune cells that are ERalpha-negative [17,18,29].”
From Endometrial Intracrinology: Oestrogens, Androgens and Endometrial Disorders (2018) · cites this paper as [29]
ERalpha is present in the epithelial cells lining the glands and lumen during the proliferative phase and is downregulated during the secretory phase.
“Notably, ERalpha is present in the epithelial cells lining the glands and lumen during the proliferative phase, at a time when circulating oestrogens are rising rapidly due to growth of antral follicles containing granulosa cells expressing aromatase [31], but is downregulated during the secretory phase [29].”
From Endometrial Intracrinology: Oestrogens, Androgens and Endometrial Disorders (2018) · cites this paper as [29]
Cites
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- Authors
- Critchley, H. O. D., Brenner, R. M., Henderson, T. A., Williams, K., Nayak, N. R., Slayden, O. D., Millar, M. R., & Saunders, P. T. K.
- Affiliations
- University of Edinburgh (GB)
- Oregon National Primate Research Center (US)
- Medical Research Council (GB)
- ORCID
- Venue
- The Journal of Clinical Endocrinology & Metabolism
- Publisher
- The Endocrine Society
- Published
- 2001-03-01
- Volume
- 86
- Issue
- 3
- Pages
- 1370-1378
- ISSN
- 0021-972X (ISSN-L)
- 1945-7197
- OpenAlex
- W2116041308
- Cited by
- 210
- References
- 2
- Citations by year
- 2024: 1 · 2023: 2 · 2022: 9 · 2021: 3 · 2020: 6 · 2019: 6 · 2018: 6 · 2017: 5 · 2016: 2 · 2015: 6 · 2014: 10 · 2013: 10 · 2012: 8
- FWCI
- 9.93
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- The Journal of Clinical Endocrinology & Metabolism (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Estrogen and related hormone effects
- Physical Sciences › Environmental Science › Health, Toxicology and Mutagenesis › Effects and risks of endocrine disrupting chemicals
- Health Sciences › Medicine › Obstetrics and Gynecology › Endometrial and Cervical Cancer Treatments
- Keywords
- estrogen receptor beta, estrogen receptor alpha, endometrium, vascular endothelium, menstrual cycle, luteal phase, rhesus monkeys, perivascular cells, selective estrogen receptor modulators, estrogen signaling
- MeSH terms
- Adult, Animals, Antibodies, Monoclonal, Antibody Specificity, Cell Nucleus, Endometrium, Endothelium, Vascular, Female, Humans, Immunohistochemistry, Macaca mulatta, Menstrual Cycle, Middle Aged, Receptors, Estrogen, Recombinant Proteins, Species Specificity, Blotting, Western, Estrogen Receptor alpha, Estrogen Receptor beta
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