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VEGF is required for growth and survival in neonatal mice
Open accessAbstract
We employed two independent approaches to inactivate the angiogenic protein VEGF in newborn mice: inducible, Cre-loxP-mediated gene targeting, or administration of mFlt(1-3)-IgG, a soluble VEGF receptor chimeric protein. Partial inhibition of VEGF achieved by inducible gene targeting resulted in increased mortality, stunted body growth and impaired organ development, most notably of the liver. Administration of mFlt(1-3)-IgG, which achieves a higher degree of VEGF inhibition, resulted in nearly complete growth arrest and lethality. Ultrastructural analysis documented alterations in endothelial and other cell types. Histological and biochemical changes consistent with liver and renal failure were observed. Endothelial cells isolated from the liver of mFlt(1-3)-IgG-treated neonates demonstrated an increased apoptotic index, indicating that VEGF is required not only for proliferation but also for survival of endothelial cells. However, such treatment resulted in less significant alterations as the animal matured, and the dependence on VEGF was eventually lost some time after the fourth postnatal week. Administration of mFlt(1-3)-IgG to juvenile mice failed to induce apoptosis in liver endothelial cells. Thus, VEGF is essential for growth and survival in early postnatal life. However, in the fully developed animal, VEGF is likely to be involved primarily in active angiogenesis processes such as corpus luteum development.
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Cites
Heid, C. A., Stevens, J., Livak, K. J., & Williams, P. M. (1996) · Genome Research · doi:10.1101/gr.6.10.986 · Cited by 6,174
Risau, W. (1997) · Nature · doi:10.1038/386671a0 · Cited by 5,727
Endostatin: an endogenous inhibitor of angiogenesis and tumor growth
O'Reilly, M. S., Boehm, T., Shing, Y., Fukai, N., Vasios, G., Lane, W. S., Flynn, E., Birkhead, J. R., Olsen, B. R., & Folkman, J. (1997) · Cell · doi:10.1016/s0092-8674(00)81848-6 · Cited by 4,486
The Biology of Vascular Endothelial Growth Factor
Ferrara, N., & Davis-Smyth, T. (1997) · Endocrine Reviews · doi:10.1210/edrv.18.1.0287 · Cited by 4,330
Self-citationAbnormal blood vessel development and lethality in embryos lacking a single VEGF allele
Carmeliet, P., Ferreira, V., Breier, G., Pollefeyt, S., Kieckens, L., Gertsenstein, M., Fahrig, M., Vandenhoeck, A., Harpal, K., Eberhardt, C., et al. (1996) · Nature · doi:10.1038/380435a0 · Cited by 4,117
Failure of blood-island formation and vasculogenesis in Flk-1-deficient mice
Shalaby, F., Rossant, J., Yamaguchi, T. P., Gertsenstein, M., Wu, X.-F., Breitman, M. L., & Schuh, A. C. (1995) · Nature · doi:10.1038/376062a0 · Cited by 3,931
Heterozygous embryonic lethality induced by targeted inactivation of the VEGF gene
Ferrara, N., Carver-Moore, K., Chen, H., Dowd, M., Lu, L., O'Shea, K. S., Powell-Braxton, L., Hillan, K. J., & Moore, M. W. (1996) · Nature · doi:10.1038/380439a0 · Cited by 3,628
Self-citationO'Reilly, M. (1994) · Cell · doi:10.1016/0092-8674(94)90200-3 · Cited by 3,345
Role of the Flt-1 receptor tyrosine kinase in regulating the assembly of vascular endothelium
Fong, G.-H., Rossant, J., Gertsenstein, M., & Breitman, M. L. (1995) · Nature · doi:10.1038/376066a0 · Cited by 2,606
Role of insulin-like growth factors in embryonic and postnatal growth
Baker, J., Liu, J.-P., Robertson, E. J., & Efstratiadis, A. (1993) · Cell · doi:10.1016/s0092-8674(05)80085-6 · Cited by 2,463
Integrin αvβ3 antagonists promote tumor regression by inducing apoptosis of angiogenic blood vessels
Brooks, P. C., Montgomery, A. M. P., Rosenfeld, M., Reisfeld, R. A., Hu, T., Klier, G., & Cheresh, D. A. (1994) · Cell · doi:10.1016/0092-8674(94)90007-8 · Cited by 2,262
A novel method for real time quantitative RT-PCR.
Gibson, U. E., Heid, C. A., & Williams, P. M. (1996) · Genome Research · doi:10.1101/gr.6.10.995 · Cited by 2,075
Gerber, H.-P., McMurtrey, A., Kowalski, J., Yan, M., Keyt, B. A., Dixit, V., & Ferrara, N. (1998) · Journal of Biological Chemistry · doi:10.1074/jbc.273.46.30336 · Cited by 1,981
Self-citationInducible Gene Targeting in Mice
Kühn, R., Schwenk, F., Aguet, M., & Rajewsky, K. (1995) · Science · doi:10.1126/science.7660125 · Cited by 1,641
Self-citationPresta, L. G., Chen, H., O'Connor, S. J., Chisholm, V., Meng, Y. G., KRUMMEN, L. A., Winkler, M., & Ferrara, N. (1997) · PubMed · Cited by 1,608
Self-citationAlon, T., Hemo, I., Itin, A., Pe'er, J., Stone, J., & Keshet, E. (1995) · Nature Medicine · doi:10.1038/nm1095-1024 · Cited by 1,604
Immune Response in Mice that Lack the Interferon-γ Receptor
Huang, S., Hendriks, W., Althage, A., Hemmi, S., Bluethmann, H., Kamijo, R., Vilček, J., Zinkernagel, R. M., & Aguet, M. (1993) · Science · doi:10.1126/science.8456301 · Cited by 1,548
Self-citationDeletion of a DNA Polymerase β Gene Segment in T Cells Using Cell Type-Specific Gene Targeting
Gu, H., Marth, J. D., Orban, P. C., Mossmann, H., & Rajewsky, K. (1994) · Science · doi:10.1126/science.8016642 · Cited by 1,397
Benjamin, L. E., Hemo, I., & Keshet, E. (1998) · Development · doi:10.1242/dev.125.9.1591 · Cited by 1,265
The Physiological Basis of Medical Practice.
(1950) · Archives of Internal Medicine · doi:10.1001/archinte.1950.00230140152019 · Cited by 1,179
Erythropoietin: structure, control of production, and function
Jelkmann, W. (1992) · Physiological Reviews · doi:10.1152/physrev.1992.72.2.449 · Cited by 1,151
Hiratsuka, S., Minowa, O., Kuno, J., Noda, T., & Shibuya, M. (1998) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.95.16.9349 · Cited by 1,062
Good, D. J., Polverini, P. J., Rastinejad, F., Le Beau, M. M., Lemons, R. S., Frazier, W. A., & Bouck, N. P. (1990) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.87.17.6624 · Cited by 1,054
Roberts, W. G., & Palade, G. E. (1995) · Journal of Cell Science · doi:10.1242/jcs.108.6.2369 · Cited by 1,033
Gerber, H.-P., Dixit, V., & Ferrara, N. (1998) · Journal of Biological Chemistry · doi:10.1074/jbc.273.21.13313 · Cited by 981
Self-citationA Requirement for Flk1 in Primitive and Definitive Hematopoiesis and Vasculogenesis
Shalaby, F., Ho, J., Stanford, W. L., Fischer, K.-D., Schuh, A. C., Schwartz, L., Bernstein, A., & Rossant, J. (1997) · Cell · doi:10.1016/s0092-8674(00)80283-4 · Cited by 911
Pericytes in the microvasculature
Hirschi, K. (1996) · Cardiovascular Research · doi:10.1016/0008-6363(96)00063-6 · Cited by 775
Quinn, T. P., Peters, K. G., De Vries, C., Ferrara, N., & Williams, L. T. (1993) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.90.16.7533 · Cited by 754
Self-citationYuan, F., Chen, Y., Dellian, M., Safabakhsh, N., Ferrara, N., & Jain, R. K. (1996) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.93.25.14765 · Cited by 719
Self-citationVascular Endothelial Growth Factor Induces Endothelial Fenestrations In Vitro
Esser, S., Wolburg, K., Wolburg, H., Breier, G., Kurzchalia, T., & Risau, W. (1998) · The Journal of Cell Biology · doi:10.1083/jcb.140.4.947 · Cited by 648
Vascular endothelial growth factor is essential for corpus luteum angiogenesis
Ferrara, N., Chen, H., Davis-Smyth, T., Gerber, H.-P., Nguyen, T.-N., Peers, D., Chisholm, V., Hillan, K. J., & Schwall, R. H. (1998) · Nature Medicine · doi:10.1038/nm0398-336 · Cited by 624
Self-citationDifferentiation of endothelium
Risau, W. (1995) · The FASEB Journal · doi:10.1096/fasebj.9.10.7615161 · Cited by 607
Benjamin, L. E., & Keshet, E. (1997) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.94.16.8761 · Cited by 506
Keyt, B. A., Nguyen, H. V., Berleau, L. T., Duarte, C. M., Park, J., Chen, H., & Ferrara, N. (1996) · Journal of Biological Chemistry · doi:10.1074/jbc.271.10.5638 · Cited by 497
Self-citationExpression of vascular permeability factor/vascular endothelial growth factor in normal rat tissues
Monacci, W. T., Merrill, M. J., & Oldfield, E. H. (1993) · American Journal of Physiology-Cell Physiology · doi:10.1152/ajpcell.1993.264.4.c995 · Cited by 370
The 16-kilodalton N-terminal fragment of human prolactin is a potent inhibitor of angiogenesis
Clapp, C., MARTIAL, J. A., Guzman, R. C., Rentier-Delure, F., & Weiner, R. I. (1993) · Endocrinology · doi:10.1210/en.133.3.1292 · Cited by 340
Katoh, O., Tauchi, H., Kawaishi, K., Kimura, A., & Satow, Y. (1995) · PubMed · Cited by 303
Vecchi, A., Garlanda, C., Lampugnani, M. G., Resnati, M., Matteucci, C., Stoppacciaro, A., Schnürch, H., Risau, W., Ruco, L., & Mantovani, A. (1994) · PubMed · Cited by 301
Kitamoto, Y., Tokunaga, H., & Tomita, K. (1997) · Journal of Clinical Investigation · doi:10.1172/jci119416 · Cited by 294
Borgström, P., Bourdon, M. A., Hillan, K. J., Sriramarao, P., & Ferrara, N. (1998) · The Prostate · Cited by 248
Self-citationThe versatility of microvascular pericytes: from mesenchyme to smooth muscle?
Nehls, V., & Drenckhahn, D. (1993) · Histochemistry · doi:10.1007/bf00268014 · Cited by 214
Davis-Smyth, T., Chen, H., Park, J., Presta, L. G., & Ferrara, N. (1996) · The EMBO Journal · doi:10.1002/j.1460-2075.1996.tb00872.x · Cited by 200
Self-citationAugustin, H. G., Braun, K., Telemenakis, I., Modlich, U., & Kühn, W. (1995) · Europe PMC (PubMed Central) · Cited by 186
Chapter 15 Simultaneous Measurement of Cell Cycle and Apoptotic Cell Death
Moore, A., Donahue, C. J., Bauer, K. D., & Mather, J. P. (1998) · Methods in cell biology · doi:10.1016/s0091-679x(08)61584-8 · Cited by 141
Vascular development and disorders: Molecular analysis and pathogenic insights
Carmeliet, P., & Collen, D. (1998) · Kidney International · doi:10.1046/j.1523-1755.1998.00936.x · Cited by 114
Myeloid progenitor cell regulatory effects of vascular endothelial cell growth factor.
Broxmeyer (1995) · International Journal of Hematology · doi:10.1016/0925-5710(95)00412-2 · Cited by 112
Sage, E. H., Bassuk, J. A., Yost, J. C., Folkman, M. J., & Lane, T. F. (1995) · Journal of Cellular Biochemistry · doi:10.1002/jcb.240570113 · Cited by 65
Cited by
The biology of VEGF and its receptors
Ferrara, N., Gerber, H.-P., & LeCouter, J. (2003) · Nature Medicine · doi:10.1038/nm0603-669 · Cited by 9,591
Self-citationVascular-specific growth factors and blood vessel formation
Yancopoulos, G. D., Davis, S., Gale, N. W., Rudge, J. S., Wiegand, S. J., & Holash, J. (2000) · Nature · doi:10.1038/35025215 · Cited by 3,699
VEGF receptor signalling ? in control of vascular function
Olsson, A.-K., Dimberg, A., Kreuger, J., & Claesson-Welsh, L. (2006) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm1911 · Cited by 3,122
CCL2 recruits inflammatory monocytes to facilitate breast-tumour metastasis
Qian, B.-Z., Li, J., Zhang, H., Kitamura, T., Zhang, J., Campion, L. R., Kaiser, E. A., Snyder, L. A., & Pollard, J. W. (2011) · Nature · doi:10.1038/nature10138 · Cited by 2,874
VEGF in signaling and disease: beyond discovery and development
Apte, R. S., Chen, D. S., & Ferrara, N. (2019) · Cell · doi:10.1016/j.cell.2019.01.021 · Cited by 2,721
Self-citation
Metadata
- Authors
- Gerber, H.-P., Hillan, K. J., Ryan, A. M., Kowalski, J., Keller, G.-A., Rangell, L., Wright, B. D., Radtke, F., Aguet, M., & Ferrara, N.
- Venue
- Development
- Publisher
- The Company of Biologists
- Published
- 1999-03-15
- Volume
- 126
- Issue
- 6
- Pages
- 1149-1159
- ISSN
- 0950-1991 (ISSN-L)
- 1477-9129
- OpenAlex
- W2138454311
- Cited by
- 915
- References
- 47
- FWCI
- 15.01
- Type
- journal article
- Language
- en
- Open access
- green
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