The DOI matches its Crossref record; 5 of the 5 references it cites were found in a registry or an index.
CD41+ cmyb+ precursors colonize the zebrafish pronephros by a novel migration route to initiate adult hematopoiesis
Open accessAbstract
Development of the vertebrate blood lineages is complex, with multiple waves of hematopoietic precursors arising in different embryonic locations. Monopotent, or primitive, precursors first give rise to embryonic macrophages or erythrocytes. Multipotent, or definitive, precursors are subsequently generated to produce the adult hematopoietic lineages. In both the zebrafish and the mouse, the first definitive precursors are committed erythromyeloid progenitors (EMPs) that lack lymphoid differentiation potential. We have previously shown that zebrafish EMPs arise in the posterior blood island independently from hematopoietic stem cells (HSCs). In this report, we demonstrate that a fourth wave of hematopoietic precursors arises slightly later in the zebrafish aorta/gonad/mesonephros (AGM) equivalent. We have identified and prospectively isolated these cells by CD41(itga2b) and cmyb expression. Unlike EMPs, CD41+ AGM cells colonize the thymus to generate rag2+ T lymphocyte precursors. Timelapse imaging and lineage tracing analyses demonstrate that AGM-derived precursors use a previously undescribed migration pathway along the pronephric tubules to initiate adult hematopoiesis in the developing kidney, the teleostean equivalent of mammalian bone marrow. Finally, we have analyzed the gene expression profiles of EMPs and AGM precursors to better understand the molecular cues that pattern the first definitive hematopoietic cells in the embryo. Together, these studies suggest that expression of CD41 and cmyb marks nascent HSCs in the zebrafish AGM, and provide the means to further dissect HSC generation and function in the early vertebrate embryo.
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Cites
Transplantation and in vivo imaging of multilineage engraftment in zebrafish bloodless mutants
Traver, D., Paw, B. H., Poss, K. D., Penberthy, W. T., Lin, S., & Zon, L. I. (2003) · Nature Immunology · doi:10.1038/ni1007 · Cited by 800
Self-citationTranscription Factors, Normal Myeloid Development, and Leukemia
Tenen, D. G., Hromas, R., Licht, J. D., & Zhang, D.-E. (1997) · Blood · doi:10.1182/blood.v90.2.489 · Cited by 735
Transcription factors in myeloid development: balancing differentiation with transformation
Rosenbauer, F., & Tenen, D. G. (2007) · Nature Reviews Immunology · doi:10.1038/nri2024 · Cited by 606 · cited 2×
“1 (spi1 – Zebrafish Information Network), transcription factors that act as master regulators of erythroid and myeloid fate commitment, respectively, in hematopoietic progenitor cells (Tenen et al., 1997; Miyamoto et al., 2002; Rosenbauer and Tenen, 2007).”
BackgroundResultMyeloid or Lymphoid Promiscuity as a Critical Step in Hematopoietic Lineage Commitment
Miyamoto, T., Iwasaki, H., Reizis, B., Ye, M., Graf, T., Weissman, I. L., & Akashi, K. (2002) · Developmental Cell · doi:10.1016/s1534-5807(02)00201-0 · Cited by 415
Direct regulation of Gata3 expression determines the T helper differentiation potential of Notch
Amsen, D., Antov, A., Jankovic, D., Sher, A., Radtke, F., Souabni, A., Busslinger, M., McCright, B., Gridley, T., & Flavell, R. A. (2007) · Immunity · doi:10.1016/j.immuni.2007.05.021 · Cited by 396
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Haematopoietic stem cells derive directly from aortic endothelium during development
Bertrand, J. Y., Chi, N. C., Santoso, B., Teng, S., Stainier, D. Y. R., & Traver, D. (2010) · Nature · doi:10.1038/nature08738 · Cited by 1,025
Self-citationIn vivo imaging of haematopoietic cells emerging from the mouse aortic endothelium
Boisset, J.-C., van Cappellen, W., Andrieu-Soler, C., Galjart, N., Dzierzak, E., & Robin, C. (2010) · Nature · doi:10.1038/nature08764 · Cited by 917
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Proinflammatory Signaling Regulates Hematopoietic Stem Cell Emergence
Espín-Palazón, R., Stachura, D. L., Campbell, C. A., García-Moreno, D., Del Cid, N., Kim, A. D., Candel, S., Meseguer, J., Mulero, V., & Traver, D. (2014) · Cell · doi:10.1016/j.cell.2014.10.031 · Cited by 333
Self-citationZebrafish erythropoiesis and the utility of fish as models of anemia
Kulkeaw, K., & Sugiyama, D. (2012) · Stem Cell Research & Therapy · doi:10.1186/scrt146 · Cited by 125
Rissone, A., Weinacht, K. G., la Marca, G., Bishop, K., Giocaliere, E., Jagadeesh, J., Felgentreff, K., Dobbs, K., Al-Herz, W., Jones, M., et al. (2015) · Journal of Experimental Medicine · doi:10.1084/jem.20141286 · Cited by 63
Imamura, S., Uchiyama, J., Koshimizu, E., Hanai, J.-I., Raftopoulou, C., Murphey, R. D., Bayliss, P. E., Imai, Y., Burns, C. E., Masutomi, K., et al. (2008) · PLoS ONE · doi:10.1371/journal.pone.0003364 · Cited by 58
Single-cell analyses reveal early thymic progenitors and pre-B cells in zebrafish
Rubin, S. A., Baron, C. S., Pessoa Rodrigues, C., Duran, M., Corbin, A. F., Yang, S. P., Trapnell, C., & Zon, L. I. (2022) · Journal of Experimental Medicine · doi:10.1084/jem.20220038 · Cited by 56
Using the Zebrafish as a Genetic Model to Study Erythropoiesis
Zhang, Y., Chen, M., & Chen, C. (2021) · International Journal of Molecular Sciences · doi:10.3390/ijms221910475 · Cited by 25
Metadata
- Authors
- Bertrand, J. Y., Kim, A. D., Teng, S., & Traver, D.
- Venue
- Development
- Publisher
- The Company of Biologists
- Published
- 2008-05-15
- Volume
- 135
- Issue
- 10
- Pages
- 1853-1862
- ISSN
- 0950-1991 (ISSN-L)
- 1477-9129
- OpenAlex
- W2106495713
- Cited by
- 214
- References
- 5
- FWCI
- 8.02
- Type
- journal article
- Language
- en
- Open access
- bronze
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