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An RNA-zipcode-independent mechanism that localizes Dia1 mRNA to the perinuclear ER through interactions between Dia1 nascent peptide and Rho–GTP

Liao, Guoning, Ma, Xinghong, Liu, Gang·2011·Journal of Cell Science
DOI 10.1242/jcs.072421
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Abstract

Signal-peptide-mediated ER localization of mRNAs encoding for membrane and secreted proteins, and RNA-zipcode-mediated intracellular targeting of mRNAs encoding for cytosolic proteins are two well-known mechanisms for mRNA localization. Here, we report a previously unidentified mechanism by which mRNA encoding for Dia1, a cytosolic protein without the signal peptide, is localized to the perinuclear ER in an RNA-zipcode-independent manner in fibroblasts. Dia1 mRNA localization is also independent of the actin and microtubule cytoskeleton but requires translation and the association of Dia1 nascent peptide with the ribosome–mRNA complex. Sequence mapping suggests that interactions of the GTPase binding domain of Dia1 peptide with active Rho are important for Dia1 mRNA localization. This mechanism can override the β-actin RNA zipcode and redirect β-actin mRNA to the perinuclear region, providing a new way to manipulate intracellular mRNA localization.

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Metadata

Authors
Liao, G., Ma, X., & Liu, G.
Affiliations
Center for Cell Biology and Cancer Research, Albany Medical College, Albany, NY 12208, USA
Venue
Journal of Cell Science
Publisher
The Company of Biologists
Published
2011-02-15
Volume
124
Issue
4
Pages
589-599
ISSN
  • 1477-9137
  • 0021-9533
DOI
10.1242/jcs.072421
Cited by
25
References
0
Language
en

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