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Cited by 21Developmental Biology and Gene RegulationNeurogenesis and neuroplasticity mechanismsGene Regulatory Network Analysis

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Unravelling differential Hes1 dynamics during axis elongation of mouse embryos through single-cell tracking

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el Azhar, Yasmine, Schulthess, Pascal, van Oostrom, Marek J., Weterings, Sonja D. C., Meijer, Wilke H. M., Tsuchida-Straeten, Nobuko, Thomas, Wouter M., Bauer, Marianne, Sonnen, Katharina F.·2024·Development
DOI 10.1242/dev.202936

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Abstract

The intricate dynamics of Hes expression across diverse cell types in the developing vertebrate embryonic tail have remained elusive. To address this, we have developed an endogenously tagged Hes1-Achilles mouse line, enabling precise quantification of dynamics at the single-cell resolution across various tissues. Our findings reveal striking disparities in Hes1 dynamics between presomitic mesoderm (PSM) and preneural tube (pre-NT) cells. While pre-NT cells display variable, low-amplitude oscillations, PSM cells exhibit synchronized, high-amplitude oscillations. Upon the induction of differentiation, the oscillation amplitude increases in pre-NT cells. Additionally, our study of Notch inhibition on Hes1 oscillations unveils distinct responses in PSM and pre-NT cells, corresponding to differential Notch ligand expression dynamics. These findings suggest the involvement of separate mechanisms driving Hes1 oscillations. Thus, Hes1 demonstrates dynamic behaviour across adjacent tissues of the embryonic tail, yet the varying oscillation parameters imply differences in the information that can be transmitted by these dynamics.

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Metadata

Authors
el Azhar, Y., Schulthess, P., van Oostrom, M. J., Weterings, S. D. C., Meijer, W. H. M., Tsuchida-Straeten, N., Thomas, W. M., Bauer, M., & Sonnen, K. F.
Affiliations
  • Royal Netherlands Academy of Arts and Sciences (NL)
  • European Molecular Biology Laboratory (DE)
  • Delft University of Technology (NL)
ORCID
Venue
Development
Publisher
The Company of Biologists
Published
2024-09-15
Volume
151
Issue
18
ISSN
  • 0950-1991 (ISSN-L)
  • 1477-9129
DOI
10.1242/dev.202936
OpenAlex
W4402769554
Cited by
21
References
3
Citations by year
2026: 8 · 2025: 10 · 2024: 3
FWCI
2.83
Type
journal article
Language
en
Open access
Licence
Locations
  • Development (publishedVersion, cc-by)
  • bioRxiv (Cold Spring Harbor Laboratory) (acceptedVersion, cc-by-nc-nd)
  • PubMed (publishedVersion)
  • KNAW Research Portal (The Royal Netherlands Academy of Arts and Sciences) (publishedVersion, other-oa)
  • Utrecht University Repository (Utrecht University) (submittedVersion, other-oa)
Funders
  • KWF Kankerbestrijding (13661)
  • European Research Council (850554)
  • National Science Foundation (PHY-1748958)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Developmental Biology and Gene Regulation
  • Life Sciences › Neuroscience › Developmental Neuroscience › Neurogenesis and neuroplasticity mechanisms
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gene Regulatory Network Analysis
Keywords
Hes1, Notch signaling, gene expression oscillations, presomitic mesoderm, axial elongation, cell fate specification, single-cell tracking
MeSH terms
Transcription Factor HES-1, Animals, Cell Differentiation, Embryo, Mammalian, Mesoderm, Tail, Gene Expression Regulation, Developmental, Somites, Body Patterning, Embryonic Development, Mice, Receptors, Notch, Single-Cell Analysis

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