A Mechanism Underlying Preventive Effect of High-Intensity Training on Colon Cancer
Abstract
INTRODUCTION: We examined effects of high-intensity training on chemically induced aberrant crypt foci (ACF) in rat colon. We also investigated mechanisms that may underlie the results obtained, with a focus on secreted protein acidic and rich in cysteine (SPARC), which has been proposed as an exercise-related factor of colon cancer prevention. METHODS: After an administration of 1,2-dimethylhydrazine, F344 rats executed high-intensity intermittent swimming training (HIIST) (twelve 20-s swimming with a weight [16% body weight] with 10-s pauses between the bouts) 5 d·wk for 4 wk. The acute and chronic effects of the HIIST on SPARC were evaluated in rats. We evaluated the in vitro and in vivo effects of 5' AMP-activated protein kinase (AMPK) activator on SPARC in rat serum and epitrochlearis muscle. In human subjects, we determined serum SPARC after exhaustive bicycling consisting of six to seven bouts of exercise at 170% V˙O2max with 10-s rests between the bouts (high-intensity intermittent bicycling [HIIB]). The SPARC mRNA in human vastus lateralis was measured before and after the HIIB for 4 d·wk for 6 wk (HIIB-training [HIIBT]). RESULTS: The numbers of ACF were lower in the HIIST (47 ± 22) compared with the control (122 ± 47) rats (P < 0.05). SPARC in epitrochlearis and serum after HIIS of the trained rat was higher than that in the control resting rats. In vitro and vivo AMPK stimulation increased mRNA and SPARC protein in rat epitrochlearis, respectively. The human serum SPARC after the HIIB was elevated. SPARC mRNA in human muscle was elevated after the HIIBT. CONCLUSIONS: The results demonstrated that HIIST inhibits 1,2-dimethylhydrazine-induced colon ACF development. This effect may be explained by SPARC induction by the exercise intensity-related factor AMPK, potentially explaining the preventive effects of high-intensity intermittent exercise training against colon cancer.
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Exercise is associated with increased SPARC secretion in humans and animal models.
“Of note, exercise is associated with enhanced secreted protein acidic and rich in cysteine (SPARC) secretion in humans and animal models131;”
From Exerkines in health, resilience and disease (2022) · cites this paper as [131]
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Influential
- Authors
- MATSUO, K., SATO, K., SUEMOTO, K., MIYAMOTO-MIKAMI, E., FUKU, N., HIGASHIDA, K., TSUJI, K., XU, Y., LIU, X., IEMITSU, M., HAMAOKA, T., & TABATA, I.
- Affiliations
- Ritsumeikan University (JP)
- Juntendo University (JP)
- University of Shiga Prefecture (JP)
- ORCID
- Venue
- Medicine & Science in Sports & Exercise
- Published
- 2017-09
- Volume
- 49
- Issue
- 9
- Pages
- 1805-1816
- ISSN
- 0195-9131 (ISSN-L)
- OpenAlex
- W2610474086
- Cited by
- 39
- References
- 42
- Citations by year
- 2026: 1 · 2025: 2 · 2024: 5 · 2023: 5 · 2022: 8 · 2021: 6 · 2020: 3 · 2019: 3 · 2018: 5 · 2017: 1
- FWCI
- 3.13
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Medicine & Science in Sports & Exercise (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Japan Society for the Promotion of Science (15H03091, 17H02147, 16K13059, 17H02182, 13J08000)
- Topics
- Health Sciences › Medicine › Rehabilitation › Exercise and Physiological Responses
- Health Sciences › Medicine › Complementary and alternative medicine › Cardiovascular and exercise physiology
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Metabolism, Diabetes, and Cancer
- Keywords
- high-intensity intermittent training, colon cancer prevention, aberrant crypt foci, SPARC, AMPK, 1,2-dimethylhydrazine, physical activity, swimming training, cycling, muscle gene expression, serum biomarkers
- MeSH terms
- Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha, High-Intensity Interval Training, Animals, Colon, Colonic Neoplasms, Humans, Male, Oxidation-Reduction, Physical Conditioning, Animal, Rats, Inbred F344, Swimming, Blotting, Western, Osteonectin, Muscle, Skeletal, Models, Animal, Mitochondrial Proteins, Glucose Transporter Type 4, AMP-Activated Protein Kinases, Young Adult
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