MicroRNA regulation of the CUC genes is required for boundary size control in Arabidopsis meristems
Open accessAbstract
We have analysed the role of a microRNA, miR164, in boundary formation during organ initiation from Arabidopsis meristems. The establishment and maintenance of the boundary domain are controlled by three partially redundant genes, CUP-SHAPED COTYLEDON1 (CUC1), CUC2 and CUC3. We show that miR164 overexpression phenocopies the cuc1 cuc2 double mutant by inducing post-transcriptional downregulation of CUC1 and CUC2 but not CUC3 mRNA levels. Disruption of CUC2 regulation by miR164, either by making CUC2 resistant to the miRNA or by reducing miRNA levels leads to similar enlarged boundary domains. We relate this enlargement to the division patterns of the boundary cells. We propose that miR164 constrains the expansion of the boundary domain, by degrading CUC1 and CUC2 mRNAs.
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Cells in the sepal-to-meristem boundary show a reduced proliferation rate compared with those in the flower meristem.
“Similarly, cells in the sepal-to-meristem boundary show a reduced proliferation rate compared with those in the flower meristem (Laufs et al., 2004).”
From <i>Divide et impera</i>: boundaries shape the plant body and initiate new meristems (2016) · cites this paper as Laufs et al., 2004
CUC1 and CUC2 transcript accumulation is modulated by microRNA miR164.
“CUC1 and CUC2 transcript accumulation is modulated by the action of microRNA miR164 (Laufs et al., 2004).”
From <i>Divide et impera</i>: boundaries shape the plant body and initiate new meristems (2016) · cites this paper as Laufs et al., 2004
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Specific effects of microRNAs on the plant transcriptome
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<i>Divide et impera</i>: boundaries shape the plant body and initiate new meristems
Wang, Q., Hasson, A., Rossmann, S., & Theres, K. (2016) · New Phytologist · doi:10.1111/nph.13641 · Cited by 106 · see citing works
- Authors
- Laufs, P., Peaucelle, A., Morin, H., & Traas, J.
- Affiliations
- Institut Jean-Pierre Bourgin (FR)
- Venue
- Development
- Publisher
- The Company of Biologists
- Published
- 2004-09-01
- Volume
- 131
- Issue
- 17
- Pages
- 4311-4322
- ISSN
- 0950-1991 (ISSN-L)
- 1477-9129
- OpenAlex
- W2130900063
- Cited by
- 543
- References
- 4
- Citations by year
- 2026: 8 · 2025: 13 · 2024: 21 · 2023: 21 · 2022: 25 · 2021: 15 · 2020: 26 · 2019: 22 · 2018: 20 · 2017: 28 · 2016: 19 · 2015: 41 · 2014: 15 · 2013: 29 · 2012: 33
- FWCI
- 38.79
- Type
- journal article
- Language
- en
- Open access
- green
- hal.inrae.fr
- Licence
- other-oa
- Locations
- Development (publishedVersion)
- PubMed (publishedVersion)
- HAL (submittedVersion, other-oa)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Molecular Biology Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Developmental Biology and Gene Regulation
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › MicroRNA in disease regulation
- Keywords
- miR164, microRNA, CUC1, CUC2, organ initiation, Arabidopsis thaliana, meristem, post-transcriptional regulation, mRNA degradation, cell division patterns
- MeSH terms
- Base Sequence, Binding Sites, Cell Division, Histones, Models, Genetic, Molecular Sequence Data, Mutation, Phenotype, RNA, RNA Processing, Post-Transcriptional, RNA, Messenger, Arabidopsis, Protein Structure, Tertiary, DNA Primers, Gene Expression Regulation, Plant, Meristem, Microscopy, Confocal, Reverse Transcriptase Polymerase Chain Reaction, MicroRNAs
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