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Cited by 136Neuroscience and Neuropharmacology ResearchNeurotransmitter Receptor Influence on BehaviorMotor Control and Adaptation

Topographical distribution of dorsal and median raphe neurons projecting to motor, Sensorimotor, and visual cortical Areas in the rat

Waterhouse, Barry D., Mihailoff, Gregory A., Baack, Judy C., Woodward, Donald J.·1986·Journal of Comparative Neurology
DOI 10.1002/cne.902490403
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Abstract

Abstract The present study was conducted to examine the spatial organization of dorsal (DR) and median (MR) raphe neurons that project to rostrocaudally aligned areas of the rat cerebral cortex. An additional goal was to determine if individual DR cells that send efferents to forelimb sensorimotor or visual regions of the neocortex also send axon collaterals to forelimb (crus II) or visual (paraflocculus) areas of the cerebellum. Long‐Evans hooded rats received unilateral pressure injections of horseradish peroxidase (HRP) in either motor n = 4) or sensorimotor (n = 5) or visual (n = 4) cortex to determine the intranuclear location of DR and MR neurons that project to specific neocortical regions. Coronal sections (40‐100 μm) through the pons and midbrain were examined by light microscopy after the tetramethyl benzidine reaction and neutral red counterstaining were carried out. The locations of retrogradely labeled cells were recorded relative to a threedimensional biological coordinate system maintained by a computer linked to the light microscope. For double labeling studies, unilateral injections of fast blue and nuclear yellow were made in paired motor (sensorimotor cortex and crus II of the lateral cerebellum) or visual (cortical area 17 and paraflocculus) areas of the CNS. Coronal tissue sections (35 μm) were collected on coverslips and examined on a Leitz fluorescence microscope (wavelength = 365 nm). DR neurons labeled from cerebrocortical injections of HRP were concentrated in the rostral two‐thirds of the nucleus. HRP‐filled neurons were distributed such that individual groups of neurons projecting to motor, sensorimotor, or visual cortex were aligned in a partially overlapping, rostral to caudal array. In the dorsoventral dimension, retrogradely labeled cells were clustered in three distinct groupings such that neurons projecting to the motor, sensorimotor, and visual areas were concentrated in dorsal, intermediate, and ventral portions of the DR nucleus, respectively. For all cases, the majority of HRP‐filled cells were positioned along the midline or displaced to the side of the nucleus that was ipsilateral to the cortical injection site. A small number of retrogradely labeled neurons were observed in the MR following injections in the motor cortex. Computer‐assisted reconstruction of th z neuroanatomical data facilitated the visualization of spatial relationships between groups of DR neocortical projection neurons. Twenty to 30% of the cells labeled in the DR after paired injections of fast blue and nuclear yellow in sensorimotor or visual areas of the neocortex and cerebellum were doubled labeled indicating that individual DR neurons can send axon collaterals to functionally analogous regions of the CNS. Overall, the results indicate that, within the DR, a topographic ordering exists with respect to rostrocaudally aligned terminal fields in the neocortex. In addition, a portion of the DR cerebrocortical projection neurons also send axon collaterals to specific regions of the cerebellar cortex. The intranuclear organization observed in this study suggests that neuronal activity in distinct regions of the DR nucleus may independently influence discrete populations of cerebrocortical cells. Furthermore, the possibility must now be considered that a substantial number of single DR neurons provide a common input via axon collerals to portions of the neocortical and cerebellar circuitry that receive afferent information related to the same sensory or motor function.

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  • Most subcortical-projecting neurons were located in the dorsal DRN, whereas most cortical-projecting neurons were located in the ventral DRN.

    “Consistent with previous reports (Ren et al., 2018; Waterhouse et al., 1986), we found that most subcortical-projecting neurons were located in the dorsal DRN, whereas most cortical-projecting neurons were located in the ventral DRN.”

    From Molecular and anatomical organization of the dorsal raphe nucleus (2019) · cites this paper as Waterhouse et al., 1986

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Authors
Waterhouse, B. D., Mihailoff, G. A., Baack, J. C., & Woodward, D. J.
Affiliations
University of Texas Health Science Center at Dallas (US)
ORCID
Waterhouse, Barry D.
Venue
Journal of Comparative Neurology
Publisher
Wiley
Published
1986-07-22
Volume
249
Issue
4
Pages
460-476
ISSN
  • 0021-9967 (ISSN-L)
  • 1096-9861
DOI
10.1002/cne.902490403
OpenAlex
W2170771167
Cited by
136
References
41
Citations by year
2026: 1 · 2024: 2 · 2023: 2 · 2022: 1 · 2021: 4 · 2020: 6 · 2019: 4 · 2018: 3 · 2017: 2 · 2016: 3 · 2015: 5 · 2014: 4 · 2013: 5 · 2012: 6
FWCI
0.82
Type
journal article
Language
en
Open access
closed
Licence
onlinelibrary.wiley.com/termsAndConditions#vor (vor, 6654 d)
Locations
  • The Journal of Comparative Neurology (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • National Institute on Drug Abuse (DA-02338)
  • National Institute of Neurological Disorders and Stroke (NS-12644, NS-18081)
Topics
  • Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
  • Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neurotransmitter Receptor Influence on Behavior
  • Life Sciences › Neuroscience › Cognitive Neuroscience › Motor Control and Adaptation
Keywords
dorsal raphe nucleus, median raphe nucleus, raphe neurons, topographic organization, motor cortex, sensorimotor cortex, visual cortex, cerebellar Crus II, paraflocculus, axon collaterals, retrograde tracing, horseradish peroxidase, Fast Blue, thalamic nuclei
MeSH terms
Animals, Brain Mapping, Cell Count, Cerebral Cortex, Efferent Pathways, Fluorescent Dyes, Motor Cortex, Neurons, Raphe Nuclei, Rats, Inbred Strains, Somatosensory Cortex, Stereotaxic Techniques, Visual Cortex, Rats

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