The DOI matches its Crossref record; 12 of the 12 references it cites were found in a registry or an index.
Integrated analysis of single-cell embryo data yields a unified transcriptome signature for the human preimplantation epiblast
Open accessAbstract
Single-cell profiling techniques create opportunities to delineate cell fate progression in mammalian development. Recent studies provide transcriptome data from human preimplantation embryos, in total comprising nearly 2000 individual cells. Interpretation of these data is confounded by biological factors such as variable embryo staging and cell-type ambiguity, as well as technical challenges in the collective analysis of datasets produced with different sample preparation and sequencing protocols. Here we address these issues to assemble a complete gene expression time course spanning human preimplantation embryogenesis. We identify key transcriptional features over developmental time and elucidate lineage-specific regulatory networks. We resolve post hoc cell-type assignment in the blastocyst, and define robust transcriptional prototypes that capture epiblast and primitive endoderm lineages. Examination of human pluripotent stem cell transcriptomes in this framework identifies culture conditions that sustain a naïve state pertaining to the inner cell mass. Our approach thus clarifies understanding both of lineage segregation in the early human embryo and of in vitro stem cell identity, and provides an analytical resource for comparative molecular embryology.
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Cites
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Self-citationSingle-Cell RNA-Seq Reveals Dynamic, Random Monoallelic Gene Expression in Mammalian Cells
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Cited by
Development of the human placenta
Turco, M. Y., & Moffett, A. (2019) · Development · doi:10.1242/dev.163428 · Cited by 698
Chemical reprogramming of human somatic cells to pluripotent stem cells.
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Single-cell analysis of human embryos reveals diverse patterns of aneuploidy and mosaicism
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Metadata
- Authors
- Stirparo, G. G., Boroviak, T., Guo, G., Nichols, J., Smith, A., & Bertone, P.
- Venue
- Development
- Publisher
- The Company of Biologists
- Published
- 2018-01-01
- Volume
- 145
- Issue
- 3
- ISSN
- 0950-1991 (ISSN-L)
- 1477-9129
- OpenAlex
- W2773077407
- Cited by
- 206
- References
- 12
- FWCI
- 8.71
- Type
- journal article
- Language
- en
- Open access
- hybrid
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