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Cited by 422

The DOI matches its Crossref record; 43 of the 43 references it cites were found in a registry or an index.

Claudin-2, a component of the tight junction, forms a paracellular water channel

Rosenthal, Rita, Milatz, Susanne, Krug, Susanne M., Oelrich, Beibei, Schulzke, Jörg-Dieter, Amasheh, Salah, Günzel, Dorothee, Fromm, Michael·2010·Journal of Cell Science
DOI 10.1242/jcs.060665
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Abstract

Whether or not significant amounts of water pass the tight junction (TJ) of leaky epithelia is still unresolved, because it is difficult to separate transcellular water flux from TJ-controlled paracellular water flux. Using an approach without differentiating technically between the transcellular and paracellular route, we measured transepithelial water flux with and without selective molecular perturbation of the TJ to unequivocally attribute changes to the paracellular pathway. To this end, MDCK C7 cells were stably transfected with either claudin-2 or claudin-10b, two paracellular cation-channel-forming TJ proteins that are not endogenously expressed in this cell line. Claudin-2 is typical of leaky, water-transporting epithelia, such as the kidney proximal tubule, whereas claudin-10b is present in numerous epithelia, including water-impermeable segments of the loop of Henle. Neither transfection altered the expression of endogenous claudins or aquaporins. Water flux was induced by an osmotic gradient, a Na+ gradient or both. Under all conditions, water flux in claudin-2-transfected cells was elevated compared with vector controls, indicating claudin-2-mediated paracellular water permeability. Na+-driven water transport in the absence of an osmotic gradient indicates a single-file mechanism. By contrast, claudin-10b transfection did not alter water flux. We conclude that claudin-2, but not claudin-10b, forms a paracellular water channel and thus mediates paracellular water transport in leaky epithelia.

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Metadata

Authors
Rosenthal, R., Milatz, S., Krug, S. M., Oelrich, B., Schulzke, J.-D., Amasheh, S., Günzel, D., & Fromm, M.
Venue
Journal of Cell Science
Publisher
The Company of Biologists
Published
2010-06-01
Volume
123
Issue
11
Pages
1913-1921
ISSN
  • 0021-9533 (ISSN-L)
  • 1477-9137
DOI
10.1242/jcs.060665
OpenAlex
W1998461769
Cited by
422
References
43
FWCI
9.33
Type
journal article
Language
en
Open access
closed

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CoChat. (2026). Citation check: Claudin-2, a component of the tight junction, forms a paracellular water channel. https://app.cochat.ai/papers/10.1242/jcs.060665
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Rosenthal, R., Milatz, S., Krug, S. M., Oelrich, B., Schulzke, J.-D., Amasheh, S., Günzel, D., & Fromm, M. (2010). Claudin-2, a component of the tight junction, forms a paracellular water channel. Journal of Cell Science. https://doi.org/10.1242/jcs.060665

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