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Cited by 594

The DOI matches its Crossref record; 48 of the 48 references it cites were found in a registry or an index.

The zinc-finger transcription factor Klf4 is required for terminal differentiation of goblet cells in the colon

Open access
Katz, Jonathan P., Perreault, Nathalie, Goldstein, Bree G., Lee, Catherine S., Labosky, Patricia A., Yang, Vincent W., Kaestner, Klaus H.·2002·Development
DOI 10.1242/dev.129.11.2619
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Abstract

Klf4 (formerly GKLF) is a zinc-finger transcription factor expressed in the epithelia of the skin, lungs, gastrointestinal tract and several other organs. In vitro studies have suggested that Klf4 plays an important role in cell proliferation and/or differentiation. Mice homozygous for a null mutation in Klf4 die within 15 hours of birth and show selective perturbation of late-stage differentiation structures in the epidermis, but the function of Klf4 in the gastrointestinal tract has not been investigated. To address this issue, we have generated Klf4–/– mice by homologous recombination in embryonic stem cells. In this study, we provide the first in vivo evidence that Klf4 is a goblet cell-specific differentiation factor in the colon. Klf4–/– mice exhibit normal cell proliferation and cell death rates in the colon on postnatal day 1. However, Klf4–/– mice demonstrate a 90% decrease in the number of goblet cells in the colon, show abnormal expression of the goblet cell-specific marker Muc2 by in situ hybridization, have abnormal staining of the colonic epithelium with Alcian Blue for acidic mucins, and lack normal goblet cell morphology by ultrastructural analysis. All other epithelial cell types are present in the colon of Klf4–/– mice. In summary, Klf4 plays a crucial role in colonic epithelial cell differentiation in vivo.

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Metadata

Authors
Katz, J. P., Perreault, N., Goldstein, B. G., Lee, C. S., Labosky, P. A., Yang, V. W., & Kaestner, K. H.
Venue
Development
Publisher
The Company of Biologists
Published
2002-06-01
Volume
129
Issue
11
Pages
2619-2628
ISSN
  • 0950-1991 (ISSN-L)
  • 1477-9129
DOI
10.1242/dev.129.11.2619
OpenAlex
W2106896953
Cited by
594
References
48
FWCI
6.16
Type
journal article
Language
en
Open access
green

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