Student or educator? Sign up with your school email and get 6 months of Pro free.
CoChat
Sign Up
Cited by 421

The DOI matches its Crossref record; 5 of the 5 references it cites were found in a registry or an index.

NFATc1 Induces Osteoclast Fusion Via Up-Regulation of Atp6v0d2 and the Dendritic Cell-Specific Transmembrane Protein (DC-STAMP)

Kim, Kabsun, Lee, Seoung-Hoon, Ha Kim, Jung, Choi, Yongwon, Kim, Nacksung·2008·Molecular Endocrinology
DOI 10.1210/me.2007-0237
Open this paper in CoChat — free account

Abstract

NFATc1 has been characterized as a master regulator of nuclear factor κB ligand-induced osteoclast differentiation. Herein, we demonstrate a novel role for NFATc1 as a positive regulator of nuclear factor κB ligand-mediated osteoclast fusion as well as other fusion-inducing factors such as TNF-α. Exogenous overexpression of a constitutively active form of NFATc1 in bone marrow-derived monocyte/macrophage cells (BMMs) induces formation of multinucleated osteoclasts as well as the expression of fusion-mediating molecules such as the d2 isoform of vacuolar ATPase Vo domain (Atp6v0d2) and the dendritic cell-specific transmembrane protein (DC-STAMP). Moreover, inactivation of NFATc1 by cyclosporin A treatment attenuates expression of Atp6v0d2 and DC-STAMP and subsequent fusion process of osteoclasts. We show that NFATc1 binds to the promoter regions of Atp6v0d2 and DC-STAMP in osteoclasts and directly induces their expression. Furthermore, overexpression of Atp6v0d2 and DC-STAMP rescues cell-cell fusion of preosteoclasts despite reduced NFATc1 activity. Our data indicate for the first time that the NFATc1/Atp6v0d2 and DC-STAMP signaling axis plays a key role in the osteoclast multinucleation process, which is essential for efficient bone resorption.

Overview

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Integrity signals

Registry

Crossref · 10.1210/me.2007-0237

Cites

5 of 5 references verified0 citation errors · 0 not checkable

Statistics

Verification

Claims the paper makes

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Self-check

Percentages, sums and reported differences re-computed from the paper’s own numbers.

The self-check needs the paper’s full text.

Citations

Cites

Show all 5 references

Research works printed but not linked: 32

  1. Suda, 1999, Modulation of osteoclast differentiation and function by the new members of the tumor necrosis factor receptor and ligand families., Endocr Rev, 20, 345, 10.1210/edrv.20.3.0367 · DOI
  2. Lacey, 1998, Osteoprotegerin ligand is a cytokine that regulates osteoclast differentiation and activation., Cell, 93, 165, 10.1016/S0092-8674(00)81569-X · DOI · Paper page
  3. Wong, 1997, TRANCE (tumor necrosis factor [TNF]-related activation-induced cytokine), a new TNF family member predominantly expressed in T cells, is a dendritic cell-specific survival factor., J Exp Med, 186, 2075, 10.1084/jem.186.12.2075 · DOI · Paper page
  4. Yasuda, 1998, Osteoclast differentiation factor is a ligand for osteoprotegerin/osteoclastogenesis-inhibitory factor and is identical to TRANCE/RANKL., Proc Natl Acad Sci USA, 95, 3597, 10.1073/pnas.95.7.3597 · DOI
  5. Anderson, 1997, A homologue of the TNF receptor and its ligand enhance T-cell growth and dendritic-cell function., Nature, 390, 175, 10.1038/36593 · DOI · Paper page
  6. Teitelbaum, 2000, Bone resorption by osteoclasts., Science, 289, 1504, 10.1126/science.289.5484.1504 · DOI · Paper page
  7. Teitelbaum, 2003, Genetic regulation of osteoclast development and function., Nat Rev Genet, 4, 638, 10.1038/nrg1122 · DOI
  8. Walsh, 2006, Osteoimmunology: interplay between the immune system and bone metabolism., Annu Rev Immunol, 24, 33, 10.1146/annurev.immunol.24.021605.090646 · DOI · Paper page
  9. Abe, 1999, Meltrin-α, a fusion protein involved in multinucleated giant cell and osteoclast formation., Calcif Tissue Int, 64, 508, 10.1007/s002239900641 · DOI
  10. Vries, 2005, Effect of CD44 deficiency on in vitro and in vivo osteoclast formation., J Cell Biochem, 94, 954, 10.1002/jcb.20326 · DOI
  11. Mbalaviele, 1995, The role of cadherin in the generation of multinucleated osteoclasts from mononuclear precursors in murine marrow., J Clin Invest, 95, 2757, 10.1172/JCI117979 · DOI
  12. Saginario, 1998, MFR, a putative receptor mediating the fusion of macrophages., Mol Cell Biol, 18, 6213, 10.1128/MCB.18.11.6213 · DOI
  13. Takeda, 2003, Tetraspanins CD9 and CD81 function to prevent the fusion of mononuclear phagocytes., J Cell Biol, 161, 945, 10.1083/jcb.200212031 · DOI
  14. Lee, 2006, v-ATPase V(0) subunit d2-deficient mice exhibit impaired osteoclast fusion and increased bone formation., Nat Med, 12, 1403, 10.1038/nm1514 · DOI
  15. Hirotani, 2004, The calcineurin/nuclear factor of activated T cells signaling pathway regulates osteoclastogenesis in RAW264.7 cells., J Biol Chem, 279, 13984, 10.1074/jbc.M213067200 · DOI
  16. Komarova, 2005, Convergent signaling by acidosis and receptor activator of NF-κB ligand (RANKL) on the calcium/calcineurin/NFAT pathway in osteoclasts., Proc Natl Acad Sci USA, 102, 2643, 10.1073/pnas.0406874102 · DOI
  17. Crotti, 2006, NFATc1 regulation of the human β3 integrin promoter in osteoclast differentiation., Gene, 372, 92, 10.1016/j.gene.2005.12.012 · DOI
  18. Ikeda, 2006, Activation of NFAT signal in vivo leads to osteopenia associated with increased osteoclastogenesis and bone-resorbing activity., J Immunol, 177, 2384, 10.4049/jimmunol.177.4.2384 · DOI
  19. Kim, 2005, Nuclear factor of activated T cells c1 induces osteoclast-associated receptor gene expression during tumor necrosis factor-related activation-induced cytokine-mediated osteoclastogenesis., J Biol Chem, 280, 35209, 10.1074/jbc.M505815200 · DOI
  20. Lee, 2006, Id helix-loop-helix proteins negatively regulate TRANCE-mediated osteoclast differentiation., Blood, 107, 2686, 10.1182/blood-2005-07-2798 · DOI
  21. Kim, 2002, A novel member of the leukocyte receptor complex regulates osteoclast differentiation., J Exp Med, 195, 201, 10.1084/jem.20011681 · DOI
  22. Koga, 2004, Costimulatory signals mediated by the ITAM motif cooperate with RANKL for bone homeostasis., Nature, 428, 758, 10.1038/nature02444 · DOI
  23. Ishikawa, 2004, Involvement of FcRγ in signal transduction of osteoclast-associated receptor (OSCAR)., Int Immunol, 16, 1019, 10.1093/intimm/dxh103 · DOI
  24. Kobayashi, 2000, Tumor necrosis factor α stimulates osteoclast differentiation by a mechanism independent of the ODF/RANKL-RANK interaction., J Exp Med, 191, 275, 10.1084/jem.191.2.275 · DOI
  25. Suda, 2002, Lipopolysaccharide supports survival and fusion of preosteoclasts independent of TNF-α, IL-1, and RANKL., J Cell Physiol, 190, 101, 10.1002/jcp.10041 · DOI
  26. Fuller, 2006, Murine osteoclast formation and function: differential regulation by humoral agents., Endocrinology, 147, 1979, 10.1210/en.2005-1340 · DOI
  27. Kim, 2006, MCP-1-induced human osteoclast-like cells are tartrate-resistant acid phosphatase, NFATc1, and calcitonin receptor-positive but require receptor activator of NFκB ligand for bone resorption., J Biol Chem, 281, 1274, 10.1074/jbc.M510156200 · DOI
  28. McInnes, 1988, Interleukin 4 induces cultured monocytes/macrophages to form giant multinucleated cells., J Exp Med, 167, 598, 10.1084/jem.167.2.598 · DOI
  29. McNally, 1995, Interleukin-4 induces foreign body giant cells from human monocytes/macrophages. Differential lymphokine regulation of macrophage fusion leads to morphological variants of multinucleated giant cells., Am J Pathol, 147, 1487
  30. Kim, 2005, Osteoclast differentiation independent of the TRANCE-RANK-TRAF6 axis., J Exp Med, 202, 589, 10.1084/jem.20050978 · DOI
  31. Kukita, 2004, RANKL-induced DC-STAMP is essential for osteoclastogenesis., J Exp Med, 200, 941, 10.1084/jem.20040518 · DOI
  32. So, 2003, Microphthalmia transcription factor and PU. 1 synergistically induce the leukocyte receptor osteoclast-associated receptor gene expression., J Biol Chem, 278, 24209, 10.1074/jbc.M302940200 · DOI

Cited by

Metadata

Authors
Kim, K., Lee, S.-H., Ha Kim, J., Choi, Y., & Kim, N.
Affiliations
  • National Research Laboratory for Regulation of Bone Metabolism and Disease (K.K., J.H.K., N.K.), Medical Research Center for Gene Regulation, Chonnam National University Medical School, Gwangju 501-746, Korea
  • Department of Pathology and Laboratory Medicine (S.-H.L., Y.C.), University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19014
Venue
Molecular Endocrinology
Publisher
The Endocrine Society
Published
2008-01-01
Volume
22
Issue
1
Pages
176-185
ISSN
  • 0888-8809
  • 1944-9917
DOI
10.1210/me.2007-0237
Cited by
421
References
5
Language
en

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review

Data sources

Bibliographic data: Semantic Scholar, OpenAlex, Crossref.

Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).

Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.