The DOI matches its Crossref record; 7 of the 7 references it cites were found in a registry or an index.
Prp8 protein: At the heart of the spliceosome
Open accessAbstract
Pre-messenger RNA (pre-mRNA) splicing is a central step in gene expression. Lying between transcription and protein synthesis, pre-mRNA splicing removes sequences (introns) that would otherwise disrupt the coding potential of intron-containing transcripts. This process takes place in the nucleus, catalyzed by a large RNA–protein complex called the spliceosome. Prp8p, one of the largest and most highly conserved of nuclear proteins, occupies a central position in the catalytic core of the spliceosome, and has been implicated in several crucial molecular rearrangements that occur there. Recently, Prp8p has also come under the spotlight for its role in the inherited human disease, Retinitis Pigmentosa. Prp8 is unique, having no obvious homology to other proteins; however, using bioinformatical analysis we reveal the presence of a conserved RNA recognition motif (RRM), an MPN/JAB domain and a putative nuclear localization signal (NLS). Here, we review biochemical and genetical data, mostly related to the human and yeast proteins, that describe Prp8’s central role within the spliceosome and its molecular interactions during spliceosome formation, as splicing proceeds, and in post-splicing complexes.
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Cites
A comprehensive analysis of protein–protein interactions in Saccharomyces cerevisiae
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“Using the algorithm of Nakai (Nakai and Horton 1999), putative bipartite nuclear localization signals (NLS) are identifiable within 500 amino acids of the N terminus of Prp8 in all organisms.”
BackgroundMethodInfluentialStructural Basis of Transcription: RNA Polymerase II at 2.8 Ångstrom Resolution
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The highly reduced genome of an enslaved algal nucleus
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Cited by
The Spliceosome: Design Principles of a Dynamic RNP Machine
Wahl, M. C., Will, C. L., & Lührmann, R. (2009) · Cell · doi:10.1016/j.cell.2009.02.009 · Cited by 2,665
Spliceosome Structure and Function
Will, C. L., & Luhrmann, R. (2011) · Cold Spring Harbor Perspectives in Biology · doi:10.1101/cshperspect.a003707 · Cited by 1,829
Robust transcriptome-wide discovery of RNA-binding protein binding sites with enhanced CLIP (eCLIP)
Van Nostrand, E. L., Pratt, G. A., Shishkin, A. A., Gelboin-Burkhart, C., Fang, M. Y., Sundararaman, B., Blue, S. M., Nguyen, T. B., Surka, C., Elkins, K., et al. (2016) · Nature Methods · doi:10.1038/nmeth.3810 · Cited by 1,397
Furukawa, T., Kuboki, Y., Tanji, E., Yoshida, S., Hatori, T., Yamamoto, M., Shibata, N., Shimizu, K., Kamatani, N., & Shiratori, K. (2011) · Scientific Reports · doi:10.1038/srep00161 · Cited by 440
Structure of a yeast spliceosome at 3.6-angstrom resolution
Yan, C., Hang, J., Wan, R., Huang, M., Wong, C. C. L., & Shi, Y. (2015) · Science · doi:10.1126/science.aac7629 · Cited by 334
Metadata
- Authors
- GRAINGER, R. J., & BEGGS, J. D.
- Venue
- RNA
- Publisher
- Cold Spring Harbor Laboratory
- Published
- 2005-04-19
- Volume
- 11
- Issue
- 5
- Pages
- 533-557
- ISSN
- 1355-8382 (ISSN-L)
- 1469-9001
- OpenAlex
- W1979765627
- Cited by
- 372
- References
- 7
- FWCI
- 7.38
- Type
- journal article
- Language
- en
- Open access
- bronze
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