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Cited by 97Cellular Mechanics and InteractionsCell Adhesion Molecules ResearchForce Microscopy Techniques and Applications

E-cadherin-mediated force transduction signals regulate global cell mechanics

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Muhamed, Ismaeel, Wu, Jun, Sehgal, Poonam, Kong, Xinyu, Tajik, Arash, Wang, Ning, Leckband, Deborah E.·2016·Journal of Cell Science
DOI 10.1242/jcs.185447PMC PMC4893802
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Abstract

This report elucidates an E-cadherin-based force-transduction pathway that triggers changes in cell mechanics through a mechanism requiring epidermal growth factor receptor (EGFR), phosphoinositide 3-kinase (PI3K), and the downstream formation of new integrin adhesions. This mechanism operates in addition to local cytoskeletal remodeling triggered by conformational changes in the E-cadherin-associated protein α-catenin, at sites of mechanical perturbation. Studies using magnetic twisting cytometry (MTC), together with traction force microscopy (TFM) and confocal imaging identified force-activated E-cadherin-specific signals that integrate cadherin force transduction, integrin activation and cell contractility. EGFR is required for the downstream activation of PI3K and myosin-II-dependent cell stiffening. Our findings also demonstrated that α-catenin-dependent cytoskeletal remodeling at perturbed E-cadherin adhesions does not require cell stiffening. These results broaden the repertoire of E-cadherin-based force transduction mechanisms, and define the force-sensitive signaling network underlying the mechano-chemical integration of spatially segregated adhesion receptors.

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Cited by

Sorted by citations

Authors
Muhamed, I., Wu, J., Sehgal, P., Kong, X., Tajik, A., Wang, N., & Leckband, D. E.
Affiliations
University of Illinois Urbana-Champaign (US)
ORCID
Venue
Journal of Cell Science
Publisher
The Company of Biologists
Published
2016-05-01
Volume
129
Issue
9
Pages
1843-1854
ISSN
  • 0021-9533 (ISSN-L)
  • 1477-9137
DOI
10.1242/jcs.185447
PMC
PMC4893802
OpenAlex
W2296441026
Cited by
97
References
3
Citations by year
2026: 6 · 2025: 13 · 2024: 5 · 2023: 9 · 2022: 9 · 2021: 9 · 2020: 8 · 2019: 7 · 2018: 12 · 2017: 15 · 2016: 4
FWCI
8.72
Type
journal article
Language
en
Open access
Licence
www.biologists.com/user-licence-1-1 (vor, 184 d)
Locations
  • Journal of Cell Science (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
  • HighWire Press Open Archive (submittedVersion)
Funders
  • National Institutes of Health (R01 GM097443)
  • National Science Foundation (1029871, 1462739)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular Mechanics and Interactions
  • Health Sciences › Medicine › Immunology and Allergy › Cell Adhesion Molecules Research
  • Physical Sciences › Physics and Astronomy › Atomic and Molecular Physics, and Optics › Force Microscopy Techniques and Applications
Keywords
E-cadherin, force transduction, cell mechanics, epidermal growth factor receptor, phosphatidylinositol 3-kinase, integrin-mediated adhesion, alpha-catenin, cytoskeletal remodeling, myosin II, cell contractility, mechanotransduction, magnetic twisting cytometry, traction force microscopy
MeSH terms
Animals, Cytoskeleton, Dogs, Humans, Cadherins, Mechanotransduction, Cellular, alpha Catenin, Madin Darby Canine Kidney Cells, MCF-7 Cells, ErbB Receptors

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