The DOI matches its Crossref record; 34 of the 34 references it cites were found in a registry or an index.
Sonic hedgehog increases the commitment of pluripotent mesenchymal cells into the osteoblastic lineage and abolishes adipocytic differentiation
Abstract
The proteins of the hedgehog (Hh) family regulate various aspects of development. Recently, members of this family have been shown to regulate skeletal formation in vertebrates and to control both chondrocyte and osteoblast differentiation. In the present study, we analyzed the effect of Sonic hedgehog (Shh) on the osteoblastic and adipocytic commitment/differentiation. Recombinant N-terminal Shh (N-Shh) significantly increased the percentage of both the pluripotent mesenchymal cell lines C3H10T1/2 and ST2 and calvaria cells responding to bone morphogenetic protein 2 (BMP-2), in terms of osteoblast commitment as assessed by measuring alkaline phosphatase (ALP) activity. This synergistic effect was mediated, at least partly, through the positive modulation of the transcriptional output of BMPs via Smad signaling. Furthermore, N-Shh was found to abolish adipocytic differentiation of C3H10T1/2 cells both in the presence or absence of BMP-2. A short treatment with N-Shh was sufficient to dramatically reduce the levels of the adipocytic-related transcription factors C/EBPα and PPARγ in both C3H10T1/2 and calvaria cell cultures. Given the inverse relationship between marrow adipocytes and osteoblasts with aging, agonists of the Hh signaling pathway might constitute potential drugs for preventing and/or treating osteopenic disorders.
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Cites
TGF-β signalling from cell membrane to nucleus through SMAD proteins
Heldin, C.-H., Miyazono, K., & ten Dijke, P. (1997) · Nature · doi:10.1038/37284 · Cited by 3,696
Cyclopia and defective axial patterning in mice lacking Sonic hedgehog gene function
Chiang, C., Litingtung, Y., Lee, E., Young, K. E., Corden, J. L., Westphal, H., & Beachy, P. A. (1996) · Nature · doi:10.1038/383407a0 · Cited by 3,062
PPARγ Is Required for the Differentiation of Adipose Tissue In Vivo and In Vitro
Rosen, E. D., Sarraf, P., Troy, A. E., Bradwin, G., Moore, K., Milstone, D. S., Spiegelman, B. M., & Mortensen, R. M. (1999) · Molecular Cell · doi:10.1016/s1097-2765(00)80211-7 · Cited by 2,079
Regulation of Rate of Cartilage Differentiation by Indian Hedgehog and PTH-Related Protein
Vortkamp, A., Lee, K., Lanske, B., Segre, G. V., Kronenberg, H. M., & Tabin, C. J. (1996) · Science · doi:10.1126/science.273.5275.613 · Cited by 1,994
NEW EMBO MEMBERS REVIEW: Transcriptional control by the TGF-beta/Smad signaling system
Massague, J. (2000) · The EMBO Journal · doi:10.1093/emboj/19.8.1745 · Cited by 1,966
Transcriptional regulation of adipogenesis
Rosen, E. D., Walkey, C. J., Puigserver, P., & Spiegelman, B. M. (2000) · Genes & Development · doi:10.1101/gad.14.11.1293 · Cited by 1,898
St-Jacques, B., Hammerschmidt, M., & McMahon, A. P. (1999) · Genes & Development · doi:10.1101/gad.13.16.2072 · Cited by 1,735
The Spemann Organizer Signal noggin Binds and Inactivates Bone Morphogenetic Protein 4
Zimmerman, L. B., De Jesús-Escobar, J. M., & Harland, R. M. (1996) · Cell · doi:10.1016/s0092-8674(00)80133-6 · Cited by 1,633
The tumour-suppressor gene patched encodes a candidate receptor for Sonic hedgehog
Stone, D. M., Hynes, M., Armanini, M., Swanson, T. A., Gu, Q., Johnson, R. L., Scott, M. P., Pennica, D., Goddard, A., Phillips, H., et al. (1996) · Nature · doi:10.1038/384129a0 · Cited by 1,106
Transcriptional Activators of TGF-β Responses: Smads
Derynck, R., Zhang, Y., & Feng, X.-H. (1998) · Cell · doi:10.1016/s0092-8674(00)81696-7 · Cited by 1,029
Cited by
Adipocyte differentiation from the inside out
Rosen, E. D., & MacDougald, O. A. (2006) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm2066 · Cited by 2,607
LDL Receptor-Related Protein 5 (LRP5) Affects Bone Accrual and Eye Development
Gong, Y., Slee, R. B., Fukai, N., Rawadi, G., Roman-Roman, S., Reginato, A. M., Wang, H., Cundy, T., Glorieux, F. H., Lev, D., et al. (2001) · Cell · doi:10.1016/s0092-8674(01)00571-2 · Cited by 2,235
Self-citationFate decision of mesenchymal stem cells: adipocytes or osteoblasts?
Chen, Q., Shou, P., Zheng, C., Jiang, M., Cao, G., Yang, Q., Cao, J., Xie, N., Velletri, T., Zhang, X., et al. (2016) · Cell Death & Differentiation · doi:10.1038/cdd.2015.168 · Cited by 1,156
1 Developmental roles and clinical significance of Hedgehog signaling
McMahon, A. P., Ingham, P. W., & Tabin, C. J. (2003) · Current Topics in Developmental Biology · doi:10.1016/s0070-2153(03)53002-2 · Cited by 772
Sequential roles of Hedgehog and Wnt signaling in osteoblast development
Hu, H., Hilton, M. J., Tu, X., Yu, K., Ornitz, D. M., & Long, F. (2005) · Development · doi:10.1242/dev.01564 · Cited by 682
Metadata
- Authors
- Spinella-Jaegle, S., Rawadi, G., Kawai, S., Gallea, S., Faucheu, C., Mollat, P., Courtois, B., Bergaud, B., Ramez, V., Blanchet, A. M., Adelmant, G., Baron, R., & Roman-Roman, S.
- Venue
- Journal of Cell Science
- Publisher
- The Company of Biologists
- Published
- 2001-06-01
- Volume
- 114
- Issue
- 11
- Pages
- 2085-2094
- ISSN
- 0021-9533 (ISSN-L)
- 1477-9137
- OpenAlex
- W1944656442
- Cited by
- 267
- References
- 34
- FWCI
- 3.37
- Type
- journal article
- Language
- en
- Open access
- closed
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- Spinella-Jaegle, S., Rawadi, G., Kawai, S., Gallea, S., Faucheu, C., Mollat, P., Courtois, B., Bergaud, B., Ramez, V., Blanchet, A. M., Adelmant, G., Baron, R., & Roman-Roman, S. (2001). Sonic hedgehog increases the commitment of pluripotent mesenchymal cells into the osteoblastic lineage and abolishes adipocytic differentiation. Journal of Cell Science. https://doi.org/10.1242/jcs.114.11.2085
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
