Asialoglycoprotein receptor 1 is a specific cell-surface marker for isolating hepatocytes derived from human pluripotent stem cells
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Hepatocyte-like cells (HLCs) are derived from human pluripotent stem cells (hPSCs) in vitro, but differentiation protocols commonly give rise to a heterogeneous mixture of cells. This variability confounds the evaluation of in vitro functional assays performed using HLCs. Increased differentiation efficiency and more accurate approximation of the in vivo hepatocyte gene expression profile would improve the utility of hPSCs. Towards this goal, we demonstrate the purification of a subpopulation of functional HLCs using the hepatocyte surface marker asialoglycoprotein receptor 1 (ASGR1). We analyzed the expression profile of ASGR1-positive cells by microarray, and tested their ability to perform mature hepatocyte functions (albumin and urea secretion, cytochrome activity). By these measures, ASGR1-positive HLCs are enriched for the gene expression profile and functional characteristics of primary hepatocytes compared with unsorted HLCs. We have demonstrated that ASGR1-positive sorting isolates a functional subpopulation of HLCs from among the heterogeneous cellular population produced by directed differentiation.
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The differentiation protocol used was developed and optimized from published literature and chosen for its capacity to generate iHLOs with high CYP enzyme activity, particularly CYP3A4.
“The differentiation protocol utilized was developed and optimized from the published literatures18–21, specifically chosen for its capacity to generate iHLOs with high CYP enzyme activity, particularly CYP3A4, which is crucial for drug metabolism and toxicology evaluations.”
From Temporal proteomic profiling of iPSC-derived human liver organoids reveals optimal maturation for drug metabolism and toxicology (2026) · cites this paper as [20]
The Results and Discussion (page unavailable) describe an optimized differentiation protocol based on established methods. They also report increased CYP3A4 activity after ASGR1-positive HLCs were sorted and replated, compared with unsorted HLCs. The paper studies HLCs, not iHLOs, and does not say the differentiation protocol was chosen for its capacity to generate iHLOs with high CYP3A4 activity. (LLM, conf=0.88)
Experimental — accuracy not yet measuredThe protocol was chosen due to its demonstrated capacity to generate iHLOs with high CYP enzyme activity, particularly CYP3A4.
“This protocol was chosen due to its demonstrated capacity to generate iHLOs with high CYP enzyme activity, particularly CYP3A4, which is crucial for our subsequent compound or drug metabolism and toxicology evaluations.”
From Temporal proteomic profiling of iPSC-derived human liver organoids reveals optimal maturation for drug metabolism and toxicology (2026) · cites this paper as [20]
In Results and Discussion (page unavailable), the paper reports significantly higher CYP3A4 activity in ASGR1-enriched, replated hepatocyte-like cells than in unsorted cells. It studies hepatocyte-like cells, not iHLOs, and reports that primary human hepatocytes had substantially higher activity; it therefore does not establish high CYP3A4 activity in iHLOs generated by the chosen protocol. (LLM, conf=0.88)
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Subramanian, A., Tamayo, P., Mootha, V. K., Mukherjee, S., Ebert, B. L., Gillette, M. A., Paulovich, A., Pomeroy, S. L., Golub, T. R., Lander, E. S., et al. (2005) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0506580102 · Cited by 53,625 · see citing works
affy—analysis of Affymetrix GeneChip data at the probe level
Gautier, L., Cope, L., Bolstad, B. M., & Irizarry, R. A. (2004) · Bioinformatics · doi:10.1093/bioinformatics/btg405 · Cited by 5,221 · see citing works
A gene atlas of the mouse and human protein-encoding transcriptomes
Su, A. I., Wiltshire, T., Batalov, S., Lapp, H., Ching, K. A., Block, D., Zhang, J., Soden, R., Hayakawa, M., Kreiman, G., et al. (2004) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0400782101 · Cited by 3,475 · see citing works
Knowles, B. B., Howe, C. C., & Aden, D. P. (1980) · Science · doi:10.1126/science.6248960 · Cited by 2,026 · see citing works
Generation of Functional Human Pancreatic β Cells In Vitro
Pagliuca, F. W., Millman, J. R., Gürtler, M., Segel, M., Van Dervort, A., Ryu, J. H., Peterson, Q. P., Greiner, D., & Melton, D. A. (2014) · Cell · doi:10.1016/j.cell.2014.09.040 · Cited by 2,025 · see citing works
Mi, H., Muruganujan, A., & Thomas, P. D. (2012) · Nucleic Acids Research · doi:10.1093/nar/gks1118 · Cited by 1,693 · see citing works
Highly efficient generation of human hepatocyte-like cells from induced pluripotent stem cells
Si-Tayeb, K., Noto, F. K., Nagaoka, M., Li, J., Battle, M. A., Duris, C., North, P. E., Dalton, S., & Duncan, S. A. (2010) · Hepatology · doi:10.1002/hep.23354 · Cited by 1,282 · see citing works
Microscale culture of human liver cells for drug development
Khetani, S. R., & Bhatia, S. N. (2008) · Nature Biotechnology · doi:10.1038/nbt1361 · Cited by 1,219 · see citing works
Ashwell, G., & Morell, A. G. (1974) · Advances in enzymology and related areas of molecular biology/Advances in enzymology and related subjects · doi:10.1002/9780470122860.ch3 · Cited by 1,212 · see citing works
Bock, C., Kiskinis, E., Verstappen, G., Gu, H., Boulting, G., Smith, Z. D., Ziller, M., Croft, G. F., Amoroso, M. W., Oakley, D. H., et al. (2011) · Cell · doi:10.1016/j.cell.2010.12.032 · Cited by 1,009 · see citing works
Derivation of Embryonic Stem-Cell Lines from Human Blastocysts
Cowan, C. A., Klimanskaya, I. V., McMahon, J. A., Atienza, J., Witmyer, J., Zucker, J. P., Wang, S., Morton, C. C., McMahon, A. P., Powers, D., et al. (2004) · New England Journal of Medicine · doi:10.1056/nejmsr040330 · Cited by 964 · see citing works
Self-citationMarked differences in differentiation propensity among human embryonic stem cell lines
Osafune, K., Caron, L., Borowiak, M., Martinez, R. J., Fitzgerald, C., Sato, Y., Cowan, C. A., Chien, K. R., & Melton, D. A. (2008) · Nature Biotechnology · doi:10.1038/nbt1383 · Cited by 815 · see citing works
Self-citationDifferentiation and Transplantation of Human Embryonic Stem Cell–Derived Hepatocytes
Basma, H., Soto–Gutiérrez, A., Yannam, G. R., Liu, L., Ito, R., Yamamoto, T., Ellis, E. C. S., Carson, S. D., Sato, S., Chen, Y., et al. (2008) · Gastroenterology · doi:10.1053/j.gastro.2008.10.047 · Cited by 509 · see citing works
Donor-dependent variations in hepatic differentiation from human-induced pluripotent stem cells
Kajiwara, M., Aoi, T., Okita, K., Takahashi, R., Inoue, H., Takayama, N., Endô, H., Eto, K., Toguchida, J., Üemoto, S., et al. (2012) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1209979109 · Cited by 321 · see citing works
Pluripotent stem cell-derived hepatocyte-like cells
Schwartz, R. E., Fleming, H. E., Khetani, S. R., & Bhatia, S. N. (2014) · Biotechnology Advances · doi:10.1016/j.biotechadv.2014.01.003 · Cited by 281 · see citing works
HNF4A is essential for specification of hepatic progenitors from human pluripotent stem cells
DeLaForest, A., Nagaoka, M., Si-Tayeb, K., Noto, F. K., Konopka, G., Battle, M. A., & Duncan, S. A. (2011) · Development · doi:10.1242/dev.062547 · Cited by 219 · see citing works
Takayama, K., Morisaki, Y., Kuno, S., Nagamoto, Y., Harada, K., Furukawa, N., Ohtaka, M., Nishimura, K., Imagawa, K., Sakurai, F., et al. (2014) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1413481111 · Cited by 193 · see citing works
Differentiation of Hepatocytes from Pluripotent Stem Cells
Mallanna, S. K., & Duncan, S. A. (2013) · Current Protocols in Stem Cell Biology · doi:10.1002/9780470151808.sc01g04s26 · Cited by 121 · see citing works
Identification and quantification of the rat hepatocyte asialoglycoprotein receptor.
Schwartz, A. L., Marshak‐Rothstein, A., Rup, D., & Lodish, H. F. (1981) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.78.6.3348 · Cited by 91 · see citing works
Li, J., Chen, L., Zhang, X., Zhang, Y., Liu, H., Sun, B., Zhao, L., Ge, N., Qian, H., Yang, Y., et al. (2014) · PLoS ONE · doi:10.1371/journal.pone.0096185 · Cited by 66 · see citing works
Network Analysis of Primary Hepatocyte Dedifferentiation Using a Shotgun Proteomics Approach
Rowe, C., Goldring, C. E. P., Kitteringham, N. R., Jenkins, R. E., Lane, B. S., Sanderson, C., Elliott, V., Platt, V., Metcalfe, P., & Park, B. K. (2010) · Journal of Proteome Research · doi:10.1021/pr1001687 · Cited by 51 · see citing works
VeryGene: linking tissue-specific genes to diseases, drugs, and beyond for knowledge discovery
Yang, X., Ye, Y., Wang, G., Huang, H., Yu, D., & Liang, S. (2011) · Physiological Genomics · doi:10.1152/physiolgenomics.00178.2010 · Cited by 31 · see citing works
Isolation and primary culture of adult human hepatocytes
Miyazaki, K., Takaki, R., Nakayama, F., Yamauchi, S., Koga, A., & Todo, S. (1981) · Cell and Tissue Research · doi:10.1007/bf00210087 · Cited by 29 · see citing works
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Sorted by citations
Single-cell transcriptomic analyses provide insights into the developmental origins of neuroblastoma
Jansky, S., Sharma, A. K., Körber, V., Quintero, A., Toprak, U. H., Wecht, E. M., Gartlgruber, M., Greco, A., Chomsky, E., Grünewald, T. G. P., et al. (2021) · Nature Genetics · doi:10.1038/s41588-021-00806-1 · Cited by 311 · see citing works
Musunuru, K., Sheikh, F., Gupta, R. M., Houser, S. R., Maher, K. O., Milan, D. J., Terzic, A., & Wu, J. C. (2018) · Circulation Genomic and Precision Medicine · doi:10.1161/hcg.0000000000000043 · Cited by 230 · see citing works
Self-citationWarren, C. R., O’Sullivan, J. F., Friesen, M., Becker, C. E., Zhang, X., Liu, P., Wakabayashi, Y., Morningstar, J. E., Shi, X., Choi, J., et al. (2017) · Cell stem cell · doi:10.1016/j.stem.2017.01.010 · Cited by 191 · see citing works
Self-citationScalable production of tissue-like vascularized liver organoids from human PSCs
Harrison, S. P., Siller, R., Tanaka, Y., Chollet, M. E., de la Morena-Barrio, M. E., Xiang, Y., Patterson, B., Andersen, E., Bravo-Pérez, C., Kempf, H., et al. (2023) · Experimental & Molecular Medicine · doi:10.1038/s12276-023-01074-1 · Cited by 119 · see citing works
Couto, N. A. S., Al‐Majdoub, Z. M., Achour, B., Wright, P. C., Rostami‐Hodjegan, A., & Barber, J. (2019) · Molecular Pharmaceutics · doi:10.1021/acs.molpharmaceut.8b00941 · Cited by 94 · see citing works
Novel strategies to target proprotein convertase subtilisin kexin 9: beyond monoclonal antibodies
Seidah, N. G., Prat, A., Pirillo, A., Catapano, A. L., & Norata, G. D. (2019) · Cardiovascular Research · doi:10.1093/cvr/cvz003 · Cited by 89 · see citing works
Franco, M. E., Sutherland, G. E., Fernández-Luna, M. T., & Lavado, R. (2019) · Toxicology · doi:10.1016/j.tox.2019.152339 · Cited by 73 · see citing works
Hanaoka, K., Ikeno, T., Iwaki, S., Deguchi, S., Takayama, K., Mizuguchi, H., Tao, F., Kojima, N., Ohno, H., Sasaki, E., et al. (2024) · Science Advances · doi:10.1126/sciadv.adi8847 · Cited by 70 · see citing works
New Pharmacological Approaches to Target PCSK9
Catapano, A. L., Pirillo, A., & Norata, G. D. (2020) · Current Atherosclerosis Reports · doi:10.1007/s11883-020-00847-7 · Cited by 61 · see citing works
Biomaterials used in stem cell therapy for spinal cord injury
Higuchi, A., Kumar, S. S., Benelli, G., Ling, Q., Li, H.-F., Alarfaj, A. A., Munusamy, M. A., Sung, T., Chang, Y., & Murugan, K. (2019) · Progress in Materials Science · doi:10.1016/j.pmatsci.2019.02.002 · Cited by 59 · see citing works
Goulart, E., de Caires-Junior, L. C., Telles-Silva, K. A., Araújo, B. H. S., Kobayashi, G. S., Musso, C. M., Assoni, A. F., Oliveira, D., Caldini, E. T. E. G., Gerstenhaber, J. A., et al. (2019) · Stem Cell Research & Therapy · doi:10.1186/s13287-019-1367-x · Cited by 57 · see citing works
Graffmann, N., Scherer, B., & Adjaye, J. (2022) · Stem Cell Research · doi:10.1016/j.scr.2022.102763 · Cited by 52 · see citing works
Achour, B., Feteisi, H. A., Lanucara, F., Rostami‐Hodjegan, A., & Barber, J. (2017) · Drug Metabolism and Disposition · doi:10.1124/dmd.116.074732 · Cited by 50 · see citing works
Gurevich, I., Burton, S., Munn, C. G., Ohshima, M., Goedland, M., Czysz, K., & Rajesh, D. (2020) · Biology Open · doi:10.1242/bio.055087 · Cited by 47 · see citing works
Mallanna, S. K., Cayo, M. A., Twaroski, K., Gundry, R. L., & Duncan, S. A. (2016) · Stem Cell Reports · doi:10.1016/j.stemcr.2016.07.016 · Cited by 44 · see citing works
Miotto, P. M., Yang, C.-H., Keenan, S. N., Nardo, W. D., Beddows, C. A., Fidelito, G., Dodd, G. T., Parker, B. L., Hill, A. F., Burton, P. R., et al. (2024) · Nature Metabolism · doi:10.1038/s42255-023-00971-z · Cited by 39 · see citing works
Govarthanan, K., Vidyasekar, P., Gupta, P. K., Lenka, N., & Verma, R. S. (2020) · Cytotherapy · doi:10.1016/j.jcyt.2019.12.007 · Cited by 33 · see citing works
Clec10a regulates mite-induced dermatitis
Kanemaru, K., Noguchi, E., Tahara-Hanaoka, S., Mizuno, S., Tateno, H., Denda-Nagai, K., Irimura, T., Matsuda, H., Sugiyama, F., Takahashi, S., et al. (2019) · Science immunology · doi:10.1126/sciimmunol.aax6908 · Cited by 29 · see citing works
Humanity in a Dish: iPSC Cohort Studies
Warren, C. R., & Cowan, C. (2017) · Trends in Cell Biology · doi:10.1016/j.tcb.2017.09.006 · Cited by 30 · see citing works
Self-citationRaasch, M., Fritsche, E., Kurtz, A., Bauer, M. W., & Mosig, A. S. (2018) · Advanced Drug Delivery Reviews · doi:10.1016/j.addr.2018.06.008 · Cited by 30 · see citing works
Advancements in Disease Modeling and Drug Discovery Using iPSC-Derived Hepatocyte-like Cells
Blaszkiewicz, J., & Duncan, S. A. (2022) · Genes · doi:10.3390/genes13040573 · Cited by 28 · see citing works
Holmgren, G., Ulfenborg, B., Asplund, A., Toet, K. H., Andersson, C. X., Hammarstedt, A., Hanemaaijer, R., Küppers-Munther, B., & Synnergren, J. (2020) · International Journal of Molecular Sciences · doi:10.3390/ijms21020469 · Cited by 24 · see citing works
3D culture of functional human iPSC-derived hepatocytes using a core-shell microfiber
Nagata, S., Ozawa, F., Nie, M., & Takeuchi, S. (2020) · PLoS ONE · doi:10.1371/journal.pone.0234441 · Cited by 24 · see citing works
Nakai, S., Shibata, I., Shitamichi, T., Yamaguchi, H., Takagi, N., Inoue, T., Nakagawa, T., Kiyokawa, J., Wakabayashi, S., Miyoshi, T., et al. (2019) · Biology Open · doi:10.1242/bio.042192 · Cited by 23 · see citing works
Perrot, N., Pelletier, W., Bourgault, J., Couture, C., Li, Z., Mitchell, P. L., Ghodsian, N., Bossé, Y., Thériault, S., Mathieu, P., et al. (2021) · Aging Cell · doi:10.1111/acel.13497 · Cited by 20 · see citing works
Derivation and applications of human hepatocyte-like cells
Li, S., Huang, S., Zhao, Y., Ding, Y.-J., Ma, D., & Ding, Q. (2019) · World Journal of Stem Cells · doi:10.4252/wjsc.v11.i8.535 · Cited by 20 · see citing works
Poorna, M. R., Sudhindran, S., Thampi, M. V., & Mony, U. (2020) · Colloids and Surfaces B Biointerfaces · doi:10.1016/j.colsurfb.2020.111466 · Cited by 17 · see citing works
An Improved Time- and Labor- Efficient Protocol for Mouse Primary Hepatocyte Isolation
Feng, M., DiVall, S. A., & Wu, S. (2021) · Journal of Visualized Experiments · doi:10.3791/61812 · Cited by 16 · see citing works
Ashmore‐Harris, C., Blackford, S. J. I., Grimsdell, B., Kurtys, E., Glatz, M. C., Rashid, S. T., & Fruhwirth, G. O. (2019) · Stem Cell Research · doi:10.1016/j.scr.2019.101599 · Cited by 15 · see citing works
Liver organoids in domestic animals: an expected promise for metabolic studies
Baquerre, C., Montillet, G., & Pain, B. (2021) · Veterinary Research · doi:10.1186/s13567-021-00916-y · Cited by 14 · see citing works
Zhu, W., Li, M., Zou, J., Zhang, D., Fang, M., Sun, Y., Li, C., Tang, M., Wang, Y., Zhou, Q., et al. (2023) · Stem Cell Reports · doi:10.1016/j.stemcr.2023.10.016 · Cited by 13 · see citing works
PCSK9 Inhibition-Based Therapeutic Approaches: An Immunotherapy Perspective
Momtazi‐Borojeni, A. A., Pirro, M., Xu, S., & Sahebkar, A. (2021) · Current Medicinal Chemistry · doi:10.2174/0929867328666211027125245 · Cited by 12 · see citing works
Navigating liver cancer: Precision targeting for enhanced treatment outcomes
Jain, A., Mishra, A. K., Hurkat, P., Shilpi, S., Mody, N., & Jain, S. K. (2025) · Drug Delivery and Translational Research · doi:10.1007/s13346-024-01780-x · Cited by 11 · see citing works
Scalable production of tissue-like vascularised liver organoids from human PSCsPreprint
Harrison, S. P., Siller, R., Tanaka, Y., Xiang, Y., Patterson, B., Kempf, H., Melum, E., Åsrud, K. S., Chollet, M. E., Andersen, E. W., et al. (2020) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2020.12.02.406835 · Cited by 11 · see citing works
APOE4 drives widespread changes to the hepatic proteome and alters metabolic function
Lysaker, C. R., Johnson, C. N., Csikós, V., Franczak, E., Tetlow, A. L., Benson, M. N., Thyfault, J. P., Geiger, P. C., Morris, J. K., & Wilkins, H. (2026) · iScience · doi:10.1016/j.isci.2026.115035 · Cited by 9 · see citing works
Tauran, Y., Poulain, S., Lereau, M., Danoy, M., Shinohara, M., Ségard, B.-D., Kato, S., Kido, T., Miyajima, A., Sakai, Y., et al. (2019) · Molecular Omics · doi:10.1039/c9mo00122k · Cited by 9 · see citing works
Haque, E., Teeli, A. S., Winiarczyk, D., Taguchi, M., Sakuraba, S., Kono, H., Leszczyński, P., Pierzchała, M., & Taniguchi, H. (2022) · Genes · doi:10.3390/genes13030413 · Cited by 9 · see citing works
Derivation and investigation of the first human cell-based model of Beckwith-Wiedemann syndrome
Chang, S., Hur, S. K., Naveh, N. S. S., Thorvaldsen, J. L., French, D. L., Gagne, A. L., Jobaliya, C., Anguera, M. C., Bartolomei, M. S., & Kalish, J. M. (2020) · Epigenetics · doi:10.1080/15592294.2020.1861172 · Cited by 8 · see citing works
Durumutla, H. B., Haller, A. M., Noble, G., Prabakaran, A. D., McFarland, K., Latimer, H., Rajput, A., Akinborewa, O., Namjou‐Khales, B., Hui, D. Y., et al. (2025) · Journal of Clinical Investigation · doi:10.1172/jci190180 · Cited by 7 · see citing works
Cell-intrinsic insulin signaling defects in human iPS cell–derived hepatocytes in type 2 diabetes
Gattu, A. K., Tanzer, M. C., Yaron-Barir, T. M., Johnson, J. L., Jayavelu, A. K., Pan, H., Dreyfuss, J. M., Cantley, L. C., Mann, M., & Kahn, C. R. (2025) · Journal of Clinical Investigation · doi:10.1172/jci183513 · Cited by 7 · see citing works
Weddle, C. J., Blancard, M., Uche, N., Pongpamorn, P., Cejas, R. B., & Burridge, P. W. (2025) · npj Precision Oncology · doi:10.1038/s41698-025-00837-5 · Cited by 6 · see citing works
Zhang, Z., Liu, X., Li, Q., Xie, M., Huang, Y., Jiang, B., Li, X., Huang, Y., & Jian, J. (2023) · Aquaculture · doi:10.1016/j.aquaculture.2023.739912 · Cited by 6 · see citing works
Direct conversion of porcine primary fibroblasts into hepatocyte-like cells
Fráguas-Eggenschwiler, M., Eggenschwiler, R., Söllner, J.-H., Cortnumme, L., Vondran, F. W. R., Cantz, T., Ott, M., & Niemann, H. (2021) · Scientific Reports · doi:10.1038/s41598-021-88727-1 · Cited by 6 · see citing works
Aravalli, R. N., Collins, D. P., Hapke, J. H., Crane, A. T., & Steer, C. J. (2020) · Hepatic Medicine Evidence and Research · doi:10.2147/hmer.s243277 · Cited by 6 · see citing works
Yang, S., Hao, J., Ooka, M., & Xia, M. (2026) · Scientific Reports · doi:10.1038/s41598-025-32539-0 · Cited by 5 · see citing works
Xu, Y., Wang, Y., Qi, R., Li, K., Wang, X., Li, X., & Shi, B. (2024) · International Journal of Medical Sciences · doi:10.7150/ijms.83973 · Cited by 5 · see citing works
Yamashita, H., Fukuda, K., & Hattori, F. (2019) · JMA Journal · doi:10.31662/jmaj.2018-0042 · Cited by 5 · see citing works
Prescott, J. B., Liu, K. J., Lander, A., Pek, N. M. Q., Jha, S. K., Bokelmann, M., Begur, M., Koh, P. W., Yang, H. H., Lim, B., et al. (2025) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2025.02.17.638665 · Cited by 5 · see citing works
Kang, H. K., Kim, D. J., Kim, M. S., Kim, D., Lee, J. Y., Sung, E., Sarsenova, M., Park, S. C., Hong, B. H., & Kang, K. (2022) · 2D Materials · doi:10.1088/2053-1583/ac6ba8 · Cited by 3 · see citing works
Gélinas, R., Lévesque, C., Legault, J. T., Rivard, M.-È., Villeneuve, L. R., Laprise, C., & Rioux, J. D. (2024) · Frontiers in Genetics · doi:10.3389/fgene.2024.1375467 · Cited by 3 · see citing works
Lien, T.-S., Sun, D., & Chang, H. (2024) · Cells · doi:10.3390/cells13211778 · Cited by 2 · see citing works
Huo, Z., Tu, J. F., Yang, W., Huang, M.-F., Wang, R., Chu, C.-W., Xu, A., Yu, Y. T., Tavakol, T. N., Kizilbash, M., et al. (2026) · Journal of Biological Chemistry · doi:10.1016/j.jbc.2026.111435 · Cited by 2 · see citing works
Metabolic impact of extrahepatic PCSK9 modulation: Extrahepatic PCSK9 modulation
Dalt, L. D., & Bonacina, F. (2022) · European Atherosclerosis Journal · doi:10.56095/eaj.v1i2.13 · Cited by 2 · see citing works
Júnior, W. F. D. S., Costa, K. M. D. O., Oliveira, H. M. D. C., Antunes, M. M., Mafra, K., Nakagaki, B. N., da Silva, P. S. C., Megale, J. D., de Sales, S. C., Caixeta, D. C., et al. (2025) · Journal of Lipid Research · doi:10.1016/j.jlr.2025.100744 · Cited by 3 · see citing works
Tadokoro, S., Tokuyama-Toda, R., Tatehara, S., Ide, S., Umeki, H., Miyoshi, K., Noma, T., & Satomura, K. (2021) · Cells · doi:10.3390/cells10112827 · Cited by 2 · see citing works
Downstream bioprocessing of human pluripotent stem cell‐derived therapeutics
Sart, S., Liu, C., Zeng, E. Z., Xu, C., & Li, Y. (2021) · Engineering in Life Sciences · doi:10.1002/elsc.202100042 · Cited by 2 · see citing works
Hypoimmunogenic iPSC-derived hepatic organoids featuring a functional vascular network
Caroli, A., Varinelli, M., Vigani, L., Vallerga, A., Rizzo, P., Rottoli, D., Conti, S., Craparotta, I., Fumagalli, S., Remuzzi, G., et al. (2026) · Scientific Reports · doi:10.1038/s41598-026-58977-y · Cited by 1 · see citing works
Yamashita, H., & Hattori, F. (2023) · Journal of Visualized Experiments · doi:10.3791/65777 · Cited by 1 · see citing works
Stem cell modeling of lipid genetics
Musunuru, K. (2018) · Current Opinion in Lipidology · doi:10.1097/mol.0000000000000490 · Cited by 1 · see citing works
Self-citationŁukasiak, M., Gatti, G., Chung, S., Kiloh, G., Robinson, C., Kasioulis, I., Gil, C., Panman, L., & Nikolaou, N. (2024) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2024.11.18.624145 · Cited by 1 · see citing works
[Leukocyte count of puerperal sows].
Mäde, D., & Wujanz, G. (1996) · PubMed
Engenharia tecidual hepática utilizando células tronco pluripotentes induzidas
Guimarães, E. D. S. G. (2019) · doi:10.11606/t.41.2019.tde-27082019-093823
Generation of a human iPSC line CIBi010-A with a reporter for ASGR1 using CRISPR/Cas9
Fu, J., Jiang, L., Yu, B., Liu, Y., Wei, R., Hu, Y., Ho, W.-I., Yang, B., Chu, M., Tse, H., et al. (2022) · Stem Cell Research · doi:10.1016/j.scr.2022.102800
Serum GP73, Nrf2, CHE in early pregnancy-related liver injury detection via RCS model
Huang, D., Hu, Y.-J., Dong, B., Zheng, Z., Wang, H., Liao, T., Wang, W., Zheng, X., Xu, Z., Xia, S., et al. (2025) · Biomarkers in Medicine · doi:10.1080/17520363.2025.2516998
A novel experimental in vitro human definitive endoderm cell-derived model of Wilson disease
Berestovoy, M. A., Starodubova, V. D., Karpukhina, E. V., Baranova, A. S., & Shokhina, A. G. (2025) · Genes and Cells · doi:10.17816/gc677378
Liu, S., Cao, X., Wu, H., Zhang, S., Zhang, X., Chen, S., Shan, H., Gu, W., Zhou, Y., & Duan, Y. (2026) · Stem Cells · doi:10.1093/stmcls/sxag016
Bellesis, A., Li, X., Moore-Frederick, D., Xu, D., Delbridge, K., Ma, J., Vaccaro, G., Edward, B. A. A., Kellogg, M., Creeger, Y., et al. (2026) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.64898/2026.06.05.730479
Sun, S., Chen, H., Hu, Y., Lin, Z., Ye, H., Zhang, Q., Zhao, J., Liu, X., & Liu, Z. (2026) · Ecotoxicology and Environmental Safety · doi:10.1016/j.ecoenv.2026.120927
- Authors
- Peters, D. T., Henderson, C. A., Warren, C. R., Friesen, M., Xia, F., Becker, C. E., Musunuru, K., & Cowan, C. A.
- Affiliations
- Harvard University (US)
- Brigham and Women's Hospital (US)
- Corresponding author
- Cowan, Chad A.
- Venue
- Development
- Publisher
- The Company of Biologists
- Published
- 2016-05-01
- Volume
- 143
- Issue
- 9
- Pages
- 1475-1481
- ISSN
- 0950-1991 (ISSN-L)
- 1477-9129
- PMC
- PMC4909860
- OpenAlex
- W2345599021
- Cited by
- 68
- References
- 23
- Citations by year
- 2026: 7 · 2025: 8 · 2024: 6 · 2023: 4 · 2022: 6 · 2021: 8 · 2020: 9 · 2019: 12 · 2018: 3 · 2017: 3 · 2016: 1
- FWCI
- 3.29
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Development (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- HighWire Press Open Archive (submittedVersion)
- Funders
- Harvard Stem Cell Institute
- National Heart, Lung, and Blood Institute (R21HL120781, U01HL100408, U01HL107440)
- National Institute of Diabetes and Digestive and Kidney Diseases (R01DK097768, P30DK040561)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Pluripotent Stem Cells Research
- Health Sciences › Medicine › Genetics › Mesenchymal stem cell research
- Health Sciences › Medicine › Hepatology › Liver physiology and pathology
- Keywords
- ASGR1, hepatocyte-like cells, human pluripotent stem cells, hepatocyte differentiation, cell surface markers, cell sorting, albumin secretion, urea secretion, hepatocyte heterogeneity
- MeSH terms
- Albumins, Cell Differentiation, Cell Line, Cell Membrane, Cytochromes, Flow Cytometry, Humans, Urea, Hepatocytes, Asialoglycoprotein Receptor, Embryonic Stem Cells, Induced Pluripotent Stem Cells
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