The DOI matches its Crossref record; 3 of the 3 references it cites were found in a registry or an index.
ClearSee: a rapid optical clearing reagent for whole-plant fluorescence imaging
Open accessAbstract
Imaging techniques for visualizing and analyzing precise morphology and gene expression patterns are essential for understanding biological processes during development in all organisms. With the aid of chemical screening, we developed a clearing method using chemical solutions, termed ClearSee, for deep imaging of morphology and gene expression in plant tissues. ClearSee rapidly diminishes chlorophyll autofluorescence while maintaining fluorescent protein stability. By adjusting the refractive index mismatch, whole-organ and plant imaging can be performed both by confocal and two-photon excitation microscopy in ClearSee-treated samples. Moreover, ClearSee is applicable to multicolor imaging of fluorescent proteins to allow structural analysis of multiple gene expression. Given that ClearSee is compatible with staining by chemical dyes, the technique is useful for deep imaging in conjunction with genetic markers and for plant species not amenable to transgenic approaches. This method is useful for whole imaging for intact morphology and will help to accelerate the discovery of new phenomena in plant biological research.
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Structural and molecular interrogation of intact biological systems
Chung, K., Wallace, J., Kim, S.-Y., Kalyanasundaram, S., Andalman, A. S., Davidson, T. J., Mirzabekov, J. J., Zalocusky, K. A., Mattis, J., Denisin, A. K., et al. (2013) · Nature · doi:10.1038/nature12107 · Cited by 2,008
Micheva, K. D., & Smith, S. J. (2007) · Neuron · doi:10.1016/j.neuron.2007.06.014 · Cited by 787
CHLOROPLAST UNUSUAL POSITIONING1 Is Essential for Proper Chloroplast Positioning
Oikawa, K., Kasahara, M., Kiyosue, T., Kagawa, T., Suetsugu, N., Takahashi, F., Kanegae, T., Niwa, Y., Kadota, A., & Wada, M. (2003) · The Plant Cell · doi:10.1105/tpc.016428 · Cited by 258
Cited by
High levels of auxin signalling define the stem-cell organizer of the vascular cambium
Smetana, O., Mäkilä, R., Lyu, M., Amiryousefi, A., Sánchez Rodríguez, F., Wu, M.-F., Solé-Gil, A., Leal Gavarrón, M., Siligato, R., Miyashima, S., et al. (2019) · Nature · doi:10.1038/s41586-018-0837-0 · Cited by 336
Local Auxin Biosynthesis Is a Key Regulator of Plant Development
Brumos, J., Robles, L. M., Yun, J., Vu, T. C., Jackson, S., Alonso, J. M., & Stepanova, A. N. (2018) · Developmental Cell · doi:10.1016/j.devcel.2018.09.022 · Cited by 323
Nguyen, C. T., Kurenda, A., Stolz, S., Chételat, A., & Farmer, E. E. (2018) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1807049115 · Cited by 294
Decaestecker, W., Buono, R. A., Pfeiffer, M. L., Vangheluwe, N., Jourquin, J., Karimi, M., Van Isterdael, G., Beeckman, T., Nowack, M. K., & Jacobs, T. B. (2019) · The Plant Cell · doi:10.1105/tpc.19.00454 · Cited by 258
ARF degradation defines a deeply conserved step in auxin response
de Roij, M., Hernández García, J., Das, S., Borst, J. W., & Weijers, D. (2025) · Nature Plants · doi:10.1038/s41477-025-01975-1 · Cited by 16
Metadata
- Authors
- Kurihara, D., Mizuta, Y., Sato, Y., & Higashiyama, T.
- Venue
- Development
- Publisher
- The Company of Biologists
- Published
- 2015-01-01
- Volume
- 142
- Issue
- 23
- Pages
- 4168-79
- ISSN
- 0950-1991 (ISSN-L)
- 1477-9129
- OpenAlex
- W2228103413
- Cited by
- 602
- References
- 3
- FWCI
- 12.89
- Type
- journal article
- Language
- en
- Open access
- hybrid
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