Role of Xeroderma Pigmentosum Group D in Cell Cycle and Apoptosis in Cutaneous Squamous Cell Carcinoma A431 Cells
Open accessAbstract
BACKGROUND Cutaneous squamous cell carcinoma (cSCC) is the second most widespread cancer in humans and its incidence is rising. Novel therapy with better efficacy is needed for clinical treatment of cSCC. Many studies have shown the importance of DNA repair pathways during the development of cancer. A key nucleotide excision repair (NER) protein, xeroderma pigmentosum group D (XPD), is responsible for the excision of a large variety of bulky DNA lesions. MATERIAL AND METHODS To explore the role of XPD in A431 cells, we overexpressed XPD in A431 cells and performed MTT assay, flow cytometry, and Western blot analysis to examine cell proliferation, cell apoptosis, and genes expression. RESULTS We found that the overexpression of XPD suppressed cell viability, induced cell cycle arrest at G1 phase, and promoted cell apoptosis. Additionally, XPD blocked the expression of c-myc, cdc25A, and cdk2, and improved the levels of HIPK2 and p53. CONCLUSIONS These results provide new evidence to reveal the role of XPD in cSCC A431 cells and suggest that XPD may serve as an anti-oncogene during cSCC development.
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XPA and XPD are thought to have a dualistic nature and were shown to affect cell cycle checkpoint signaling.
“These include proteins of the NER system, such as XPA and XPD, that are thought to have a dualistic nature and were shown to affect cell cycle checkpoint signaling [73,74].”
From Focus on UV-Induced DNA Damage and Repair—Disease Relevance and Protective Strategies (2020) · cites this paper as [74]
Overexpression of XPD induces cell cycle arrest in G1 phase.
“In contrast, the overexpression of XPD was shown to induce cell cycle arrest in G1 phase [74].”
From Focus on UV-Induced DNA Damage and Repair—Disease Relevance and Protective Strategies (2020) · cites this paper as [74]
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Focus on UV-Induced DNA Damage and Repair—Disease Relevance and Protective Strategies
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“In contrast, the overexpression of XPD was shown to induce cell cycle arrest in G1 phase [74].”
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- Authors
- Liu, O.-G., Xiong, X.-Y., Li, C.-M., Zhou, X.-S., & Li, S.-S.
- Affiliations
- Second Affiliated Hospital of Nanchang University (CN)
- Department of Ophthalmology, The Second Affiliated Hospital of Nanchang University, Nanchang, Jiangxi, China (mainland)
- Department of Dermatology, The Second Affiliated Hospital of Nanchang University, Nanchang, Jiangxi, China (mainland)
- Corresponding author
- Liu, Ou-Gen
- Venue
- Medical Science Monitor
- Published
- 2018-01-24
- Volume
- 24
- Pages
- 453-460
- ISSN
- 1234-1010 (ISSN-L)
- 1643-3750
- PMC
- PMC5791386
- OpenAlex
- W2790473925
- Cited by
- 4
- References
- 35
- Citations by year
- 2026: 1 · 2024: 1 · 2021: 1 · 2020: 1
- FWCI
- 0.18
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- cc-by-nc-nd
- Locations
- Medical Science Monitor (publishedVersion, cc-by-nc-nd)
- PubMed Central (publishedVersion, cc-by-nc-nd)
- PubMed (publishedVersion)
- Funders
- Natural Science Foundation of Jiangxi Province
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › DNA Repair Mechanisms
- Health Sciences › Medicine › Epidemiology › Nonmelanoma Skin Cancer Studies
- Health Sciences › Medicine › Oncology › Cancer-related Molecular Pathways
- Keywords
- xeroderma pigmentosum group D, cutaneous squamous cell carcinoma, A431 cells, nucleotide excision repair, DNA repair, apoptosis, cell cycle arrest, G1 phase, cell proliferation, c-Myc, CDC25A, CDK2, HIPK2, p53, tumor suppressor
- MeSH terms
- Carcinoma, Squamous Cell, Cell Cycle, Humans, Skin Neoplasms, Xeroderma Pigmentosum, Gene Expression Regulation, Neoplastic, G1 Phase, Apoptosis, Cell Line, Tumor, Cell Proliferation, Cell Cycle Checkpoints
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