IMMUNOFLUORESCENT STUDIES ON THE LOCALIZATION OF TYROSINE HYDROXYLASE AND DOPAMINE-β-HYDROXYLASE IN THE MES-, DI-, AND TELENCEPHALON OF THE RAT USING UNPERFUSED FRESH FROZEN SECTIONS
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Distributions of tyrosine hydroxylase (TH) and dopamine-β-hydroxylase (DBH) in the rat brain were examined using immunofluorescent staining on unperfused fresh frozen sections. TH-positive and DBH-negative dopaminergic neurons were observed in the anterior hypothalamic nucleus. In other hypothalamic nuclei such as ventromedialis, dorsomedialis, and posterior hypothalamic nuclei, a few dopaminergic neurons were also observed.Our immunofluorescent results with unperfused fresh frozen sections indicated that TH or DBH was mainly localized in the cell bodies of catecholaminergic neurons and that small amounts of the enzymes were distributed in the nerve terminals. Although it had been reported that the amine enzymes existed in nerve terminals as well as in the cell bodies by using paraformaldehyde perfused sections, the fluorescence observed in nerve terminals might be produced not only by the amine enzymes but also by amines themselves.
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DRN TH+ neurons lack dopamine-β-hydroxylase and do not express 5-HT.
“Furthermore, DRN TH+ neurons lack dopamine-β-hydroxylase, which is necessary to convert DA to norepinephrine (Nagatsu et al., 1979), and do not express 5-hydroxytryptamine (5-HT) (Stratford and Wirtshafter, 1990).”
From Dorsal raphe dopamine neurons represent the experience of social isolation (2016) · cites this paper as Nagatsu et al., 1979
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Cespuglio, R. (1986) · Behavioral and Brain Sciences · doi:10.1017/s0140525x00046240
Reciprocal interaction revisited
Kilduff, T. S., & Guilleminault, C. (1986) · Behavioral and Brain Sciences · doi:10.1017/s0140525x00046379
The elusive sleep cycle generator
Henn, V. S. (1986) · Behavioral and Brain Sciences · doi:10.1017/s0140525x0004632x
Vasotocin: Neurohumoral control of the reciprocal-interaction model?
Normanton, J. R. (1986) · Behavioral and Brain Sciences · doi:10.1017/s0140525x00046434
Reciprocal interaction in sleep cycle control: Description, yes; explanation, no
van Dongen, P. A. M. (1986) · Behavioral and Brain Sciences · doi:10.1017/s0140525x00046501
Doubt and certainty in the neurophysiology of state
Henriksen, S. J. (1986) · Behavioral and Brain Sciences · doi:10.1017/s0140525x00046331
Krnjević, K. (1986) · Behavioral and Brain Sciences · doi:10.1017/s0140525x00046380
Histochemical Mapping of Vertebrate Brains for Study of Evolution
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van den Buuse, M., Wilson, K.-E., Payet, J. M., & Hale, M. W. (2025) · Neuroscience · doi:10.1016/j.neuroscience.2025.09.032
Catecholaminergic Brain Stem Regulatory Systems
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- Authors
- NAGATSU, I., INAGAKI, S., KONDO, Y., KARASAWA, N., & NAGATSU, T.
- Affiliations
- Department of Anatomy, School of Medicine, Fujita-Gakuen University
- Tokyo Institute of Technology (JP)
- Venue
- ACTA HISTOCHEMICA ET CYTOCHEMICA
- Published
- 1979
- Volume
- 12
- Issue
- 1
- Pages
- 20-37
- ISSN
- 0044-5991 (ISSN-L)
- 1347-5800
- OpenAlex
- W2018476398
- Cited by
- 98
- References
- 26
- Citations by year
- 2025: 2 · 2021: 2 · 2019: 1 · 2017: 1 · 2016: 1 · 2012: 2
- FWCI
- 1.59
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- ACTA HISTOCHEMICA ET CYTOCHEMICA (publishedVersion)
- Topics
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neurotransmitter Receptor Influence on Behavior
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
- Health Sciences › Medicine › Neurology › Parkinson's Disease Mechanisms and Treatments
- Keywords
- tyrosine hydroxylase, dopamine beta-hydroxylase, dopaminergic neurons, catecholaminergic neurons, hypothalamus, midbrain, diencephalon, telencephalon, enzyme localization, presynaptic terminals, rat brain
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