Interaction of antibiotics with functional sites in 16S ribosomal RNA
Abstract
Chemical footprinting shows that several classes of antibiotics (streptomycin, tetracycline, spectinomycin, edeine, hygromycin and the neomycins) protect concise sets of highly conserved nucleotides in 16S ribosomal RNA when bound to ribosomes. These findings have strong implications for the mechanism of action of these antibiotics and for the assignment of functions to specific structural features of 16S rRNA.
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Hygromycin B binds close to the A site on the small subunit.
“and has been shown to bind close to the A site on the small subunit (Moazed and Noller 1987”
From The Structural Basis for the Action of the Antibiotics Tetracycline, Pactamycin, and Hygromycin B on the 30S Ribosomal Subunit (2000) · cites this paper as Moazed and Noller 1987
The shift of C1054 explains the increased reactivity of this base toward DMS.
“and, in the case of C1054, the shift explains the increased reactivity of this base toward DMS (Moazed and Noller 1987”
From The Structural Basis for the Action of the Antibiotics Tetracycline, Pactamycin, and Hygromycin B on the 30S Ribosomal Subunit (2000) · cites this paper as Moazed and Noller 1987
Biochemical studies have implicated both ribosomal proteins and 16S RNA in tetracycline binding.
“Biochemical studies have implicated both ribosomal proteins and 16S RNA in tetracycline binding (Moazed and Noller 1987; Buck and Cooperman 1990; Oehler et al. 1997”
From The Structural Basis for the Action of the Antibiotics Tetracycline, Pactamycin, and Hygromycin B on the 30S Ribosomal Subunit (2000) · cites this paper as Moazed and Noller 1987
Tetracycline directly interacts with A892.
“The direct interaction of Tc with A892 rationalizes the observed strong protection against attack by DMS at this position (Moazed and Noller 1987”
From The Structural Basis for the Action of the Antibiotics Tetracycline, Pactamycin, and Hygromycin B on the 30S Ribosomal Subunit (2000) · cites this paper as Moazed and Noller 1987
Binding of hygromycin B protects the N7 of 1494 from attack by DMS.
“binding of HygB protects the N7 of 1494 from attack by DMS (Moazed and Noller 1987”
From The Structural Basis for the Action of the Antibiotics Tetracycline, Pactamycin, and Hygromycin B on the 30S Ribosomal Subunit (2000) · cites this paper as Moazed and Noller 1987
Cites
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Crystal structure of the ribosome at 5.5 Å resolution
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“…factors and are thus able to a single binding site on the ribosome (Zierhut et al., 1979) and has been shown to bind close to the A site release the bound antibiotic from the ribosome (Mana-vathu et al., 1990; Burdett, 1996; Taylor and Chau, 1996). on the small subunit (Moazed and Noller, 1987).”
BackgroundCrystal structures of complexes of the small ribosomal subunit with tetracycline, edeine and IF3
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- Authors
- Moazed, D., & Noller, H. F.
- Affiliations
- University of California, Santa Cruz (US)
- Venue
- Nature
- Published
- 1987-06
- Volume
- 327
- Issue
- 6121
- Pages
- 389-394
- ISSN
- 0028-0836 (ISSN-L)
- 1476-4687
- OpenAlex
- W2018429609
- Cited by
- 1,150
- References
- 1
- Citations by year
- 2026: 10 · 2025: 7 · 2024: 23 · 2023: 20 · 2022: 19 · 2021: 17 · 2020: 27 · 2019: 28 · 2018: 30 · 2017: 20 · 2016: 21 · 2015: 25 · 2014: 34 · 2013: 32 · 2012: 26
- FWCI
- 10.05
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Nature (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA and protein synthesis mechanisms
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA modifications and cancer
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
- Keywords
- antibiotics, 16S rRNA, chemical footprinting, streptomycin, tetracycline, spectinomycin, edeine, hygromycin, neomycin, antibiotic binding sites, ribosome, antibiotic mechanism of action, conserved nucleotides
- MeSH terms
- Spectinomycin, Aminoglycosides, Anti-Bacterial Agents, Base Sequence, Cinnamates, Edeine, Hygromycin B, Nucleic Acid Conformation, RNA, Ribosomal, Streptomycin, Tetracycline
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