Cited by 30Seaweed-derived Bioactive CompoundsPolysaccharides and Plant Cell WallsPolysaccharides Composition and Applications

Effect of Enzyme Preparation from the Marine Mollusk Littorina kurila on Fucoidan from the Brown Alga Fucus distichus

Bilan, M. I., Kusaykin, M. I., Grachev, A. A., Tsvetkova, E. A., Zvyagintseva, T. N., Nifantiev, N. E., Usov, A. I.·2005·Biochemistry (Moscow)

Abstract

A fucoidanase preparation from the marine mollusk Littorina kurila cleaved some glycosidic bonds in fucoidan from the brown alga Fucus distichus, but neither fucose nor lower oligosaccharides were produced. The main product isolated from the incubation mixture was a polysaccharide built up of disaccharide repeating units -->3)-alpha-L-Fucp-(2,4-di-SO3(-))-(1-->4)-alpha-L-Fucp-(2SO3(-))-(1-->, the structure coinciding with the idealized formula proposed for the initial substance. A polymer fraction with the same carbohydrate chain but sulfated only at positions 2 and nonstoichiometrically acetylated at positions 3 and 4 of fucose residues was isolated as a minor component. It is suggested that the native polysaccharide should contain small amounts of non-sulfated and non-acetylated fucose residues, and only their glycosidic bonds are cleaved by the enzyme. The enzymatic hydrolysis showed that irregular regions of the native polysaccharide containing acetylated and partially sulfated repeating units were assembled in blocks.

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Crossref · 10.1007/s10541-005-0264-3

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Authors
Bilan, M. I., Kusaykin, M. I., Grachev, A. A., Tsvetkova, E. A., Zvyagintseva, T. N., Nifantiev, N. E., & Usov, A. I.
Affiliations
Russian Academy of Sciences (RU)
Corresponding author
Usov, A. I.
Venue
Biochemistry (Moscow)
Published
2005-12
Volume
70
Issue
12
Pages
1321-1326
ISSN
  • 0006-2979 (ISSN-L)
  • 1608-3040
OpenAlex
W2115220583
Cited by
30
References
22
Citations by year
2025: 3 · 2024: 1 · 2023: 1 · 2022: 1 · 2021: 2 · 2020: 3 · 2019: 1 · 2018: 2 · 2016: 3 · 2015: 2 · 2014: 3 · 2013: 3
FWCI
1.00
Type
journal article
Language
en
Open access
closed
Locations
  • Biochemistry (Moscow) (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Agricultural and Biological Sciences › Aquatic Science › Seaweed-derived Bioactive Compounds
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Polysaccharides and Plant Cell Walls
  • Life Sciences › Agricultural and Biological Sciences › Food Science › Polysaccharides Composition and Applications
Keywords
fucoidanase, Fucus distichus, fucoidan, enzymatic hydrolysis, polysaccharide structure, brown algae polysaccharides
MeSH terms
Animals, Carbohydrate Sequence, Glycoside Hydrolases, Mollusca, Polysaccharides, Nuclear Magnetic Resonance, Biomolecular, Fucus

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