Effect of Enzyme Preparation from the Marine Mollusk Littorina kurila on Fucoidan from the Brown Alga Fucus distichus
Abstract
A fucoidanase preparation from the marine mollusk Littorina kurila cleaved some glycosidic bonds in fucoidan from the brown alga Fucus distichus, but neither fucose nor lower oligosaccharides were produced. The main product isolated from the incubation mixture was a polysaccharide built up of disaccharide repeating units -->3)-alpha-L-Fucp-(2,4-di-SO3(-))-(1-->4)-alpha-L-Fucp-(2SO3(-))-(1-->, the structure coinciding with the idealized formula proposed for the initial substance. A polymer fraction with the same carbohydrate chain but sulfated only at positions 2 and nonstoichiometrically acetylated at positions 3 and 4 of fucose residues was isolated as a minor component. It is suggested that the native polysaccharide should contain small amounts of non-sulfated and non-acetylated fucose residues, and only their glycosidic bonds are cleaved by the enzyme. The enzymatic hydrolysis showed that irregular regions of the native polysaccharide containing acetylated and partially sulfated repeating units were assembled in blocks.
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Native fucoidan polysaccharides from Fucus distichus contain blocks of acetylated and partially sulfated repeating units.
“A study of the fucosidase action on fucoidan polysaccharides from Fucus distichus has thus established that the native fucoidan polysaccharides contain blocks of acetylated and partially sulfated repeating units.35”
From Fucoidans from brown seaweeds: an update on structures, extraction techniques and use of enzymes as tools for structural elucidation (2013) · cites this paper as [35]
Fucoidanases have been intensely studied in relation to bacterial degradation of fucoidans and molecular structure elucidation of fucoidan polysaccharides.
“hand, fucoidanases have been intensely studied in relation to bacterial degradation of fucoidans and molecular structure elucidation of fucoidan polysaccharides.35,36,42”
From Fucoidans from brown seaweeds: an update on structures, extraction techniques and use of enzymes as tools for structural elucidation (2013) · cites this paper as [35]
The main fucoidan structural unit from Fucus spp. consists of repeating di-saccharide units of [A3)-a-L-Fucp-(2,4di-SO3^2)-(1A4)-a-L-Fucp-(2SO3^2)-(1A].
“Likewise, the enzymatic treatment confirmed that the main fucoidan structural unit from this brown seaweed Fucus spp.consists of repeating di-saccharide units of [A3)-a-L-Fucp-(2,4di-SO 3 2 )-(1A4)-a-L-Fucp-(2SO 3 2 )-(1A].35”
From Fucoidans from brown seaweeds: an update on structures, extraction techniques and use of enzymes as tools for structural elucidation (2013) · cites this paper as [35]
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- Authors
- Bilan, M. I., Kusaykin, M. I., Grachev, A. A., Tsvetkova, E. A., Zvyagintseva, T. N., Nifantiev, N. E., & Usov, A. I.
- Affiliations
- Russian Academy of Sciences (RU)
- Corresponding author
- Usov, A. I.
- Venue
- Biochemistry (Moscow)
- Published
- 2005-12
- Volume
- 70
- Issue
- 12
- Pages
- 1321-1326
- ISSN
- 0006-2979 (ISSN-L)
- 1608-3040
- OpenAlex
- W2115220583
- Cited by
- 30
- References
- 22
- Citations by year
- 2025: 3 · 2024: 1 · 2023: 1 · 2022: 1 · 2021: 2 · 2020: 3 · 2019: 1 · 2018: 2 · 2016: 3 · 2015: 2 · 2014: 3 · 2013: 3
- FWCI
- 1.00
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- Locations
- Biochemistry (Moscow) (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Aquatic Science › Seaweed-derived Bioactive Compounds
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Polysaccharides and Plant Cell Walls
- Life Sciences › Agricultural and Biological Sciences › Food Science › Polysaccharides Composition and Applications
- Keywords
- fucoidanase, Fucus distichus, fucoidan, enzymatic hydrolysis, polysaccharide structure, brown algae polysaccharides
- MeSH terms
- Animals, Carbohydrate Sequence, Glycoside Hydrolases, Mollusca, Polysaccharides, Nuclear Magnetic Resonance, Biomolecular, Fucus
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