Highly efficient generation of human hepatocyte-like cells from induced pluripotent stem cells
Open accessAbstract
There exists a worldwide shortage of donor livers available for orthotropic liver transplantation and hepatocyte transplantation therapies. In addition to their therapeutic potential, primary human hepatocytes facilitate the study of molecular and genetic aspects of human hepatic disease and development and provide a platform for drug toxicity screens and identification of novel pharmaceuticals with potential to treat a wide array of metabolic diseases. The demand for human hepatocytes, therefore, heavily outweighs their availability. As an alternative to using donor livers as a source of primary hepatocytes, we explored the possibility of generating patient-specific human hepatocytes from induced pluripotent stem (iPS) cells. Conclusion: We demonstrate that mouse iPS cells retain full potential for fetal liver development and describe a procedure that facilitates the efficient generation of highly differentiated human hepatocyte-like cells from iPS cells that display key liver functions and can integrate into the hepatic parenchyma in vivo . (Hepatology 2010.)
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HNF4A+ liver progenitors were typically formed on later days (by day 10-14) in other differentiation protocols.
“whereas HNF4A+ liver progenitors were typically formed on later days (by day 10–14) in other differentiation protocols (Carpentier et al., 2016; Hay et al., 2008; Si-Tayeb et al., 2010; Zhao et al., 2013).”
From A Roadmap for Human Liver Differentiation from Pluripotent Stem Cells (2018) · cites this paper as Si-Tayeb et al., 2010
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Cites
Takahashi, K., & Yamanaka, S. (2006) · Cell · doi:10.1016/j.cell.2006.07.024 · Cited by 26,673 · see citing works
Disease-Specific Induced Pluripotent Stem Cells.
Park, I.-H., Arora, N., Huo, H., Maherali, N., Ahfeldt, T., Shimamura, A., Lensch, M. W., Cowan, C., Hochedlinger, K., & Daley, G. Q. (2008) · Cell · doi:10.1016/j.cell.2008.07.041 · Cited by 2,196 · see citing works
Flagged first, then by citations
Research works printed but not linked: 33
- [1]Lowry WE Richter L Yachechko R Generation of human induced pluripotent stem cells from dermal fibroblasts Proc Natl Acad Sci U S A 2008 105 2883 2888 18287077 PMC2268554
- [2]Park IH Zhao R West JA Reprogramming of human somatic cells to pluripotency with defined factors Nature 2008 451 141 146 18157115
- [3]Takahashi K Tanabe K Ohnuki M Induction of pluripotent stem cells from adult human fibroblasts by defined factors Cell 2007 131 861 872 18035408
- [5]Yu J Vodyanik MA Smuga-Otto K Induced pluripotent stem cell lines derived from human somatic cells Science 2007 318 1917 1920 18029452
- [7]Burlina AB Hepatocyte transplantation for inborn errors of metabolism J Inherit Metab Dis 2004 27 373 383 15190195
- [8]Fisher RA Strom SC Human hepatocyte transplantation: worldwide results Transplantation 2006 82 441 449 16926585
- [9]Grompe M Principles of therapeutic liver repopulation J Inherit Metab Dis 2006 29 421 425 16763912
- [10]Gupta S Chowdhury JR Therapeutic potential of hepatocyte transplantation Semin Cell Dev Biol 2002 13 439 446 12468245
- [11]Nagy A Rossant J Nagy R Derivation of completely cell culture-derived mice from early passage embryonic stem cells Proc Natl Acad Sci USA 1993 90 8424 8428 8378314 PMC47369
- [12]Agarwal S Holton KL Lanza R Efficient differentiation of functional hepatocytes from human embryonic stem cells Stem Cells 2008 26 1117 1127 18292207
- [13]Cai J Zhao Y Liu Y Directed differentiation of human embryonic stem cells into functional hepatic cells Hepatology 2007 45 1229 1239 17464996
- [14]Shiraki N Umeda K Sakashita N Differentiation of mouse and human embryonic stem cells into hepatic lineages Genes Cells 2008 13 731 746 18513331
- [15]D'Amour KA Agulnick AD Eliazer S Efficient differentiation of human embryonic stem cells to definitive endoderm Nat Biotechnol 2005 23 1534 1541 16258519
- [16]McLean AB D'Amour KA Jones KL Activin a efficiently specifies definitive endoderm from human embryonic stem cells only when phosphatidylinositol 3-kinase signaling is suppressed Stem Cells 2007 25 29 38 17204604
- [17]Jung J Zheng M Goldfarb M Initiation of mammalian liver development from endoderm by fibroblast growth factors Science 1999 284 1998 2003 10373120
- [18]Rossi JM Dunn NR Hogan BL Distinct mesodermal signals, including BMPs from the septum transversum mesenchyme, are required in combination for hepatogenesis from the endoderm Genes Dev 2001 15 1998 2009 11485993 PMC312750
- [19]Watt AJ Zhao R Li J Development of the mammalian liver and ventral pancreas is dependent on GATA4 BMC Dev Biol 2007 7 37 17451603 PMC1877807
- [20]Zhao R Watt AJ Li J GATA6 is essential for embryonic development of the liver but dispensable for early heart formation Mol Cell Biol 2005 25 2622 2631 15767668 PMC1061656
- [21]Duncan SA Manova K Chen WS Expression of transcription factor HNF-4 in the extraembryonic endoderm, gut, and nephrogenic tissue of the developing mouse embryo: HNF-4 is a marker for primary endoderm in the implanting blastocyst Proc Natl Acad Sci USA 1994 91 7598 7602 8052626 PMC44449
- [22]Kamiya A Kinoshita T Ito Y Fetal liver development requires a paracrine action of oncostatin M through the gp130 signal transducer Embo J 1999 18 2127 2136 10205167 PMC1171297
- [23]Ge X Yamamoto S Tsutsumi S Interpreting expression profiles of cancers by genome-wide survey of breadth of expression in normal tissues Genomics 2005 86 127 141 15950434
- [24]Strain AJ Isolated hepatocytes: use in experimental and clinical hepatology Gut 1994 35 433 436 8174975 PMC1374784
- [25]Baharvand H Hashemi SM Kazemi Ashtiani S Differentiation of human embryonic stem cells into hepatocytes in 2D and 3D culture systems in vitro Int J Dev Biol 2006 50 645 652 16892178
- [26]Baharvand H Hashemi SM Shahsavani M Differentiation of human embryonic stem cells into functional hepatocyte-like cells in a serum-free adherent culture condition Differentiation 2008 76 465 477 18177418
- [27]Chiao E Elazar M Xing Y Isolation and transcriptional profiling of purified hepatic cells derived from human embryonic stem cells Stem Cells 2008 26 2032 2041 18535157
- [28]Cho CH Parashurama N Park EY Homogeneous differentiation of hepatocyte-like cells from embryonic stem cells: applications for the treatment of liver failure FASEB J 2008 22 898 909 17942827
- [29]Hay DC Fletcher J Payne C Highly efficient differentiation of hESCs to functional hepatic endoderm requires ActivinA and Wnt3a signaling Proc Natl Acad Sci U S A 2008 105 12301 12306 18719101 PMC2518825
- [30]Hay DC Zhao D Fletcher J Efficient differentiation of hepatocytes from human embryonic stem cells exhibiting markers recapitulating liver development in vivo Stem Cells 2008 26 894 902 18238852
- [31]Muraca M Gerunda G Neri D Hepatocyte transplantation as a treatment for glycogen storage disease type 1a Lancet 2002 359 317 318 11830200
- [32]Mikkelsen TS Hanna J Zhang X Dissecting direct reprogramming through integrative genomic analysis Nature 2008 454 49 55 18509334 PMC2754827
- [33]Waring JF Ciurlionis R Jolly RA Isolated human hepatocytes in culture display markedly different gene expression patterns depending on attachment status Toxicol In Vitro 2003 17 693 701 14599465
- [34]Madan A Graham RA Carroll KM Effects of prototypical microsomal enzyme inducers on cytochrome P450 expression in cultured human hepatocytes Drug Metab Dispos 2003 31 421 431 12642468
- [35]Khetani SR Bhatia SN Microscale culture of human liver cells for drug development Nat Biotechnol 2008 26 120 126 18026090
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BackgroundResultInfluentialHNF4A is essential for specification of hepatic progenitors from human pluripotent stem cells
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Yan, Y., Bejoy, J., Xia, J., Griffin, K., Guan, J., & Li, Y. (2019) · Scientific Reports · doi:10.1038/s41598-018-37686-1 · Cited by 27 · see citing works
Yang, G., Si-Tayeb, K., Corbineau, S., Vernet, R., Gayon, R., Dianat, N., Martinet, C., Clay, D., Goulinet-Mainot, S., Tachdjian, G., et al. (2013) · BMC Biology · doi:10.1186/1741-7007-11-86 · Cited by 26 · see citing works
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Hay, D. C. (2010) · Gastroenterology · doi:10.1053/j.gastro.2010.07.013 · Cited by 9 · see citing works
TBX3 advances the developmental chromatin landscape toward the hepatic fate
Stephan, T. L., Cullum, R., Drissler, S., Thomas, A., Fuglerud, B. M., Clement-Ranney, M., Lotto, J., Krentz, N. A. J., Duncan, S. A., & Hoodless, P. A. (2025) · Developmental Cell · doi:10.1016/j.devcel.2025.05.014 · Cited by 1 · see citing works
Self-citationCryopreservation of Hepatocyte Organoid-Derived Cells Generated from Human iPS Cells
Nagai, W., Inui, J., Ueyama-Toba, Y., Asano, R., & Mizuguchi, H. (2025) · Biological and Pharmaceutical Bulletin · doi:10.1248/bpb.b25-00276 · Cited by 1 · see citing works
Ghanem, S. S., Al-Barazenji, T., Badi, R., & Abdelalim, E. M. (2026) · Molecular Biology Reports · doi:10.1007/s11033-026-12480-9
- Authors
- Si-Tayeb, K., Noto, F. K., Nagaoka, M., Li, J., Battle, M. A., Duris, C., North, P. E., Dalton, S., & Duncan, S. A.
- Affiliations
- Medical College of Wisconsin (US)
- University of Georgia (US)
- Corresponding author
- Duncan, Stephen A.
- Venue
- Hepatology
- Published
- 2010-01
- Volume
- 51
- Issue
- 1
- Pages
- 297-305
- ISSN
- 0270-9139 (ISSN-L)
- PMC
- PMC2946078
- OpenAlex
- W2020755558
- Cited by
- 1,282
- References
- 2
- Citations by year
- 2026: 33 · 2025: 49 · 2024: 39 · 2023: 57 · 2022: 80 · 2021: 86 · 2020: 96 · 2019: 94 · 2018: 97 · 2017: 89 · 2016: 105 · 2015: 89 · 2014: 91 · 2013: 86 · 2012: 77
- FWCI
- 25.53
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Hepatology (publishedVersion)
- PubMed (publishedVersion)
- PubMed Central (acceptedVersion)
- Funders
- National Heart, Lung, and Blood Institute (P01HL089471)
- National Institute of General Medical Sciences (P01GM085354)
- National Institute of Diabetes and Digestive and Kidney Diseases (R01DK055743, RC1DK087377)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Pluripotent Stem Cells Research
- Health Sciences › Medicine › Genetics › Mesenchymal stem cell research
- Health Sciences › Medicine › Hepatology › Liver physiology and pathology
- Keywords
- hepatocyte-like cells, induced pluripotent stem cells, fetal liver development, hepatocyte transplantation, liver transplantation, hepatocyte differentiation, drug toxicity testing, metabolic disorders
- MeSH terms
- Animals, Cell Differentiation, Humans, Hepatocytes, Mice, Induced Pluripotent Stem Cells
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