Cited by 49MicroRNA in disease regulationMitochondrial Function and PathologyExtracellular vesicles in disease

The Mitochondria-Associated ER Membranes Are Novel Subcellular Locations Enriched for Inflammatory-Responsive MicroRNAs

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Wang, Wang-Xia, Prajapati, Paresh, Nelson, Peter T., Springer, Joe E.·2020·Molecular Neurobiology

Abstract

The mitochondria-associated endoplasmic reticulum (ER) membranes (MAMs) are specific ER domains that contact the mitochondria and function to facilitate communication between ER and mitochondria. Disruption of contact between the mitochondria and ER is associated with a variety of pathophysiological conditions including neurodegenerative diseases. Considering the many cellular functions of MAMs, we hypothesized that MAMs play an important role in regulating microRNA (miRNA) activity linked to its unique location between mitochondria and ER. Here we present new findings from human and rat brains indicating that the MAMs are subcellular sites enriched for specific miRNAs. We employed subcellular fractionation and TaqMan® RT-qPCR miRNA analysis to quantify miRNA levels in subcellular fractions isolated from male rat brains and six human brain samples. We found that MAMs contain a substantial number of miRNAs and the profile differs significantly from that of cytosolic, mitochondria, or ER. Interestingly, MAMs are particularly enriched in inflammatory-responsive miRNAs, including miR-146a, miR-142-3p, and miR-142-5p in both human and rat brains; miR-223 MAM enrichment was observed only in human brain samples. Further, mitochondrial uncoupling or traumatic brain injury in male rats resulted in the alteration of inflammatory miRNA enrichment in the isolated subcellular fractions. These observations demonstrate that miRNAs are distributed differentially in organelles and may re-distribute between organelles and the cytosol in response to cellular stress and metabolic demands.

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Authors
Wang, W.-X., Prajapati, P., Nelson, P. T., & Springer, J. E.
Affiliations
University of Kentucky (US)
Corresponding author
Wang, Wang-Xia
Venue
Molecular Neurobiology
Published
2020-05-25
Volume
57
Issue
7
Pages
2996-3013
ISSN
  • 0893-7648 (ISSN-L)
  • 1559-1182
OpenAlex
W3032509796
Cited by
49
References
83
Citations by year
2026: 3 · 2025: 6 · 2024: 19 · 2023: 7 · 2022: 6 · 2021: 6 · 2020: 2
FWCI
1.68
Type
journal article
Language
en
Open access
Locations
  • Molecular Neurobiology (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
Funders
Kentucky Spinal Cord and Head Injury Research Trust (KSCHIRT 15-12A, KSCHIRT 18 8A)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › MicroRNA in disease regulation
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Mitochondrial Function and Pathology
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Extracellular vesicles in disease
Keywords
mitochondria-associated endoplasmic reticulum membranes, neuroinflammation, miR-146a, miR-142-3p, miR-142-5p, miR-223, mitochondrial uncoupling, traumatic brain injury, ER-mitochondria communication, brain
MeSH terms
Aged, Aged, 80 and over, Animals, Brain, Cytosol, Dementia, Endoplasmic Reticulum, Female, Humans, Inflammation, Intracellular Membranes, Male, Mitochondria, Subcellular Fractions, Rats, Sprague-Dawley, MicroRNAs, Rats, Cognitive Dysfunction

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