Cited by 46RNA and protein synthesis mechanismsBacterial Genetics and BiotechnologyRNA modifications and cancer

A novel single amino acid change in small subunit ribosomal protein S5 has profound effects on translational fidelity

Open access
Kirthi, Narayanaswamy, Roy-Chaudhuri, Biswajoy, Kelley, Teresa, Culver, Gloria M.·2006·RNA

Abstract

S5 is a small subunit ribosomal protein (r-protein) linked to the functional center of the 30S ribosomal subunit. In this study we have identified a unique amino acid mutation in Escherichia coli S5 that produces spectinomycin-resistance and cold sensitivity. This mutation significantly alters cell growth, folding of 16S ribosomal RNA, and translational fidelity. While translation initiation is not affected, both +1 and −1 frameshifting and nonsense suppression are greatly enhanced in the mutant strain. Interestingly, this S5 ribosome ambiguity-like mutation is spatially remote from previously identified S5 ribosome ambiguity (ram) mutations. This suggests that the mechanism responsible for ram phenotypes in the novel mutant strain is possibly distinct from those proposed for other known S5 (and S4) ram mutants. This study highlights the importance of S5 in ribosome function and cell physiology, and suggests that translational fidelity can be regulated in multiple ways.

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Integrity signals

Registry

Crossref · 10.1261/rna.302006

Cites

62 of 62 references verified0 citation errors · 0 not checkable

Statistics

Claims the paper makes

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Self-check

Percentages, sums and reported differences re-computed from the paper’s own numbers.

The self-check needs the paper’s full text.

Cites

Flagged first, then by citations

1 of the 63 references couldn’t be matched to a known paper.

Research works printed but not linked: 1

  1. Merryman, C. and Noller, H.F. 1998. Footprinting and modification- interference analysis of binding sites on RNA. In RNA:Protein interactions, A practical approach (ed. C.W.J. Smith), pp. 237-253. Oxford University Press, New York.

Cited by

Sorted by citations

Authors
Kirthi, N., Roy-Chaudhuri, B., Kelley, T., & Culver, G. M.
Affiliations
Iowa State University (US)
Venue
RNA
Published
2006-10-19
Volume
12
Issue
12
Pages
2080-2091
ISSN
  • 1355-8382 (ISSN-L)
  • 1469-9001
OpenAlex
W2159706908
Cited by
46
References
63
Citations by year
2026: 2 · 2025: 2 · 2023: 2 · 2022: 2 · 2021: 5 · 2018: 1 · 2017: 1 · 2016: 4 · 2015: 1 · 2014: 5 · 2012: 5
FWCI
1.50
Type
journal article
Language
en
Open access
Locations
  • RNA (publishedVersion)
  • Europe PMC (PubMed Central) (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • National Institutes of Health
  • National Institute of General Medical Sciences (R01GM062432, GM62432)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA and protein synthesis mechanisms
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA modifications and cancer
Keywords
ribosomal protein S5, amino acid mutations, translation fidelity, spectinomycin resistance, cold sensitivity, nonsense suppression, Escherichia coli
MeSH terms
Spectinomycin, Amino Acid Sequence, Escherichia coli, Glycine, Molecular Sequence Data, Mutation, Ribosomal Proteins, Ribosomes, RNA, Ribosomal, 16S, RNA, Transfer, Selection, Genetic, Protein Biosynthesis, Amino Acid Substitution, Drug Resistance, Bacterial, Escherichia coli Proteins

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review