Development-on-chip: in vitro neural tube patterning with a microfluidic device
Open accessAbstract
Embryogenesis is a highly regulated process in which the precise spatial and temporal release of soluble cues directs differentiation of multipotent stem cells into discrete populations of specialized adult cell types. In the spinal cord, neural progenitor cells are directed to differentiate into adult neurons through the action of mediators released from nearby organizing centers, such as the floor plate and paraxial mesoderm. These signals combine to create spatiotemporal diffusional landscapes that precisely regulate the development of the central nervous system (CNS). Currently, in vivo and ex vivo studies of these signaling factors present some inherent ambiguity. In vitro methods are preferred for their enhanced experimental clarity but often lack the technical sophistication required for biological realism. In this article, we present a versatile microfluidic platform capable of mimicking the spatial and temporal chemical environments found in vivo during neural tube development. Simultaneous opposing and/or orthogonal gradients of developmental morphogens can be maintained, resulting in neural tube patterning analogous to that observed in vivo.
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Microfluidic systems can create miniaturized niches with precise control over mechano-chemical properties.
“microfluidic systems can be leveraged to create miniaturized niches with precise control over mechano-chemical properties91.”
From Organoids (2022) · cites this paper as [91]
The Results (Microdevice design, fabrication and characterization; page not provided) describe a miniaturized, three-dimensional cell-culture chamber, while the Discussion describes a user-defined chemical concentration landscape. The paper does not show precise control over mechanical properties, so the mechano-chemical part of the claim is only partly supported. (LLM, conf=0.85)
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- Authors
- Demers, C. J., Soundararajan, P., Chennampally, P., Cox, G. A., Briscoe, J., Collins, S. D., & Smith, R. L.
- Affiliations
- National Institute for Medical Research (GB)
- Moffitt Cancer Center (US)
- University of Maine (US)
- Jackson Laboratory (US)
- Corresponding author
- Collins, Scott D.
- Venue
- Development
- Publisher
- The Company of Biologists
- Published
- 2016-06-01
- Volume
- 143
- Issue
- 11
- Pages
- 1884-1892
- ISSN
- 0950-1991 (ISSN-L)
- 1477-9129
- PMC
- PMC4920155
- OpenAlex
- W2411867197
- Cited by
- 149
- References
- 47
- Citations by year
- 2026: 13 · 2025: 12 · 2024: 15 · 2023: 23 · 2022: 25 · 2021: 22 · 2020: 15 · 2019: 16 · 2018: 4 · 2017: 3 · 2016: 1
- FWCI
- 4.16
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Locations
- Development (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- Figshare (submittedVersion, cc-by)
- The Mouseion at the JAXlibrary (Jackson Laboratory) (submittedVersion)
- HighWire Press Open Archive (submittedVersion)
- Funders
- National Science Foundation (1145949)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Developmental Biology and Gene Regulation
- Physical Sciences › Engineering › Biomedical Engineering › Microfluidic and Capillary Electrophoresis Applications
- Life Sciences › Neuroscience › Developmental Neuroscience › Neurogenesis and neuroplasticity mechanisms
- Keywords
- neural tube patterning, microfluidics, embryonic development, morphogen gradients, floor plate, paraxial mesoderm, neural progenitor cells, spinal cord development, central nervous system development, in vitro development
- MeSH terms
- Animals, Cell Differentiation, Computer Simulation, Equipment Design, Mice, Transgenic, Motor Neurons, Time Factors, Transcription Factors, Body Patterning, Neural Tube, Lab-On-A-Chip Devices, Time-Lapse Imaging
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