Purification and Some Properties of Exo-type Fucoidanases from Vibrio sp. N-5
Abstract
Three fucoidanases were purified from Vibrio sp. N-5 by ammonium sulfate fractionation and chromatography with DEAE-Toyopearl 650 M, Sephacryl S-300 HR, and chromatofocusing. The purified enzymes gave a single band on disc polyacrylamide gel electrophoresis. The molecular weights of the enzymes, E-1, E-2, and E-3 were 39,500, 68,000, and 68,000, respectively, by SDS polyacrylamide gel electrophoresis and 158,000, 68,500, and 67,500 by gel filtration. The enzymes hydrolyzed gagome-fucoidan to give small oligosaccharides containing sulfate as main product.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
Fucoidanase activities were first detected in several different marine microorganisms, including marine bacteria such as Vibrio sp., Alteromonas sp., Pseudoalteromonas sp., and bacteria of the family Flavobacteriaceae.
“Fucoidanase activities were first detected in several different marine microorganisms, including marine bacteria such as Vibrio sp., Alteromonas sp., Pseudoalteromonas sp., and bacteria of the family Flavobacteriaceae.32,36,37,43”
From Fucoidans from brown seaweeds: an update on structures, extraction techniques and use of enzymes as tools for structural elucidation (2013) · cites this paper as [43]
Fucoidanase activities were first detected in several different marine microorganisms, including marine bacteria such as Vibrio sp., Alteromonas sp., Pseudoalteromonas sp., and bacteria of the family Flavobacteriaceae.
“Fucoidanase activities were first detected in several different marine microorganisms, including marine bacteria such as Vibrio sp., Alteromonas sp., Pseudoalteromonas sp., and bacteria of the family Flavobacteriaceae.32,36,37,43”
From Fucoidans from brown seaweeds: an update on structures, extraction techniques and use of enzymes as tools for structural elucidation (2013) · cites this paper as [37]
Cites
Still being checked.
Cited by
Sorted by citations
Berteau, O. (2003) · Glycobiology · doi:10.1093/glycob/cwg058 · Cited by 769 · see citing works
Chemical interactions between marine macroalgae and bacteria
Goecke, F., Labes, A., Wiese, J., & Imhoff, J. (2010) · Marine Ecology Progress Series · doi:10.3354/meps08607 · Cited by 474 · see citing works
Ale, M. T., & Meyer, A. S. (2013) · RSC Adv. · doi:10.1039/c3ra23373a · Cited by 381 · see citing works
Research and Application of Marine Microbial Enzymes: Status and Prospects
Zhang, C., & Kim, S. (2010) · Marine Drugs · doi:10.3390/md8061920 · Cited by 232 · see citing works
Structure, biological activity, and enzymatic transformation of fucoidans from the brown seaweeds
Kusaykin, M., Bakunina, I., Sova, V., Ermakova, S., Kuznetsova, T., Besednova, N., Zaporozhets, T., & Zvyagintseva, T. (2008) · Biotechnology Journal · doi:10.1002/biot.200700054 · Cited by 197 · see citing works
Holtkamp, A. D., Kelly, S., Ulber, R., & Lang, S. (2009) · Applied Microbiology and Biotechnology · doi:10.1007/s00253-008-1790-x · Cited by 175 · see citing works
Pomin, V. H., Pereira, M. S., Valente, A.-P., Tollefsen, D. M., Pavão, M. S. G., & Mourão, P. A. S. (2005) · Glycobiology · doi:10.1093/glycob/cwi021 · Cited by 129 · see citing works
Hydrolysis of Fucoidan by Fucoidanase Isolated from the Marine Bacterium, Formosa algae
Silchenko, A. S., Игоревич, К. М., Kurilenko, V. V., Zakharenko, A. M., Isakov, V. V., Zaporozhets, T. S., Константиновна, Г. А., & Николаевна, З. Т. (2013) · Marine Drugs · doi:10.3390/md11072413 · Cited by 96 · see citing works
Marine glycosyl hydrolases in the hydrolysis and synthesis of oligosaccharides
Giordano, A., Andreotti, G., Tramice, A., & Trincone, A. (2006) · Biotechnology Journal · doi:10.1002/biot.200500036 · Cited by 77 · see citing works
Kim, W. J., Koo, Y. K., Jung, M.-K., Moon, H. R., Kim, S. M., Synytsya, A., Yun‐Choi, H. S., Kim, Y. S., Park, J. K., & Park, Y. I. (2010) · Archives of Pharmacal Research · doi:10.1007/s12272-010-2234-6 · Cited by 76 · see citing works
Isolation and characterization of a sea cucumber fucoidan-utilizing marine bacterium
Chang, Y., Xue, C., Tang, Q., Li, D., Wu, X., & Wang, J. (2009) · Letters in Applied Microbiology · doi:10.1111/j.1472-765x.2009.02792.x · Cited by 72 · see citing works
Degradation of Fucoidan by the Marine Proteobacterium Pseudoalteromonas citrea
Bakunina, I. Y., Nedashkovskaya, O. I., Alekseeva, S. A., Ivanova, E. P., Romanenko, L. A., Gorshkova, N. M., Isakov, V. V., Zvyagintseva, T. N., & Mikhailov, V. V. (2002) · Microbiology · doi:10.1023/a:1017994131769 · Cited by 70 · see citing works
Silchenko, A. S., Rasin, A. B., Игоревич, К. М., Malyarenko, O. S., Shevchenko, N. M., Zueva, A. O., Kalinovsky, A. I., Николаевна, З. Т., & Ermakova, S. P. (2018) · Carbohydrate Polymers · doi:10.1016/j.carbpol.2018.03.094 · Cited by 68 · see citing works
Marine microbes as a valuable resource for brand new industrial biocatalysts
Beygmoradi, A., & Homaei, A. A. (2017) · Biocatalysis and Agricultural Biotechnology · doi:10.1016/j.bcab.2017.06.013 · Cited by 68 · see citing works
Two Species of Culturable Bacteria Associated With Degradation of Brown Algae Fucus Evanescens
Ivanova, E. P., Bakunina, I. Y., Sawabe, T., Hayashi, K., Alexeeva, Y. V., Zhukova, N. V., Nicolau, D. V., Zvaygintseva, T. N., & Mikhailov, V. V. (2002) · Microbial Ecology · doi:10.1007/s00248-001-1011-y · Cited by 65 · see citing works
Игоревич, К. М., Silchenko, A. S., Zakharenko, A. M., & Николаевна, З. Т. (2015) · Glycobiology · doi:10.1093/glycob/cwv072 · Cited by 60 · see citing works
Rodríguez‐Jasso, R. M., Mussatto, S. I., Sepúlveda, L., Torrado, A., Pastrana, L. M., Aguilar, C. N., & Teixeira, J. A. (2013) · Food and Bioproducts Processing · doi:10.1016/j.fbp.2013.02.004 · Cited by 56 · see citing works
Polysaccharide-degrading enzymes from marine bacteria
Michel, G., & Czjzek, M. (2013) · Elsevier eBooks · doi:10.1533/9781908818355.3.429 · Cited by 55 · see citing works
Li, B., Liu, J., Zhou, S., Fu, L., Yao, P., Chen, L., Yang, Z., Wang, X., & Zhang, X. (2019) · Marine Life Science & Technology · doi:10.1007/s42995-019-00003-4 · Cited by 43 · see citing works
Cao, H. T. T., Mikkelsen, M. D., Lezyk, M. J., Bui, L. M., Vân, T. T. T., Silchenko, A. S., Игоревич, К. М., Thinh, P. D., Truong, H. B., Holck, J., et al. (2018) · Marine Drugs · doi:10.3390/md16110422 · Cited by 42 · see citing works
Rodríguez‐Jasso, R. M., Mussatto, S. I., Pastrana, L. M., Aguilar, C. N., & Teixeira, J. A. (2010) · Applied Biochemistry and Biotechnology · doi:10.1007/s12010-010-8992-2 · Cited by 42 · see citing works
Application of Marine Microbial Enzymes in the Food and Pharmaceutical Industries
Zhang, C., & Kim, S. (2012) · Advances in food and nutrition research · doi:10.1016/b978-0-12-416003-3.00028-7 · Cited by 41 · see citing works
Purification and characteristics of fucoidanase obtained from Dendryphiella arenaria TM94
Wu, Q., Zhang, M., Wu, K., Liu, B., Cai, J., & Renrui, P. (2010) · Journal of Applied Phycology · doi:10.1007/s10811-010-9588-5 · Cited by 40 · see citing works
Chitin stimulates production of the antibiotic andrimid in a Vibrio coralliilyticus strain
Wietz, M., Månsson, M., & Gram, L. (2011) · Environmental Microbiology Reports · doi:10.1111/j.1758-2229.2011.00259.x · Cited by 39 · see citing works
Игоревич, К. М., Burtseva, Y. V., Svetasheva, T. G., Сова, В. В., & Николаевна, З. Т. (2003) · Biochemistry (Moscow) · doi:10.1023/a:1023058301392 · Cited by 36 · see citing works
Effective production of fermentable sugars from brown macroalgae biomass
Wang, D., Kim, D. H., & Kim, K. H. (2016) · Applied Microbiology and Biotechnology · doi:10.1007/s00253-016-7857-1 · Cited by 34 · see citing works
Screening of marine bacteria for fucoidanases
Bakunina, I. Y., Шевченко, Л. С., Nedashkovskaya, O. I., Шевченко, Н. М., Alekseeva, S. A., Mikhailov, V. V., & Николаевна, З. Т. (2000) · Microbiology · doi:10.1007/bf02756738 · Cited by 33 · see citing works
A recent update on fucoidonase: source, Isolation methods and its enzymatic activity
Barzkar, N., Rungsardthong, V., Jahromi, S. T., Laraib, Q., Das, R., Babich, O., & Сухих, С. А. (2023) · Frontiers in Marine Science · doi:10.3389/fmars.2023.1129982 · Cited by 32 · see citing works
Urvantseva, A. M., Bakunina, I. Y., Nedashkovskaya, O. I., Kim, S. B., & Николаевна, З. Т. (2006) · Applied Biochemistry and Microbiology · doi:10.1134/s0003683806050073 · Cited by 32 · see citing works
Bilan, M. I., Kusaykin, M. I., Grachev, A. A., Tsvetkova, E. A., Zvyagintseva, T. N., Nifantiev, N. E., & Usov, A. I. (2005) · Biochemistry (Moscow) · doi:10.1007/s10541-005-0264-3 · Cited by 30 · see citing works
Kim, W., Park, J., Park, J., Choi, D. J., & Park, Y. I. (2015) · Marine Drugs · doi:10.3390/md13074398 · Cited by 29 · see citing works
Proteomics meets blue biotechnology: A wealth of novelties and opportunities
Hartmann, E. M., Durighello, E., Pible, O., Nogales, B., Beltrametti, F., Bosch, R., Christie‐Oleza, J. A., & Armengaud, J. (2014) · Marine Genomics · doi:10.1016/j.margen.2014.04.003 · Cited by 28 · see citing works
Optimization of glycosidases production by Pseudoalteromonas issachenkonii KMM 3549T
Alexeeva, Y. V., Ivanova, E. P., Bakunina, I. Y., Zvaygintseva, T. N., & Mikhailov, V. V. (2002) · Letters in Applied Microbiology · doi:10.1046/j.1472-765x.2002.01189.x · Cited by 26 · see citing works
Purification and the Secondary Structure of Fucoidanase from Fusarium sp. LD8
Wu, Q., Ma, S., Hourong, X., Zhang, M., & Jing-min, C. (2011) · Evidence-based Complementary and Alternative Medicine · doi:10.1155/2011/196190 · Cited by 24 · see citing works
Purification and biochemical characterization of a novel α-glucosidase from Aspergillus niveus
da Silva, T. M., Michelin, M., Damásio, A., Maller, A., Almeida, F. B. D. R., Ruller, R., Ward, R. J., Rosa, J. C., Jorge, J. A., Terenzi, H. F., et al. (2009) · Antonie van Leeuwenhoek · doi:10.1007/s10482-009-9372-1 · Cited by 23 · see citing works
Fucoidanase inhibitory activity of phlorotannins from brown algae
Imbs, T. I., Silchenko, A. S., Федореев, С. А., Isakov, V. V., Ermakova, S. P., & Николаевна, З. Т. (2018) · Algal Research · doi:10.1016/j.algal.2018.03.009 · Cited by 18 · see citing works
Jagtap, A. S., Sankar, N. P. V., Ghori, R. I., & Manohar, C. S. (2022) · Folia Microbiologica · doi:10.1007/s12223-021-00943-4 · Cited by 18 · see citing works
Zhao, Y., Ning, L., Zhu, P., Jiang, J., Yao, Z., & Zhu, B. (2025) · Marine Drugs · doi:10.3390/md23030097 · Cited by 17 · see citing works
Liu, S., Wang, Q., Shao, Z., Liu, Q., He, Y., Ren, D., Yang, H., & Li, X. (2023) · Foods · doi:10.3390/foods12071555 · Cited by 16 · see citing works
Zayed, A., Cao, H. T. T., Trang, V. T. D., & Ulber, R. (2023) · Journal of Applied Phycology · doi:10.1007/s10811-023-03036-6 · Cited by 16 · see citing works
O’Connell, E., Murray, P., Piggott, C. O., Hennequart, F., & Tuohy, M. G. (2008) · Journal of Applied Phycology · doi:10.1007/s10811-007-9291-3 · Cited by 12 · see citing works
Distribution of Fucoidan Hydrolases and Some Glycosidases among Marine Invertebrates
Burtseva, Y. V., Кусайкин, М. И., Сова, В. В., Шевченко, Н. М., Skobun, A. S., & Николаевна, З. Т. (2000) · Russian Journal of Marine Biology · doi:10.1023/a:1009459107956 · Cited by 12 · see citing works
Bioprospects of Microbial Enzymes from Mangrove-Associated Fungi and Bacteria
Saravanakumar, K., Rajendran, N., Kathiresan, K., & Chen, J. (2016) · Advances in food and nutrition research · doi:10.1016/bs.afnr.2016.08.003 · Cited by 11 · see citing works
Wang, J., Liu, Z., Pan, X., Wang, N., Li, L., Du, Y., Li, J., & Li, M. (2022) · Marine Drugs · doi:10.3390/md20080533 · Cited by 10 · see citing works
Isolation and characterization of a fucoidan-degrading bacterium from Laminaria japonica
Wāng, Y., Li, B., Zhao, X., & Piao, M. (2013) · Journal of Ocean University of China · doi:10.1007/s11802-014-2011-0 · Cited by 9 · see citing works
A Simple Plate Method for the Screening and Detection of Fucoidanases
Silchenko, A. S., Khánh, H. H. N., Cao, H. T. T., Kurilenko, V. V., Zakharenko, A. M., Zueva, A. O., Lý, B. M., & Игоревич, К. М. (2015) · Achievements in the Life Sciences · doi:10.1016/j.als.2015.12.002 · Cited by 9 · see citing works
Li, Q., Jiang, C., Tan, H., Zhao, X., Li, K., & Yin, H. (2021) · Protein Expression and Purification · doi:10.1016/j.pep.2021.105897 · Cited by 6 · see citing works
Identification and evaluation of fungal strains with fucoidan degradation potential
Rodríguez‐Jasso, R. M., González, C., Pastrana, L. M., & Teixeira, J. A. (2008) · Portuguese National Funding Agency for Science, Research and Technology (RCAAP Project by FCT) · Cited by 4 · see citing works
Progress in the study of fucoidan degradation
Lv, H., Su, M., Hao, X., Li, K., Zhang, X., Wang, Y., Tian, X., Wang, Z., Qiao, Z., Li, X., et al. (2025) · Carbohydrate Research · doi:10.1016/j.carres.2025.109640 · Cited by 2 · see citing works
Garcia‐Ruiz, E., Badur, A. H., Rao, C. V., & Zhao, H. (2016) · Green chemistry and sustainable technology · doi:10.1007/978-3-662-53704-6_5 · Cited by 2 · see citing works
Omuzbüken, B., Alyürük, H., & Kaçar, A. (2024) · Research Journal of Chemistry and Environment · doi:10.25303/286rjce45051 · Cited by 1 · see citing works
Biocatalysts from Aplysia: Sweet Spot in Enzymatic Carbohydrate Synthesis
Trincone, A. (2015) · Springer eBooks · doi:10.1007/978-3-642-53971-8_30
O’Connell, E., Murray, P., Piggott, C. O., Hennequart, F., & Tuohy, M. G. (2009) · Springer eBooks · doi:10.1007/978-1-4020-9619-8_15
- Authors
- Furukawa, S.-I., Fujikawa, T., Koga, D., & Ide, A.
- Affiliations
- Hijiyama University Junior College (JP)
- Yamaguchi University (JP)
- ORCID
- Ide, Akio
- Venue
- Bioscience, Biotechnology, and Biochemistry
- Publisher
- Oxford University Press (OUP)
- Published
- 1992-01
- Volume
- 56
- Issue
- 11
- Pages
- 1829-1834
- ISSN
- 0916-8451 (ISSN-L)
- 1347-6947
- OpenAlex
- W2161843191
- Cited by
- 54
- References
- 0
- Citations by year
- 2025: 2 · 2024: 1 · 2023: 3 · 2022: 2 · 2021: 1 · 2019: 1 · 2018: 3 · 2017: 2 · 2016: 3 · 2015: 4 · 2014: 1 · 2013: 5 · 2012: 1
- FWCI
- 0.00
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Bioscience Biotechnology and Biochemistry (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biotechnology › Enzyme Production and Characterization
- Life Sciences › Agricultural and Biological Sciences › Aquatic Science › Seaweed-derived Bioactive Compounds
- Life Sciences › Agricultural and Biological Sciences › Food Science › Polysaccharides Composition and Applications
- Keywords
- fucoidanase, enzyme purification, molecular weight determination, polysaccharide degradation
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
