Identification of piRNAs in the central nervous system
Open accessAbstract
Piwi-interacting RNAs (piRNAs) are small noncoding RNAs generated by a conserved pathway. Their most widely studied function involves restricting transposable elements, particularly in the germline, where piRNAs are highly abundant. Increasingly, another set of piRNAs derived from intergenic regions appears to have a role in the regulation of mRNA from early embryos and gonads. We report a more widespread expression of a limited set of piRNAs and particularly focus on their expression in the hippocampus. Deep sequencing of extracted RNA from the mouse hippocampus revealed a set of small RNAs in the size range of piRNAs. These were confirmed by their presence in the piRNA database as well as coimmunoprecipitation with MIWI. Their expression was validated by Northern blot and in situ hybridization in cultured hippocampal neurons, where signal from one piRNA extended to the dendritic compartment. Antisense suppression of this piRNA suggested a role in spine morphogenesis. Possible targets include genes, which control spine shape by a distinctive mechanism in comparison to microRNAs.
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piRNAs are known to exist in the Drosophila and mammalian brain.
“While the function of piwi in regard to piRNA production and transposable element silencing is well established in the germline29, and piRNAs are known to exist in the Drosophila and mammalian brain30,31, it is currently unknown if piwi reduction affects transposable element transcripts in the brain.”
From Pathogenic tau-induced piRNA depletion promotes neuronal death through transposable element dysregulation in neurodegenerative tauopathies (2018) · cites this paper as [31]
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The Piwi-piRNA Pathway Provides an Adaptive Defense in the Transposon Arms Race
Aravin, A. A., Hannon, G. J., & Brennecke, J. (2007) · Science · doi:10.1126/science.1146484 · Cited by 1,096 · see citing works
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β-catenin mediates stress resilience through Dicer1/microRNA regulation
Dias, C., Feng, J., Sun, H., Shao, N. Y., Mazei-Robison, M. S., Damez-Werno, D., Scobie, K., Bagot, R., LaBonté, B., Ribeiro, E., et al. (2014) · Nature · doi:10.1038/nature13976 · Cited by 240 · see citing works
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Transcriptome-wide piRNA profiling in human brains of Alzheimer's disease
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Breast Cancer and the Other Non-Coding RNAs
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- Authors
- Lee, E. J., Banerjee, S., Zhou, H., Jammalamadaka, A., Arcila, M., Manjunath, B. S., & Kosik, K. S.
- Affiliations
- University of California, Santa Barbara (US)
- Venue
- RNA
- Publisher
- Cold Spring Harbor Laboratory
- Published
- 2011-04-22
- Volume
- 17
- Issue
- 6
- Pages
- 1090-1099
- ISSN
- 1355-8382 (ISSN-L)
- 1469-9001
- PMC
- PMC3096041
- OpenAlex
- W2150860105
- Cited by
- 289
- References
- 1
- Citations by year
- 2026: 5 · 2025: 8 · 2024: 6 · 2023: 13 · 2022: 21 · 2021: 32 · 2020: 25 · 2019: 19 · 2018: 27 · 2017: 25 · 2016: 19 · 2015: 19 · 2014: 24 · 2013: 19 · 2012: 21
- FWCI
- 26.61
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- RNA (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- HighWire Press Open Archive (submittedVersion)
- Funders
- National Science Foundation (0808772)
- California Institute for Regenerative Medicine
- Larry L. Hillblom Foundation
- Errett Fisher Foundation
- Topics
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Chromosomal and Genetic Variations
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Interference and Gene Delivery
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › Cancer-related molecular mechanisms research
- Keywords
- piRNA, hippocampus, central nervous system, small RNA, MIWI, spine morphogenesis, antisense inhibition, transposon silencing, mouse brain
- MeSH terms
- Animals, Central Nervous System, Female, Hippocampus, Male, Mice, Inbred C57BL, Spine, Blotting, Northern, In Situ Hybridization, RNA, Small Interfering, MicroRNAs, Mice, High-Throughput Nucleotide Sequencing
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