The DOI matches its Crossref record; 44 of the 44 references it cites were found in a registry or an index.
A quantitative analysis of intron effects on mammalian gene expression
Open accessAbstract
In higher eukaryotes, intron-containing and intronless versions of otherwise identical genes can exhibit dramatically different expression profiles. Introns and the act of their removal by the spliceosome can affect gene expression at many different levels, including transcription, polyadenylation, mRNA export, translational efficiency, and the rate of mRNA decay. However, the extent to which each of these steps contributes to the overall effect of any one intron on gene expression has not been rigorously tested. Here we report construction and initial characterization of a luciferase-based reporter system for monitoring the effects of individual introns and their position within the gene on protein expression in mammalian cells. Quantitative analysis of constructs containing human TPI intron 6 at two different positions within the Renilla luciferase open reading frame revealed that this intron acts primarily to enhance mRNA accumulation. Spliced mRNAs also exhibited higher translational yields than did intronless transcripts. However, nucleocytoplasmic mRNA distribution and mRNA stability were largely unaffected. These findings were extended to two other introns in a TCR-β minigene.
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Le Hir, H. (2001) · The EMBO Journal · doi:10.1093/emboj/20.17.4987 · Cited by 844
Introns increase gene expression in cultured maize cells.
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P-TEFb, a Cyclin-Dependent Kinase Controlling Elongation by RNA Polymerase II
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The protein Aly links pre-messenger-RNA splicing to nuclear export in metazoans
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Cited by
Nonsense-mediated mRNA decay: splicing, translation and mRNP dynamics
Maquat, L. E. (2004) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm1310 · Cited by 1,214
The power of the 3′ UTR: translational control and development.
Kuersten, S., & Goodwin, E. B. (2003) · Nature Reviews Genetics · doi:10.1038/nrg1125 · Cited by 530
Nonsense-mediated mRNA decay: terminating erroneous gene expression
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Are splicing mutations the most frequent cause of hereditary disease?
López-Bigas, N., Audit, B., Ouzounis, C., Parra, G., & Guigó, R. (2005) · FEBS Letters · doi:10.1016/j.febslet.2005.02.047 · Cited by 412
Introns: The Functional Benefits of Introns in Genomes
Jo, B.-S., & Choi, S. S. (2015) · Genomics & Informatics · doi:10.5808/gi.2015.13.4.112 · Cited by 359
Metadata
- Authors
- NOTT, A., MEISLIN, S. H., & MOORE, M. J.
- Venue
- RNA
- Publisher
- Cold Spring Harbor Laboratory
- Published
- 2003-05
- Volume
- 9
- Issue
- 5
- Pages
- 607-617
- ISSN
- 1355-8382 (ISSN-L)
- 1469-9001
- OpenAlex
- W2115744888
- Cited by
- 417
- References
- 44
- FWCI
- 6.86
- Type
- journal article
- Language
- en
- Open access
- bronze
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